Anti-Wnt4 antibody [EPR25073-14] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Wnt4 antibody [EPR25073-14] - BSA and Azide free (AB324816)

This data was developed using ab322901, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Wnt4 antibody[EPR25073-14] (<a href='/en-us/products/primary-antibodies/wnt4-antibodyepr25073-14-ab322901'>ab322901</a>) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

A431 transfected with siRNA specifically targeting WNT4 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,36 kDa

true

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-Wnt4 antibody [EPR25073-14] - BSA and Azide free (AB324816)

This data was developed using ab322901, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the higher MW band at approximately 80 kDa (in lane 1) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Wnt4 antibody[EPR25073-14] (<a href='/en-us/products/primary-antibodies/wnt4-antibodyepr25073-14-ab322901'>ab322901</a>) at 1/1000 dilution

Lane 1:

Human ovary cancer tissue lysate at 20 µg

Lane 2:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 40 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Wnt4 antibody [EPR25073-14] - BSA and Azide free (AB324816)

This data was developed using ab322901, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : heart, prostate, spleen (PMID : 17014734)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Wnt4 antibody[EPR25073-14] (<a href='/en-us/products/primary-antibodies/wnt4-antibodyepr25073-14-ab322901'>ab322901</a>) at 1/1000 dilution

Lane 1:

Mouse ovary tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse prostate tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat ovary tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,36 kDa

false

Exposure time: 180s

• Dot

Supplier Data

Dot Blot - Anti-Wnt4 antibody [EPR25073-14] - BSA and Azide free (AB324816)

This data was developed using ab322901, the same antibody clone in a different buffer formulation.

Dot blot analysis of Wnt4 using ab322901 at 1 : 1000 (0.5 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : His-tagged human Wnt4 recombinant fragment
Lane2 : His-tagged human Wnt2b recombinant fragment
Lane3 : His-tagged human Wnt3a recombinant fragment
Lane4 : LIF-tagged human Wnt5a recombinant fragment

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human Wnt2b, Wnt3a or Wnt5a.

In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
In Dot Blot, Anti-LIF antibody [EPR21713-25] - BSA and Azide free (Capture) (ab244795) staining at 1/1000 dilution.

All lanes:

Dot Blot - Anti-Wnt4 antibody[EPR25073-14] (<a href='/en-us/products/primary-antibodies/wnt4-antibodyepr25073-14-ab322901'>ab322901</a>) at 1/1000 dilution

Lane 1:

His-tagged human Wnt4 recombinant fragment

Lane 2:

His-tagged human Wnt2b recombinant fragment

Lane 3:

His-tagged human Wnt3a recombinant fragment

Lane 4:

LIF-tagged human Wnt5a recombinant fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s