AB317117

Anti-Wnt4 antibody [HL1889]

Name: Anti-Wnt4 antibody [HL1889] (ab317117) | Abcam
Brand: Abcam Limited
SKU: AB317117

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Rabbit Monoclonal WNT4 antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate, Zebrafish, Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human WNT4.

View Alternative Names

UNQ426/PRO864, WNT4, Protein Wnt-4

4 Images

Supplier Data

Western blot - Anti-Wnt4 antibody [HL1889] (AB317117)

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ab317117 diluted at 1 : 2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Wnt4 antibody [HL1889] (ab317117) at 1/2000 dilution

All lanes:

Mouse skin tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-Wnt4 antibody [HL1889] (AB317117)

Whole zebrafish extract (30 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317117 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-Wnt4 antibody [HL1889] (ab317117) at 1/1000 dilution

All lanes:

Whole zebrafish extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Supplier Data

Western blot - Anti-Wnt4 antibody [HL1889] (AB317117)

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE and the membrane was blotted with ab317117 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-Wnt4 antibody [HL1889] (ab317117) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts

Lane 2:

Wnt 1-transfected (+) 293T whole cell extracts

Lane 3:

Wnt 2-transfected (+) 293T whole cell extracts

Lane 4:

Wnt 2b-transfected (+) 293T whole cell extracts

Lane 5:

Wnt 3-transfected (+) 293T whole cell extracts

Lane 6:

Wnt 3a-transfected (+) 293T whole cell extracts

Lane 7:

Wnt 4-transfected (+) 293T whole cell extracts

Lane 8:

Wnt 5a-transfected (+) 293T whole cell extracts

Lane 9:

Wnt 6-transfected (+) 293T whole cell extracts

Lane 10:

Wnt 7a-transfected (+) 293T whole cell extracts

Lane 11:

Wnt 7b-transfected (+) 293T whole cell extracts

Lane 12:

Wnt 8a-transfected (+) 293T whole cell extracts

Lane 13:

Wnt 8b-transfected (+) 293T whole cell extracts

Lane 14:

Wnt 9a-transfected (+) 293T whole cell extracts

Lane 15:

Wnt 9b-transfected (+) 293T whole cell extracts

Lane 16:

Wnt 10a-transfected (+) 293T whole cell extracts

Lane 17:

Wnt 10b-transfected (+) 293T whole cell extracts

Lane 18:

Wnt 16-transfected (+) 293T whole cell extracts

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Supplier Data

Western blot - Anti-Wnt4 antibody [HL1889] (AB317117)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317117 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Wnt4 antibody [HL1889] (ab317117) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

V5-tagged Wnt4 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1889

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Zebrafish

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human WNT4.

P56705

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt4 also known as Wingless-type MMTV integration site family member 4 is a protein that has significant role in cell signaling. With a molecular mass of approximately 39 kDa this protein is expressed in various tissues notably in mammalian systems such as human thyroid tissue. Wnt4 primarily facilitates communication between cells by activating signaling pathways that regulate development and differentiation processes. It is widely studied in different species including model organisms like the marmoset.

Biological function summary

Wnt4 is integral to embryonic development influencing the formation of organs and tissues. As part of the Wnt signaling pathway it works as a ligand interacting with Frizzled receptors on the cell surface. Wnt4 often in collaboration with other Wnt proteins contributes to cellular processes like proliferation migration and gene expression modulation. Its function during the development of reproductive systems and kidney morphogenesis highlights its importance in developmental biology.

Pathways

Wnt4 operates within the canonical and non-canonical Wnt signaling pathways. It primarily influences the beta-catenin signaling pathway essential for maintaining cellular homeostasis and regulating developmental signals. It can interact with other Wnt pathway components such as beta-catenin and GSK3 to modulate transcriptional activities important for cellular differentiation. By doing so it plays a pivotal role in coordinating complex signaling networks that oversee cell fate decisions.

Wnt4 displays notable associations with conditions such as Mayer-Rokitansky-Küster-Hauser (MRKH) syndrome and polycystic ovary syndrome (PCOS). Both disorders relate to abnormalities in the reproductive system highlighting Wnt4's role in sexual development. Altered Wnt4 expression can affect other signaling proteins like beta-catenin contributing to disease pathology. By understanding its contribution to these disorders researchers aim to uncover potential therapeutic targets that rectify imbalances in Wnt signaling.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung (PubMed : 15317892, PubMed : 16959810, PubMed : 18179883, PubMed : 18182450). Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development (By similarity). Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed : 15317892, PubMed : 16959810, PubMed : 18182450). Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed : 15317892, PubMed : 16959810, PubMed : 18182450). Required for normal lung development and for normal patterning of trachael cartilage rings (By similarity).

See full target information WNT4

Additional targets

Wnt4,

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Product promise

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