Rabbit Polyclonal WNT3A antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 117 publications.
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- Oncology reports 51:2023Inhibitor of Wnt receptor 1 suppresses the effects of Wnt1, Wnt3a and β‑catenin on the proliferation and migration of C6 GSCs induced by low‑dose radiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Yan,Ying-Ying Cheng,Yan-Ru Li,Xu-Wen Jiao,Yin-Ming Liu,Hai-Yan Cai,Yin-Xiu DingPubMed 38099414
- Nature communications 14:19862023The IgG4 hinge with CD28 transmembrane domain improves VH-based CAR T cells targeting a membrane-distal epitope of GPC1 in pancreatic cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNan Li,Alex Quan,Dan Li,Jiajia Pan,Hua Ren,Gerard Hoeltzel,Natalia de Val,Dana Ashworth,Weiming Ni,Jing Zhou,Sean Mackay,Stephen M Hewitt,Raul Cachau,Mitchell HoPubMed 37031249
- BioMed research international 2022:86380852022Chalcone 9X Contributed to Repressing Glioma Cell Growth and Migration and Inducing Cell Apoptosis by Reducing FOXM1 Expression In Vitro and Repressing Tumor Growth In Vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenguang Li,Rui Wang,Wenshi Guo,Xu Feng,Ning GuanPubMed 35978634
- Arteriosclerosis, thrombosis, and vascular biology 42:1048-10592022Microcalcification and Thoracic Aortopathy: A Window Into Disease Severity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlexander J Fletcher,Jennifer Nash,Maaz B J Syed,Mark G Macaskill,Adriana A S Tavares,Niki Walker,Hannah Salcudean,Jonathon A Leipsic,Kelvin H H Lim,Jillian Madine,William Wallace,Mark Field,David E Newby,Rihab Bouchareb,Michael A Seidman,Riaz Akhtar,Stephanie L SellersPubMed 35770666
- Biology of reproduction 106:503-5142021Transcriptomic profiling of neonatal mouse granulosa cells reveals new insights into primordial follicle activation†.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEmmalee A Ford,Emily R Frost,Emma L Beckett,Shaun D Roman,Eileen A McLaughlin,Jessie M SutherlandPubMed 34673933
- International journal of stem cells 14:410-4222021p53 Promotes Differentiation of Cardiomyocytes from hiPSC through Wnt Signaling-Mediated Mesendodermal Differentiation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuanshu Liu,Peng Zhang,Wenjun Huang,Feng Liu,Dandan Long,Wanling Peng,Xitong Dang,Xiaorong Zeng,Rui ZhouPubMed 34158418
- PeerJ 9:e114552021Identification of KIF4A as a pan-cancer diagnostic and prognostic biomarker via bioinformatics analysis and validation in osteosarcoma cell lines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiankang Pan,Xiaohua Lei,Xinzhan MaoPubMed 34055488
- Pathology oncology research : POR 27:6096202021Wnt3a/β-Catenin/CBP Activation in the Progression of Cervical Intraepithelial Neoplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDingqing Feng,Jie Lin,Wenhui Wang,Keqin Yan,Haiyan Liang,Jing Liang,Huan Yu,Bin LingPubMed 34257574
- Neoplasma 68:557-5662021Paired-like homeodomain transcription factor 2 strengthens chemoresistance in colorectal cancer by activating the Wnt/β-catenin axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxian Shu,Chuan He,Yan Xiong,Jun Lan,Rongfeng SongPubMed 33780262
- International journal of nanomedicine 15:9211-92292020Neuroprotective Effects of Curcumin-Loaded Emulsomes in a Laser Axotomy-Induced CNS Injury Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElif Nur Yilmaz et. al.PubMed 33244233
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Expected | Not recommended | Tested |
| Mouse | Tested | Not recommended | Expected |
| Chicken | Predicted | Not recommended | Predicted |
| Xenopus laevis | Predicted | Not recommended | Predicted |
| Zebrafish | Predicted | Not recommended | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Chicken, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Xenopus laevis, Zebrafish | Dilution info - | Notes - |
Associated Products
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4 products for Alternative Product
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:20093360, PubMed:21244856, PubMed:24841207, PubMed:26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed:26902720).
Alternative names
Protein Wnt-3a, WNT3A
Recommended products
Rabbit Polyclonal WNT3A antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 117 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
Replenishment batches of our polyclonal antibody, ab28472 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab219412.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Wnt3a protein also known as Wingless-related integration site 3a is a member of the Wnt family of proteins. It has a molecular mass of approximately 38 kDa. Wnt3a is a secreted glycoprotein that plays a mechanical role in cell signaling. It is expressed in various tissues including the neural tube somites and limb buds in the developing embryo. The protein functions by binding to Frizzled receptors on the cell surface initiating a signaling cascade that influences cellular behaviors.
- Biological function summary
Wnt3a plays a critical role in embryonic development and cell proliferation. As part of a larger complex it partners with other signaling molecules to ensure proper cellular communication. Wnt3a is important in patterning during embryogenesis influencing processes like axis formation and organogenesis. Its expression impacts the development of skeletal structures by regulating the differentiation and proliferation of precursor cells.
- Pathways
Wnt3a is an integral component of the canonical Wnt/β-catenin signaling pathway which regulates gene expression essential for cell fate decisions. This pathway is activated when Wnt3a binds to the Frizzled and LRP5/6 receptor complex stabilizing β-catenin in the cytosol. Glycogen synthase kinase 3 beta (GSK-3β) and Adenomatosis polyposis coli (APC) are key proteins involved which help mediate the signal to downstream targets involved in cell growth and development.
- Associated diseases and disorders
Wnt3a has significant associations with cancer and bone developmental disorders. Dysregulation of the Wnt3a signaling pathway can lead to tumorigenesis due to unchecked cellular proliferation and survival. In cancer components like β-catenin become targets for therapeutic interventions. In bone disorders improperly regulated Wnt3a expression can result in conditions like osteoporosis where the balance between bone formation and resorption is disrupted. FGFR2 a protein involved in skeletal abnormalities often interacts with dysregulated Wnt3a signaling highlighting its importance in bone health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-Wnt3a antibody (ab28472)
ab28472 was tested against Mouse Wnt3a Full-length Recombinant Protein predicted to run at the molecular weight of 68kDa.
All lanes: Western blot - Anti-Wnt3a antibody (ab28472) at 1 µg/mL
All lanes: Mouse Wnt3a Full-length Recombinant Protein (Tagged) at 0.1 µg
SecondaryAll lanes: Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 39 kDa
Observed band size: 68 kDa
This image is courtesy of Ludovic Vallier, University of CambridgeImmunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody (ab28472)
ab28472 stained human embryonic stem cells differentiated into a heterogeneous mix of cells at 5 ug/ml.
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