AB227229

Anti-Wnt5a antibody

Name: Anti-Wnt5a antibody (ab227229) | Abcam
Brand: Abcam Limited
SKU: AB227229

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(7 Publications)

Rabbit Polyclonal WNT5A antibody. Suitable for IHC-P, WB and reacts with Rat, Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WNT5A.

View Alternative Names

Protein Wnt-5a, WNT5A

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5a antibody (AB227229)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5a antibody (AB227229)

Immunohistochemical analysis of paraffin-embedded rat heart tissue stained for secreted Wnt5a protein with ab227229 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.

Supplier Data

Western blot - Anti-Wnt5a antibody (AB227229)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Wnt5a antibody (ab227229) at 1/1000 dilution

All lanes:

Panc-1 Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 42 kDa

false

Supplier Data

Western blot - Anti-Wnt5a antibody (AB227229)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-Wnt5a antibody (ab227229) at 1/500 dilution

All lanes:

Rat heart extract at 50 µg

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human WNT5A. The exact immunogen used to generate this antibody is proprietary information.

P41221

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/10000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt5a also known simply as Wnt-5a is a member of the Wnt family of signaling proteins. It plays a role in non-canonical Wnt signaling which means it does not involve the typical Wnt/beta-catenin pathway. The protein has a molecular weight of approximately 42 kDa and is widely expressed in various tissues including the brain lungs and reproductive organs. Wnt5a influences several cellular processes such as cell movement polarity and organ development by binding to Frizzled family receptors and co-receptors like ROR1 and ROR2.

Biological function summary

Wnt5a drives critical processes in development and tissue homeostasis. It acts by guiding cell migration and establishing cell polarity. Wnt5a is not part of a single large complex but interacts with membrane receptors and co-receptors to exert its effects. This interaction initiates signaling cascades that regulate cytoskeletal dynamics which is essential in both embryonic development and adult tissue maintenance. Wnt5a's role is pivotal in maintaining cellular organization and function across different biological contexts.

Pathways

Wnt5a participates significantly in the planar cell polarity pathway and the Wnt/Ca2+ signaling pathway. In these pathways Wnt5a affects the activity of proteins such as Dishevelled and CaMKII to influence cellular functions independently from beta-catenin. These pathways regulate processes like tissue morphogenesis and cell orientation maintaining the spatial arrangement and structural integrity of tissues.

Wnt5a has been linked to cancer progression and inflammatory diseases such as rheumatoid arthritis. It interacts with proteins like ROR1 and ROR2 during these pathologies. In cancer abnormal Wnt5a signaling can lead to enhanced cell migration and invasion contributing to metastasis. In rheumatoid arthritis Wnt5a may drive inflammatory pathways and joint tissue damage. Researchers consider Wnt5a a target for therapeutic interventions in these conditions with ongoing studies focused on modulating its activity for disease management.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed : 15735754). Mediates motility of melanoma cells (PubMed : 17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).

See full target information WNT5A

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 16:1534118 PubMed40365308

2025

Triptolide alleviates psoriasis through inhibiting the Wnt5a/β-Catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Eryang Chen,Lei Wang,Qu Wang,Yan Cai,Yaning Dou,Hongyan Qu,Junyi Zhu,Haiyang Zhao,Suqing Zheng,Chengguang Zhao,Bin Chen

Journal of orthopaedic translation 50:144-157 PubMed40171108

2025

Moderate static magnetic field regulates iron metabolism and salvage bone loss caused by iron accumulation.

Applications

Unspecified application

Species

Unspecified reactive species

Chenxiao Zhen,Shenghang Wang,Jiancheng Yang,Gejing Zhang,Chao Cai,Jianping Wang,Aifei Wang,Youjia Xu,Yanwen Fang,Min Wei,Dachuan Yin,Xinle Luo,Ming Gong,Hao Zhang,Peng Shang

Iranian journal of basic medical sciences 26:812-819 PubMed37396938

2023

MiR26-5p inhibits pathological pulmonary microvascular angiogenesis via down-regulating WNT5A.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Chen,Feng Gao,Dan Li,Jinquan Wang

Open life sciences 17:1591-1599 PubMed36518887

2022

PHF8 promotes osteogenic differentiation of BMSCs in old rat with osteoporosis by regulating Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Pan,Kai Huang,Hongbin Dai,Chunhe Sha

Molecular cell 82:4018-4032.e9 PubMed36332605

2022

LncRNA CCTT-mediated RNA-DNA and RNA-protein interactions facilitate the recruitment of CENP-C to centromeric DNA during kinetochore assembly.

Applications

Unspecified application

Species

Unspecified reactive species

Chong Zhang,Dongpeng Wang,Yajing Hao,Shuheng Wu,Jianjun Luo,Yuanchao Xue,Di Wang,Guohong Li,Lihui Liu,Changwei Shao,Huiyan Li,Jinfeng Yuan,Maoxiang Zhu,Xiang-Dong Fu,Xiao Yang,Runsheng Chen,Yan Teng

International journal of experimental pathology : PubMed33350543

2020

Circular RNA ITCH mediates HO-induced myocardial cell apoptosis by targeting miR-17-5p via wnt/β-catenin signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Nengfeng Zhang,Xu Wang

Gene 761:145038 PubMed32777532

2020

Downregulated circular RNA zRANB1 mediates Wnt5a/β-Catenin signaling to promote neuropathic pain via miR-24-3p/LPAR3 axis in CCI rat models.

Applications

Unspecified application

Species

Unspecified reactive species

Meng Wei,Lin Li,Yang Zhang,Min Zhang,Zhen Su

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Wnt5a antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5a antibody (AB227229)

Western blot - Anti-Wnt5a antibody (AB227229)

Western blot - Anti-Wnt5a antibody (AB227229)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise