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Anti-Wnt3a antibody [EPR21889] (ab219412) is a rabbit monoclonal antibody detecting Wnt3a in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.



- Biophysical QC for unrivalled batch-batch consistency

- Over 50 publications


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Images

Immunoprecipitation - Anti-Wnt3a antibody [EPR21889] (AB219412), expandable thumbnail
  • Western blot - Anti-Wnt3a antibody [EPR21889] (AB219412), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Wnt3a antibody [EPR21889] (AB219412), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody [EPR21889] (AB219412), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] (AB219412), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBICC/IFFlow Cyt (Intra)IHC-P
Human
Expected
Tested
Expected
Expected
Not recommended
Mouse
Tested
Tested
Tested
Tested
Tested
Rat
Expected
Not recommended
Expected
Expected
Tested

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/100
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

-

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

This antibody is not suitable for human species in IHC-P application.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

This antibody is not suitable for human species in IHC-P application.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

This antibody is not suitable for human species in IHC-P application.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt signaling pathway that results in activation of transcription factors of the TCF/LEF family (PubMed:20093360, PubMed:21244856, PubMed:24841207, PubMed:26902720). Required for normal embryonic mesoderm development and formation of caudal somites. Required for normal morphogenesis of the developing neural tube (By similarity). Mediates self-renewal of the stem cells at the bottom on intestinal crypts (in vitro) (PubMed:26902720).

Alternative names

Protein Wnt-3a, WNT3A

Recommended products

Anti-Wnt3a antibody [EPR21889] (ab219412) is a rabbit monoclonal antibody detecting Wnt3a in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.



- Biophysical QC for unrivalled batch-batch consistency

- Over 50 publications


Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR21889
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for human IHC-P. Rat species is recommended based on IHC result. We do not guarantee western blot for rat.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Wnt3a antibody [EPR21889] (ab219412) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Wnt3a?


Anti-Wnt3a [EPR21889] (ab219412) specifically detects a band for Wnt3a (UniProt: P56704) at a molecular weight of 39kDa.

Trusted by the scientific community


Anti-Wnt3a [EPR21889] (ab219412) was first used in a scientific publication in 2018 and has been cited over 50 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products


We have a range of other formats of antibody clone [EPR21889] also available for your convenience:
ab219412, Carrier free - Anti-Wnt3a antibody [EPR21889] - BSA and Azide free ab234099, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Wnt3a antibody [EPR21889] ab270093, PE - PE Anti-Wnt3a antibody [EPR21889] ab305728, APC - APC Anti-Wnt3a antibody [EPR21889] ab305729, HRP - HRP Anti-Wnt3a antibody [EPR21889] ab305730, Alkaline Phosphatase - Alkaline Phosphatase Anti-Wnt3a antibody [EPR21889] ab308719, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Wnt3a antibody [EPR21889] ab309684, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Wnt3a antibody [EPR21889] ab310437, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Wnt3a antibody [EPR21889] ab311964, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-Wnt3a antibody [EPR21889] ab312436, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-Wnt3a antibody [EPR21889] ab321404



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Wnt3a protein also known as Wingless-related integration site 3a is a member of the Wnt family of proteins. It has a molecular mass of approximately 38 kDa. Wnt3a is a secreted glycoprotein that plays a mechanical role in cell signaling. It is expressed in various tissues including the neural tube somites and limb buds in the developing embryo. The protein functions by binding to Frizzled receptors on the cell surface initiating a signaling cascade that influences cellular behaviors.

Biological function summary

Wnt3a plays a critical role in embryonic development and cell proliferation. As part of a larger complex it partners with other signaling molecules to ensure proper cellular communication. Wnt3a is important in patterning during embryogenesis influencing processes like axis formation and organogenesis. Its expression impacts the development of skeletal structures by regulating the differentiation and proliferation of precursor cells.

Pathways

Wnt3a is an integral component of the canonical Wnt/Β-catenin signaling pathway which regulates gene expression essential for cell fate decisions. This pathway is activated when Wnt3a binds to the Frizzled and LRP5/6 receptor complex stabilizing Β-catenin in the cytosol. Glycogen synthase kinase 3 beta (GSK-3Β) and Adenomatosis polyposis coli (APC) are key proteins involved which help mediate the signal to downstream targets involved in cell growth and development.

Associated diseases and disorders

Wnt3a has significant associations with cancer and bone developmental disorders. Dysregulation of the Wnt3a signaling pathway can lead to tumorigenesis due to unchecked cellular proliferation and survival. In cancer components like Β-catenin become targets for therapeutic interventions. In bone disorders improperly regulated Wnt3a expression can result in conditions like osteoporosis where the balance between bone formation and resorption is disrupted. FGFR2 a protein involved in skeletal abnormalities often interacts with dysregulated Wnt3a signaling highlighting its importance in bone health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Immunoprecipitation - Anti-Wnt3a antibody [EPR21889] (ab219412), expandable thumbnail

    Immunoprecipitation - Anti-Wnt3a antibody [EPR21889] (ab219412)

    Wnt3a was immunoprecipitated from 0.35 mg L Wnt-3a (Wnt-3a stably-transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) whole cell lysate with ab219412 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab219412 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/5000 dilution.
    Lane 1: L Wnt-3a (Wnt-3a stably-transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) whole cell lysate 10 μg (Input).
    Lane 2: ab219412 IP in L Wnt-3A whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab219412 in L Wnt-3a whole cell lysate.
    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 10 seconds.

    All lanes: Immunoprecipitation - Anti-Wnt3a antibody [EPR21889] (ab219412)

    Predicted band size: 39 kDa

  • Western blot - Anti-Wnt3a antibody [EPR21889] (ab219412), expandable thumbnail

    Western blot - Anti-Wnt3a antibody [EPR21889] (ab219412)

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Wnt3a antibody [EPR21889] (ab219412) at 1/1000 dilution

    Lane 1: Human fetal brain lysate at 10 µg

    Lane 2: Human fetal skin lysate at 10 µg

    Lane 3: HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa

    Exposure time: 3min

  • Flow Cytometry (Intracellular) - Anti-Wnt3a antibody [EPR21889] (ab219412), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Wnt3a antibody [EPR21889] (ab219412)

    Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized L Wnt-3a (Wnt-3a stably transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) cell line (right)labeling Wnt3a with ab219412 at 1/500 compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left). Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), at 1/2000 dilution was used as the secondary antibody.

    Two Wnt3a-expressing populations can be seen labeled in this stably transfected cell line.

  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody [EPR21889] (ab219412), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Wnt3a antibody [EPR21889] (ab219412)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized L Wnt-3a (Wnt-3a stably transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) cells labeling Wnt3a with ab219412 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining in L Wnt-3A cell line. The nuclear counter stain is DAPI (blue).
    Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at a 1/200 dilution (red).
    The negative control is the secondary antibody only.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] (ab219412), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] (ab219412)

    Immunohistochemical analysis of paraffin-embedded rat E14.5 tissue labeling Wnt3a with ab219412 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on the hem remnant of rat E14.5 (PMID: 15509764; PMID: 16410414) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] (ab219412), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt3a antibody [EPR21889] (ab219412)

    Immunohistochemical analysis of paraffin-embedded mouse E14.5 tissue labeling Wnt3a with ab219412 at 1/500 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining on the hem remnant of mouse E14.5 (PMID: 15509764; PMID: 16410414) is observed. Counter stained with Hematoxylin.
    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
    Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

  • Western blot - Anti-Wnt3a antibody [EPR21889] (ab219412), expandable thumbnail

    Western blot - Anti-Wnt3a antibody [EPR21889] (ab219412)

    Blocking/ Dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Western blot - Anti-Wnt3a antibody [EPR21889] (ab219412) at 1/20000 dilution

    Lane 1: His-tagged human Wnt3 recombinant protein (aa22-355), 40 ng

    Lane 2: His-tagged human Wnt3a recombinant protein (aa19-352), 20 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa

    Exposure time: 3min

  • Western blot - Anti-Wnt3a antibody [EPR21889] (ab219412), expandable thumbnail

    Western blot - Anti-Wnt3a antibody [EPR21889] (ab219412)

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    Exposure times: Lane 1: 3 minutes; Lane 2: 3 seconds.

    All lanes: Western blot - Anti-Wnt3a antibody [EPR21889] (ab219412) at 1/1000 dilution

    Lane 1: L Wnt-3a (Wnt-3a stably transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) supernatant at 20 µL

    Lane 2: L Wnt-3a (Wnt-3a stably transfected mouse subcutaneous connective tissue areolar and adipose fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 39 kDa

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Product protocols

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