AB277798

Anti-Wnt4 antibody [9HCLC]

Recombinant
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(3 Publications)

Rabbit Recombinant Multiclonal WNT4 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human WNT4 aa 50-100.

View Alternative Names

UNQ426/PRO864, WNT4, Protein Wnt-4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Wnt4 antibody [9HCLC] (AB277798)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Wnt4 antibody [9HCLC] (AB277798)

Immunofluorescence was performed on fixed and permeabilized HepG2 cells for detection of Wnt4 using Anti-Wnt4 Recombinant Rabbit Polyclonal Antibody (ab277798, 0.5 μg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal^™ Secondary Antibody, Alexa Fluor^® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of Wnt4 protein (green), Panel b) is stained for nuclei (blue) using SlowFade^® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor^® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of Wnt4 protein. Panel e) represents control cells with no primary Antibody to assess background.

Supplier Data

Western blot - Anti-Wnt4 antibody [9HCLC] (AB277798)

All lanes:

Western blot - Anti-Wnt4 antibody [9HCLC] (ab277798) at 1 µg/mL

Lane 1:

Jurkat whole cell extracts at 30 µg

Lane 2:

K562 whole cell extracts at 30 µg

Lane 3:

HCT 116 whole cell extracts at 30 µg

Lane 4:

PANC1 whole cell extracts at 30 µg

Lane 5:

HeLa whole cell extracts at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal HRP conjugate at 1/2500 dilution

Predicted band size: 39 kDa

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Supplier Data

Western blot - Anti-Wnt4 antibody [9HCLC] (AB277798)

All lanes:

Western blot - Anti-Wnt4 antibody [9HCLC] (ab277798) at 1 µg/mL

Lane 1:

A-431 cells untransfected

Lane 2:

A-431 cells transfected with non-specific scrambled siRNA

Lane 3:

A-431 whole cell extract with WNT4 knockdown

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Superclonal HRP conjugate at 1/4000 dilution

Predicted band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

9HCLC

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

This product was produced with the following immunogens:

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human WNT4 aa 250-300. Database link P56705

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human WNT4 aa 200-300. Database link P56705

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.5 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5-1 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

- The sensitivity of polyclonal antibodies by recognising multiple epitopes
- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt4 also known as Wingless-type MMTV integration site family member 4 is a protein that has significant role in cell signaling. With a molecular mass of approximately 39 kDa this protein is expressed in various tissues notably in mammalian systems such as human thyroid tissue. Wnt4 primarily facilitates communication between cells by activating signaling pathways that regulate development and differentiation processes. It is widely studied in different species including model organisms like the marmoset.

Biological function summary

Wnt4 is integral to embryonic development influencing the formation of organs and tissues. As part of the Wnt signaling pathway it works as a ligand interacting with Frizzled receptors on the cell surface. Wnt4 often in collaboration with other Wnt proteins contributes to cellular processes like proliferation migration and gene expression modulation. Its function during the development of reproductive systems and kidney morphogenesis highlights its importance in developmental biology.

Pathways

Wnt4 operates within the canonical and non-canonical Wnt signaling pathways. It primarily influences the beta-catenin signaling pathway essential for maintaining cellular homeostasis and regulating developmental signals. It can interact with other Wnt pathway components such as beta-catenin and GSK3 to modulate transcriptional activities important for cellular differentiation. By doing so it plays a pivotal role in coordinating complex signaling networks that oversee cell fate decisions.

Wnt4 displays notable associations with conditions such as Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome and polycystic ovary syndrome (PCOS). Both disorders relate to abnormalities in the reproductive system highlighting Wnt4's role in sexual development. Altered Wnt4 expression can affect other signaling proteins like beta-catenin contributing to disease pathology. By understanding its contribution to these disorders researchers aim to uncover potential therapeutic targets that rectify imbalances in Wnt signaling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung (PubMed : 15317892, PubMed : 16959810, PubMed : 18179883, PubMed : 18182450). Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development (By similarity). Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed : 15317892, PubMed : 16959810, PubMed : 18182450). Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed : 15317892, PubMed : 16959810, PubMed : 18182450). Required for normal lung development and for normal patterning of trachael cartilage rings (By similarity).

See full target information WNT4

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan 45:266-271 PubMed40151113

2025

Effects of Huluan decotion on cyclophosphamide-induced autoimmune premature ovarian failure in murine models.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Guiling,Zhou Xiaolin,Shen Chengwan,L I Panxiao,Abulizi Abudula

Experimental and therapeutic medicine 25:75 PubMed36684653

2023

Wnt4 prevents apoptosis and inflammation of dental pulp cells induced by LPS by inhibiting the IKK/NF‑κB pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chengli Ni,Gang Wu,Tingting Miao,Jianguang Xu

Oral diseases 29:1979-1990 PubMed35403775

2022

Circ_0001971 makes progress of oral squamous cell carcinoma by targeting miR-107/FZD4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaopeng Lu,Hongguo Xie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt4 antibody [9HCLC]

Immunocytochemistry/ Immunofluorescence - Anti-Wnt4 antibody [9HCLC] (AB277798)

Western blot - Anti-Wnt4 antibody [9HCLC] (AB277798)

Western blot - Anti-Wnt4 antibody [9HCLC] (AB277798)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise