AB169175

Anti-Wnt3a antibody

Name: Anti-Wnt3a antibody (ab169175) | Abcam
Brand: Abcam Limited
SKU: AB169175
Price: 545 USD
Availability: InStock

Be the first to review this product! Submit a review

(2 Publications)

Mouse Polyclonal WNT3A antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human WNT3A.

View Alternative Names

Protein Wnt-3a, WNT3A

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody (AB169175)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody (AB169175)

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells labeling Wnt3a with ab169175 at 10μg/ml.

Unknown

Western blot - Anti-Wnt3a antibody (AB169175)

All lanes:

Western blot - Anti-Wnt3a antibody (ab169175) at 1 µg/mL

All lanes:

Human pancreas tissue lysate at 50 µg

Predicted band size: 39 kDa

false

Unknown

Western blot - Anti-Wnt3a antibody (AB169175)

All lanes:

Western blot - Anti-Wnt3a antibody (ab169175) at 1 µg/mL

Lane 1:

Wnt3a transfected lysate (42.35 KDa) at 15 µL

Lane 2:

Non-transfected lysate at 15 µL

Predicted band size: 39 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human WNT3A.

P56704

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Wnt3a protein also known as Wingless-related integration site 3a is a member of the Wnt family of proteins. It has a molecular mass of approximately 38 kDa. Wnt3a is a secreted glycoprotein that plays a mechanical role in cell signaling. It is expressed in various tissues including the neural tube somites and limb buds in the developing embryo. The protein functions by binding to Frizzled receptors on the cell surface initiating a signaling cascade that influences cellular behaviors.

Biological function summary

Wnt3a plays a critical role in embryonic development and cell proliferation. As part of a larger complex it partners with other signaling molecules to ensure proper cellular communication. Wnt3a is important in patterning during embryogenesis influencing processes like axis formation and organogenesis. Its expression impacts the development of skeletal structures by regulating the differentiation and proliferation of precursor cells.

Pathways

Wnt3a is an integral component of the canonical Wnt/β-catenin signaling pathway which regulates gene expression essential for cell fate decisions. This pathway is activated when Wnt3a binds to the Frizzled and LRP5/6 receptor complex stabilizing β-catenin in the cytosol. Glycogen synthase kinase 3 beta (GSK-3β) and Adenomatosis polyposis coli (APC) are key proteins involved which help mediate the signal to downstream targets involved in cell growth and development.

Wnt3a has significant associations with cancer and bone developmental disorders. Dysregulation of the Wnt3a signaling pathway can lead to tumorigenesis due to unchecked cellular proliferation and survival. In cancer components like β-catenin become targets for therapeutic interventions. In bone disorders improperly regulated Wnt3a expression can result in conditions like osteoporosis where the balance between bone formation and resorption is disrupted. FGFR2 a protein involved in skeletal abnormalities often interacts with dysregulated Wnt3a signaling highlighting its importance in bone health.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 20093360, PubMed : 21244856, PubMed : 24841207, PubMed : 26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed : 26902720).

See full target information WNT3A

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Brazilian journal of medical and biological resear 51:e6997 PubMed29694513

2018

miR-27a protects human mitral valve interstitial cell from TNF-α-induced inflammatory injury via up-regulation of NELL-1.

Applications

Unspecified application

Species

Unspecified reactive species

Honglei Chen,Zhixu Zhang,Li Zhang,Junzhi Wang,Minghui Zhang,Bin Zhu

BMC cell biology 19:1 PubMed29433423

2018

MicroRNA-17-5p aggravates lipopolysaccharide-induced injury in nasal epithelial cells by targeting Smad7.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Huang,Wenjing Li,Xiaolong Wang,Shanshan Qi

View all publications

style

left-column

style

left-column

style

right-column