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Rabbit Recombinant Monoclonal WNT4 antibody. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

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Images

Dot Blot - Anti-Wnt4 antibody[EPR25073-14] (AB322901), expandable thumbnail
  • Western blot - Anti-Wnt4 antibody[EPR25073-14] (AB322901), expandable thumbnail
  • Western blot - Anti-Wnt4 antibody[EPR25073-14] (AB322901), expandable thumbnail
  • Western blot - Anti-Wnt4 antibody[EPR25073-14] (AB322901), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
DotWBIHC-P
Human
Expected
Tested
Not recommended
Mouse
Expected
Tested
Not recommended
Rat
Expected
Tested
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended

Tested
Tested

Species

Recombinant fragment - Human

Dilution info

1/1000

Notes

-

Expected
Expected

Species

Human, Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/1000

Notes

-

Species

Mouse

Dilution info

1/1000

Notes

-

Species

Rat

Dilution info

1/1000

Notes

-

Not recommended
Not recommended

Species

Recombinant fragment - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Recombinant fragment - Human

Dilution info

-

Notes

-

Target data

Function

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Plays an important role in the embryonic development of the urogenital tract and the lung (PubMed:15317892, PubMed:16959810, PubMed:18179883, PubMed:18182450). Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development (By similarity). Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries (PubMed:15317892, PubMed:16959810, PubMed:18182450). Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary (PubMed:15317892, PubMed:16959810, PubMed:18182450). Required for normal lung development and for normal patterning of trachael cartilage rings (By similarity).

Alternative names

Rabbit Recombinant Monoclonal WNT4 antibody. Suitable for Dot, WB and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR25073-14

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Dot Blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901), expandable thumbnail

    Dot Blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901)

    Dot blot analysis of Wnt4 using ab322901 at 1:1000 (0.5 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

    Lane1: His-tagged human Wnt4 recombinant fragment

    Lane2: His-tagged human Wnt2b recombinant fragment

    Lane3: His-tagged human Wnt3a recombinant fragment

    Lane4: LIF-tagged human Wnt5a recombinant fragment

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This antibody does not cross-react with human Wnt2b, Wnt3a or Wnt5a.

    In Dot Blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    In Dot Blot, Anti-LIF antibody [EPR21713-25] - BSA and Azide free (Capture) (Anti-LIF antibody [EPR21713-25] - BSA and Azide free (Capture) ab244795) staining at 1/1000 dilution.

    All lanes: Dot Blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901) at 1/1000 dilution

    Lane 1: His-tagged human Wnt4 recombinant fragment

    Lane 2: His-tagged human Wnt2b recombinant fragment

    Lane 3: His-tagged human Wnt3a recombinant fragment

    Lane 4: LIF-tagged human Wnt5a recombinant fragment

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Exposure time: 180s

  • Western blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901), expandable thumbnail

    Western blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The identity of the higher MW band at approximately 80 kDa (in lane 1) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901) at 1/1000 dilution

    Lane 1: Human ovary cancer tissue lysate at 20 µg

    Lane 2: Human testis tissue lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 40 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901), expandable thumbnail

    Western blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: heart, prostate, spleen(PMID: 17014734)

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901) at 1/1000 dilution

    Lane 1: Mouse ovary tissue lysate at 20 µg

    Lane 2: Mouse heart tissue lysate at 20 µg

    Lane 3: Mouse prostate tissue lysate at 20 µg

    Lane 4: Mouse spleen tissue lysate at 20 µg

    Lane 5: Rat ovary tissue lysate at 20 µg

    Lane 6: Rat heart tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 40 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901), expandable thumbnail

    Western blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-Wnt4 antibody[EPR25073-14] (ab322901) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting WNT4 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 40 kDa, 36 kDa

    Exposure time: 81s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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