Rabbit Polyclonal WNT5A antibody. Suitable for WB, IHC-P, ELISA and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human WNT5A.
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- Scientific reports 14:5592024WNTA5-mediated miR-374a-5p regulates vascular smooth muscle cell phenotype transformation and M1 macrophage polarization impacting intracranial aneurysm progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZengshi Li,Junqiang Huang,Lijian Yang,Xi Li,Wei LiPubMed 38177414
- Cell death discovery 9:3072023Haspin balances the ratio of asymmetric cell division through Wnt5a and regulates cell fate decisions in mouse embryonic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYingying Gao,Bin Ma,Yifan Li,Xiangyu Wu,Shifeng Zhao,Huiping Guo,Yiwei Wang,Lihua Sun,Jing XiePubMed 37612272
- Medicine 102:e338342023Aberrant expression of UBE2C in endometrial cancer and its correlation to epithelial mesenchymal transition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Zhang,Xueting Li,Yingying Gong,Danli Du,Huilei Chen,Lei Liu,Zenong ChengPubMed 37335710
- Science advances 9:eadd89772023Single-cell dissection of cellular and molecular features underlying human cervical squamous cell carcinoma initiation and progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChao Liu,Min Zhang,Xinlong Yan,Yanli Ni,Yandong Gong,Cong Wang,Xiaoling Zhang,Lingfei Wan,Hui Yang,Chen Ge,Yunqiao Li,Wenxue Zou,Rui Huang,Xiaohui Li,Bing Sun,Bing Liu,Jinbo Yue,Jinming YuPubMed 36706185
- Cancers 14:2022lncRNAs UC.145 and PRKG1-AS1 Determine the Functional Output of DKK1 in Regulating the Wnt Signaling Pathway in Gastric Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJung-Ho Yoon,Hyojoo Byun,Cristina Ivan,George A Calin,Dahyun Jung,Sangkil LeePubMed 35625973
- BioFactors (Oxford, England) 49:79-892020Circular RNA-0072309 has antitumor influences in Hep3B cell line by targeting microRNA-665.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQiuyun Yu,Jinhua Dai,Ming ShuPubMed 32048412
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human WNT5A. Database link P41221
Reactivity data
Select an application| WB | IHC-P | ICC/IF | ELISA | |
|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/5000.00000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed:15735754). Mediates motility of melanoma cells (PubMed:17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).
Alternative names
Protein Wnt-5a, WNT5A
Recommended products
Rabbit Polyclonal WNT5A antibody. Suitable for WB, IHC-P, ELISA and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human WNT5A.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.03% Proclin 300
Constituents: PBS, 50% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human WNT5A. Database link P41221
- Purification technique
- Affinity purification
- Concentration
- Purification notes
Purity >95%
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt5a also known simply as Wnt-5a is a member of the Wnt family of signaling proteins. It plays a role in non-canonical Wnt signaling which means it does not involve the typical Wnt/beta-catenin pathway. The protein has a molecular weight of approximately 42 kDa and is widely expressed in various tissues including the brain lungs and reproductive organs. Wnt5a influences several cellular processes such as cell movement polarity and organ development by binding to Frizzled family receptors and co-receptors like ROR1 and ROR2.
- Biological function summary
Wnt5a drives critical processes in development and tissue homeostasis. It acts by guiding cell migration and establishing cell polarity. Wnt5a is not part of a single large complex but interacts with membrane receptors and co-receptors to exert its effects. This interaction initiates signaling cascades that regulate cytoskeletal dynamics which is essential in both embryonic development and adult tissue maintenance. Wnt5a's role is pivotal in maintaining cellular organization and function across different biological contexts.
- Pathways
Wnt5a participates significantly in the planar cell polarity pathway and the Wnt/Ca2+ signaling pathway. In these pathways Wnt5a affects the activity of proteins such as Dishevelled and CaMKII to influence cellular functions independently from beta-catenin. These pathways regulate processes like tissue morphogenesis and cell orientation maintaining the spatial arrangement and structural integrity of tissues.
- Associated diseases and disorders
Wnt5a has been linked to cancer progression and inflammatory diseases such as rheumatoid arthritis. It interacts with proteins like ROR1 and ROR2 during these pathologies. In cancer abnormal Wnt5a signaling can lead to enhanced cell migration and invasion contributing to metastasis. In rheumatoid arthritis Wnt5a may drive inflammatory pathways and joint tissue damage. Researchers consider Wnt5a a target for therapeutic interventions in these conditions with ongoing studies focused on modulating its activity for disease management.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5a antibody (ab235966)
Paraffin-embedded human lung cancer tissue stained for Wnt5a using ab235966 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5a antibody (ab235966)
Paraffin-embedded human tonsil tissue stained for Wnt5a using ab235966 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5a antibody (ab235966)
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue using ab235966 at 1/67 dilution performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Western blot - Anti-Wnt5a antibody (ab235966)
All lanes: Western blot - Anti-Wnt5a antibody (ab235966) at 1/500 dilution
All lanes: HeLa whole cell lysate
SecondaryAll lanes: Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 42 kDa
Observed band size: 42 kDa
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