Rabbit Polyclonal WNT5B antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Protein Wnt-5b.
Images
Publications
Filter by
- The Journal of biological chemistry 293:16528-165452018The NFκB subunit RELA is a master transcriptional regulator of the committed epithelial-mesenchymal transition in airway epithelial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBing Tian et. al.PubMed 30166344
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Protein Wnt-5b. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H1J7
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Common marmoset | Predicted |
| Dog | Predicted |
| Elephant | Predicted |
| Gorilla | Predicted |
| Horse | Predicted |
| Monkey | Predicted |
| Opossum | Predicted |
| Orangutan | Predicted |
| Pig | Predicted |
| Rabbit | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.5 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Horse, Dog, Pig, Monkey, Gorilla, Common marmoset, Orangutan, Elephant, Opossum | Dilution info - | Notes - |
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters (By similarity).
Alternative names
Protein Wnt-5b, WNT5B
Recommended products
Rabbit Polyclonal WNT5B antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Protein Wnt-5b.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Protein Wnt-5b. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H1J7
- Purification technique
- Affinity purification Immunogen
- Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except WNT5A (71%).
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt5b also known as wingless-type MMTV integration site family member 5B is a protein that is part of the Wnt family. It has a mass of approximately 42 kilodaltons. Wnt5b is expressed in various tissues notably in marmoset and human prostate tissue. It acts as a signaling molecule involving in cell communication processes such as cell fate determination proliferation and migration.
- Biological function summary
Wnt5b is involved in the regulation of embryonic development and is part of a non-canonical Wnt signaling pathway. It does not form a direct signaling complex but interacts with other proteins to execute its function. Wnt5b has a role in the modulation of cell polarity and movement being important for maintaining the structural integrity of tissues and the developmental processes.
- Pathways
Wnt5b plays a role in the planar cell polarity pathway and the Wnt/Ca2+ pathway. It connects to proteins like Dishevelled and Calcium/Calmodulin-dependent protein kinase II (CaMKII) acting to regulate intracellular calcium levels and influence cytoskeletal rearrangement. These pathways are critical for several aspects of cellular function including embryogenesis and tissue maintenance.
- Associated diseases and disorders
Wnt5b is associated with the development and progression of certain cancers including prostate cancer. Changes in Wnt5b signaling can disrupt cell-to-cell communication and lead to tumor growth. Additionally links exist between Wnt5b and other proteins such as beta-catenin in the context of cancer. Understanding these connections can offer insights into potential therapeutic strategies aimed at modulating Wnt5b activity to treat disease.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5b antibody (ab150602)
Immunohistochemical analysis of formalin-fixed paraffin embedded Human prostate (following heat-induced antigen retrieval) labelling Wnt5b using ab150602 at 2.5 μg/ml .
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com