Rabbit Polyclonal WNT3A antibody. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 19 publications.
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- Dentistry journal 11:2023Characterization of SHH, SOX3, WNT3A and WNT9B Proteins in Human Non-Syndromic Cleft Lip and Palate Tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMārtiņš Vaivads,Ilze Akota,Māra PilmanePubMed 37366674
- Experimental animals 72:132-1392022The Philippines stingless bee propolis promotes hair growth through activation of Wnt/β-catenin signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulan Tang,Chen Wang,Mark Joseph M Desamero,Mun Keong Kok,James Kenn Chambers,Kazuyuki Uchida,Yuri Kominami,Hideki Ushio,Cleofas Cervancia,Maria Amelita Estacio,Shigeru Kyuwa,Shigeru KakutaPubMed 36310084
- Journal of oral biology and craniofacial research 12:721-7262022Amelogenin as a regenerative endodontic molecule for immature teeth with apical periodontitis. An experimental study.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaha M F Mounir,Fatma M Rashed,Sahar M BukharyPubMed 36097610
- The European journal of neuroscience 55:5-172021Nasal obstruction during the growth period modulates the Wnt/β-catenin pathway and brain-derived neurotrophic factor production in association with tyrosine kinase receptor B mRNA reduction in mouse hippocampus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHideyuki Ishidori,Hidemasa Okihara,Takuya Ogawa,Yasunori Abe,Chiho Kato,Phyo Thura Aung,Akiyo Fujita,Satoshi Kokai,Takashi OnoPubMed 34842314
- Journal of applied toxicology : JAT 42:285-2942021The neuroprotective effects of alpha-lipoic acid on an experimental model of Alzheimer's disease in PC12 cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXinrong Pei,Fangyan Hu,Feiya Luo,Xianglu Huang,Xiaoling Li,Shuxia Xing,Dingxin LongPubMed 34133789
- Diseases (Basel, Switzerland) 9:2021Cleft Candidate Genes and Their Products in Human Unilateral Cleft Lip Tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMārtiņš Vaivads,Ilze Akota,Māra PilmanePubMed 33917041
- Molecular biology of the cell 31:1703-17132020Three-dimensional niche stiffness synergizes with Wnt7a to modulate the extent of satellite cell symmetric self-renewal divisions.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLouise A Moyle et. al.PubMed 32491970
- OncoTargets and therapy 12:9201-92132019CLK3 Is A Direct Target Of miR-144 And Contributes To Aggressive Progression In Hepatocellular Carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHua Li,Xichun Cui,Qiuyue Hu,Xiaolong Chen,Pengli ZhouPubMed 31807004
- Cancer cell international 19:2712019Inactivation of the Wnt/β-catenin signaling pathway underlies inhibitory role of microRNA-129-5p in epithelial-mesenchymal transition and angiogenesis of prostate cancer by targeting ZIC2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenming Jiang et. al.PubMed 31649488
- Nature communications 10:14052019Wnt1 silences chemokine genes in dendritic cells and induces adaptive immune resistance in lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDimitra Kerdidani et. al.PubMed 30926812
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Predicted | Predicted |
| Mouse | Tested | Tested |
| Chicken | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Human, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes Can be blocked with Wnt3a peptide (ab23326). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Human, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
Associated Products
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4 products for Alternative Product
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:20093360, PubMed:21244856, PubMed:24841207, PubMed:26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed:26902720).
Alternative names
Protein Wnt-3a, WNT3A
Recommended products
Rabbit Polyclonal WNT3A antibody. Suitable for IHC-P, WB and reacts with Mouse samples. Cited in 19 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Wnt3a protein also known as Wingless-related integration site 3a is a member of the Wnt family of proteins. It has a molecular mass of approximately 38 kDa. Wnt3a is a secreted glycoprotein that plays a mechanical role in cell signaling. It is expressed in various tissues including the neural tube somites and limb buds in the developing embryo. The protein functions by binding to Frizzled receptors on the cell surface initiating a signaling cascade that influences cellular behaviors.
- Biological function summary
Wnt3a plays a critical role in embryonic development and cell proliferation. As part of a larger complex it partners with other signaling molecules to ensure proper cellular communication. Wnt3a is important in patterning during embryogenesis influencing processes like axis formation and organogenesis. Its expression impacts the development of skeletal structures by regulating the differentiation and proliferation of precursor cells.
- Pathways
Wnt3a is an integral component of the canonical Wnt/β-catenin signaling pathway which regulates gene expression essential for cell fate decisions. This pathway is activated when Wnt3a binds to the Frizzled and LRP5/6 receptor complex stabilizing β-catenin in the cytosol. Glycogen synthase kinase 3 beta (GSK-3β) and Adenomatosis polyposis coli (APC) are key proteins involved which help mediate the signal to downstream targets involved in cell growth and development.
- Associated diseases and disorders
Wnt3a has significant associations with cancer and bone developmental disorders. Dysregulation of the Wnt3a signaling pathway can lead to tumorigenesis due to unchecked cellular proliferation and survival. In cancer components like β-catenin become targets for therapeutic interventions. In bone disorders improperly regulated Wnt3a expression can result in conditions like osteoporosis where the balance between bone formation and resorption is disrupted. FGFR2 a protein involved in skeletal abnormalities often interacts with dysregulated Wnt3a signaling highlighting its importance in bone health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody (ab19925)
IHC image of Wnt3a staining in mouse placenta formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19925, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times
Western blot - Anti-Wnt3a antibody (ab19925)
ab19925 detects a band of the expected size for recombinant mouse Wnt3a.
All lanes: Western blot - Anti-Wnt3a antibody (ab19925) at 1 µg/mL
All lanes: Recombinant mouse Wnt3a, AAs 19-352 with a 16 AA n-terminal non-Wnt3a sequence at 0.1 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG at 1/15000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa
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