AB28472

Anti-Wnt3a antibody

Name: Anti-Wnt3a antibody (ab28472) | Abcam
Brand: Abcam Limited
SKU: AB28472
Price: 480 USD
Availability: InStock
Rating: 3 (6 reviews)

(6 Reviews)

(138 Publications)

Rabbit Polyclonal WNT3A antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 138 publications.

View Alternative Names

Protein Wnt-3a, WNT3A

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody (AB28472)

ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody (AB28472)

ab28472 stained human embryonic stem cells differentiated into a heterogeneous mix of cells at 5 ug/ml.

This image is courtesy of Ludovic Vallier, University of Cambridge

Project

Western blot - Anti-Wnt3a antibody (AB28472)

ab28472 was tested against Mouse Wnt3a Full-length Recombinant Protein predicted to run at the molecular weight of 68kDa.

All lanes:

Western blot - Anti-Wnt3a antibody (ab28472) at 1 µg/mL

All lanes:

Mouse Wnt3a Full-length Recombinant Protein (Tagged) at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 39 kDa

Observed band size: 68 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Replenishment batches of our polyclonal antibody, ab28472 are tested in WB. Previous batches were additionally validated in ICC/IF. This application is still expected to work and is covered by our Abpromise guarantee. You may also be interested in our alternative recombinant antibody, ab219412.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Wnt3a protein also known as Wingless-related integration site 3a is a member of the Wnt family of proteins. It has a molecular mass of approximately 38 kDa. Wnt3a is a secreted glycoprotein that plays a mechanical role in cell signaling. It is expressed in various tissues including the neural tube somites and limb buds in the developing embryo. The protein functions by binding to Frizzled receptors on the cell surface initiating a signaling cascade that influences cellular behaviors.

Biological function summary

Wnt3a plays a critical role in embryonic development and cell proliferation. As part of a larger complex it partners with other signaling molecules to ensure proper cellular communication. Wnt3a is important in patterning during embryogenesis influencing processes like axis formation and organogenesis. Its expression impacts the development of skeletal structures by regulating the differentiation and proliferation of precursor cells.

Pathways

Wnt3a is an integral component of the canonical Wnt/β-catenin signaling pathway which regulates gene expression essential for cell fate decisions. This pathway is activated when Wnt3a binds to the Frizzled and LRP5/6 receptor complex stabilizing β-catenin in the cytosol. Glycogen synthase kinase 3 beta (GSK-3β) and Adenomatosis polyposis coli (APC) are key proteins involved which help mediate the signal to downstream targets involved in cell growth and development.

Wnt3a has significant associations with cancer and bone developmental disorders. Dysregulation of the Wnt3a signaling pathway can lead to tumorigenesis due to unchecked cellular proliferation and survival. In cancer components like β-catenin become targets for therapeutic interventions. In bone disorders improperly regulated Wnt3a expression can result in conditions like osteoporosis where the balance between bone formation and resorption is disrupted. FGFR2 a protein involved in skeletal abnormalities often interacts with dysregulated Wnt3a signaling highlighting its importance in bone health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed : 20093360, PubMed : 21244856, PubMed : 24841207, PubMed : 26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed : 26902720).

See full target information WNT3A

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Publications (138)

Recent publications for all applications. Explore the full list and refine your search

Diabetology & metabolic syndrome 17:378 PubMed41044791

2025

Electroacupuncture ameliorates cognitive dysfunction in T2DM rats by modulating astrocytic polarization and aberrant energy metabolism in the hippocampus via the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolu Li,Rui Li,Jiayi Lin,Xiaoli Wu

Histochemistry and cell biology 163:76 PubMed40658268

2025

Differentiation to insulin-positive cells from human amnion epithelial cells using a pancreatic development mimicry protocol.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Martínez-Rodríguez,Jonathan Salazar-Alonso,Axel Castro-Abrego,Daniela Ávila-González,Omar Martínez-Alarcón,Anayansi Molina-Hernández,Alejandro Martínez-Juárez,Héctor Salvador Godoy-Morales,Diana Elizabeth Lara-Barragán,Wendy Portillo,Nestor Emmanuel Díaz-Martínez,Guadalupe García-López,Nestor Fabián Díaz

International journal of molecular sciences 26: PubMed40430063

2025

Exploring the Bone-Liver Axis: Impact of Acute Ethanol Intoxication on Post-Traumatic Liver Inflammation and Damage Following Femur Fracture.

Applications

Unspecified application

Species

Unspecified reactive species

Jasmin Maria Bülow,Helen Rinderknecht,Nils Becker,Kernt Köhler,Alessa Wagner,Yuntao Yang,Katrin Bundkirchen,Claudia Neunaber,Borna Relja

Frontiers in physiology 16:1547661 PubMed40110183

2025

Cardiac implications of chicken wooden breast myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Thea Parsberg Støle,Andreas Romaine,Thea Kleiberg,Vibeke Høst,Marianne Lunde,Almira Hasic,Tiril Aurora Lintvedt,Karen Wahlstrøm Sanden,Svein O Kolset,Jens Petter Wold,Addolorata Pisconti,Sissel Beate Rønning,Cathrine Rein Carlson,Mona Elisabeth Pedersen

Scientific reports 15:4726 PubMed39922861

2025

Pax6 regulates neuronal migration and cell proliferation via interacting with Wnt3a during cortical development.

Applications

Unspecified application

Species

Unspecified reactive species

Bichao Zhang,Meihua Hou,Jiayan Huang,Yunfei Liu,Ciqing Yang,Juntang Lin

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 42:1933-1942 PubMed38520666

2024

Cardamonin inhibits osteogenic differentiation by downregulating Wnt/beta-catenin signaling and alleviates subchondral osteosclerosis in osteoarthritic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Fanding Meng,Pengchong Zhu,Xiaoli Ren,Limei Wang,Dong Ding,Jiangbo Yan,Ying Zhang,Shang-You Yang,Bin Ning

Environmental toxicology 39:3003-3013 PubMed38317500

2024

Baicalein targets STMN1 to inhibit the progression of nasopharyngeal carcinoma via regulating the Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Li,Xiaohang Cai

Environmental toxicology 39:2374-2389 PubMed38165020

2024

A transcriptomics-based investigation of the mechanism of pulmonary fibrosis induced by nickel oxide nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Han Liu,Mengmeng Yang,Kun Li,Qing Gao,Jinfa Zheng,Xuefeng Gong,Hui Wang,Yingbiao Sun,Xuhong Chang

Oncology reports 51: PubMed38099414

2023

Inhibitor of Wnt receptor 1 suppresses the effects of Wnt1, Wnt3a and β‑catenin on the proliferation and migration of C6 GSCs induced by low‑dose radiation.

Applications

Unspecified application

Species

Unspecified reactive species

Yu Yan,Ying-Ying Cheng,Yan-Ru Li,Xu-Wen Jiao,Yin-Ming Liu,Hai-Yan Cai,Yin-Xiu Ding

Neural regeneration research 19:1541-1547 PubMed38051897

2023

Activation of the Wnt/β-catenin/CYP1B1 pathway alleviates oxidative stress and protects the blood-brain barrier under cerebral ischemia/reperfusion conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Xingyong Chen,Nannan Yao,Yanguang Mao,Dongyun Xiao,Yiyi Huang,Xu Zhang,Yinzhou Wang

View all publications

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