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AB325532

Anti-Wnt5a antibody [EPR29464-509]

  • RabMAb
  • Recombinant
  • 20ul selling size
  • KO Validated
  • What is this?

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Rabbit Recombinant Monoclonal WNT5A antibody. Suitable for WB, I-ELISA and reacts with Recombinant fragment - Human, Human, Mouse, Recombinant full length protein - Human samples.

View Alternative Names

Protein Wnt-5a, WNT5A

4 Images
Western blot - Anti-Wnt5a antibody [EPR29464-509] (AB325532)
  • WB

Lab

Western blot - Anti-Wnt5a antibody [EPR29464-509] (AB325532)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab325532 was shown to bind specifically to Wnt5a. Target of interest was observed at 42 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in WNT5A knockout cell line (lane 2) (lane 2, knockout cell line ab264019 / knockout cell lysate ab264515).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Wnt5a antibody [EPR29464-509] (ab325532) at 1/1000 dilution

Lane 1:

WI-38 (human fetal lung fibroblast) whole cell lysate at 80 µg

Lane 2:

Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 80 µg

Lane 3:

WNT5A knockout HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 42 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-Wnt5a antibody [EPR29464-509] (AB325532)
  • WB

Lab

Western blot - Anti-Wnt5a antibody [EPR29464-509] (AB325532)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : EL4.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Wnt5a antibody [EPR29464-509] (ab325532) at 1/1000 dilution

Lane 1:

L Wnt-5A (mouse Subcutaneous connective tissue) whole cell lysate at 80 µg

Lane 2:

EL4 (mouse lymphoma T lymphocyte) whole cell lysate at 80 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 42 kDa,36 kDa

false

Exposure time: 180s

Indirect ELISA - Anti-Wnt5a antibody [EPR29464-509] (AB325532)
  • I-ELISA

Lab

Indirect ELISA - Anti-Wnt5a antibody [EPR29464-509] (AB325532)

Indirect ELISA analysis of ab325532 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human Wnt5a protein; Human Wnt5b protein.

Antigen concentration : 1000 ng/ml

Western blot - Anti-Wnt5a antibody [EPR29464-509] (AB325532)
  • WB

Lab

Western blot - Anti-Wnt5a antibody [EPR29464-509] (AB325532)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human Wnt-5b.

In Western blot, Anti-Wnt-5a/b (ab325179) (1 : 1000) (42KDa).

All lanes:

Western blot - Anti-Wnt5a antibody [EPR29464-509] (ab325532) at 1/1000 dilution

Lane 1:

Non-tagged human Wnt-5a fragment at 10 ng

Lane 2:

Non-tagged human Wnt-5b fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 42 kDa

false

Exposure time: 48s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29464-509

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

WB, I-ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

For detection of endogenous Wnt5a in WI-38 and HeLa cells we loaded 80ug of total lysate samples.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Recombinant full length protein - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "250 ng/mL", "IELISA-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Can activate or inhibit canonical Wnt signaling, depending on receptor context. In the presence of FZD4, activates beta-catenin signaling. In the presence of ROR2, inhibits the canonical Wnt pathway by promoting beta-catenin degradation through a GSK3-independent pathway which involves down-regulation of beta-catenin-induced reporter gene expression (By similarity). Suppression of the canonical pathway allows chondrogenesis to occur and inhibits tumor formation. Stimulates cell migration. Decreases proliferation, migration, invasiveness and clonogenicity of carcinoma cells and may act as a tumor suppressor (PubMed : 15735754). Mediates motility of melanoma cells (PubMed : 17426020). Required during embryogenesis for extension of the primary anterior-posterior axis and for outgrowth of limbs and the genital tubercle. Inhibits type II collagen expression in chondrocytes (By similarity).
See full target information WNT5A
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com