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AB322901

Anti-Wnt4 antibody[EPR25073-14]

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Rabbit Recombinant Monoclonal WNT4 antibody. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

View Alternative Names

UNQ426/PRO864, WNT4, Protein Wnt-4

4 Images
Western blot - Anti-Wnt4 antibody[EPR25073-14] (AB322901)
  • WB

Supplier Data

Western blot - Anti-Wnt4 antibody[EPR25073-14] (AB322901)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

A431 transfected with siRNA specifically targeting WNT4 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,36 kDa

true

Exposure time: 81s

Western blot - Anti-Wnt4 antibody[EPR25073-14] (AB322901)
  • WB

Supplier Data

Western blot - Anti-Wnt4 antibody[EPR25073-14] (AB322901)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The identity of the higher MW band at approximately 80 kDa (in lane 1) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901) at 1/1000 dilution

Lane 1:

Human ovary cancer tissue lysate at 20 µg

Lane 2:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 40 kDa,36 kDa

false

Exposure time: 180s

Dot Blot - Anti-Wnt4 antibody[EPR25073-14] (AB322901)
  • Dot

Supplier Data

Dot Blot - Anti-Wnt4 antibody[EPR25073-14] (AB322901)

Dot blot analysis of Wnt4 using ab322901 at 1 : 1000 (0.5 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : His-tagged human Wnt4 recombinant fragment
Lane2 : His-tagged human Wnt2b recombinant fragment
Lane3 : His-tagged human Wnt3a recombinant fragment
Lane4 : LIF-tagged human Wnt5a recombinant fragment

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human Wnt2b, Wnt3a or Wnt5a.

In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
In Dot Blot, Anti-LIF antibody [EPR21713-25] - BSA and Azide free (Capture) (ab244795) staining at 1/1000 dilution.

All lanes:

Dot Blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901) at 1/1000 dilution

Lane 1:

His-tagged human Wnt4 recombinant fragment

Lane 2:

His-tagged human Wnt2b recombinant fragment

Lane 3:

His-tagged human Wnt3a recombinant fragment

Lane 4:

LIF-tagged human Wnt5a recombinant fragment

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

Western blot - Anti-Wnt4 antibody[EPR25073-14] (AB322901)
  • WB

Supplier Data

Western blot - Anti-Wnt4 antibody[EPR25073-14] (AB322901)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : heart, prostate, spleen (PMID : 17014734)

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901) at 1/1000 dilution

Lane 1:

Mouse ovary tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse prostate tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Rat ovary tissue lysate at 20 µg

Lane 6:

Rat heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 40 kDa,36 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-Wnt4 antibody [EPR25073-14] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25073-14

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB314820

Human Wnt4 ELISA Kit

Sandwich ELISA - Human Wnt4 ELISA Kit (AB314820)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung (PubMed : 15317892, PubMed : 16959810, PubMed : 18179883, PubMed : 18182450). Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development (By similarity). Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed : 15317892, PubMed : 16959810, PubMed : 18182450). Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed : 15317892, PubMed : 16959810, PubMed : 18182450). Required for normal lung development and for normal patterning of trachael cartilage rings (By similarity).
See full target information WNT4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com