Rabbit Polyclonal WNT6 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 11 publications.
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- Medical oncology (Northwood, London, England) 39:152021ELK1-induced up-regulation of KIF26B promotes cell cycle progression in breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSuXia Wang,Hui Zhang,HaiTing Liu,XiangYu Guo,RanRan Ma,WenJie Zhu,P GaoPubMed 34817735
- The Journal of clinical investigation 131:2021WNT6/ACC2-induced storage of triacylglycerols in macrophages is exploited by Mycobacterium tuberculosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJulius Brandenburg,Sebastian Marwitz,Simone C Tazoll,Franziska Waldow,Barbara Kalsdorf,Tim Vierbuchen,Thomas Scholzen,Annette Gross,Svenja Goldenbaum,Alexandra Hölscher,Martina Hein,Lara Linnemann,Maja Reimann,Andreas Kispert,Michael Leitges,Jan Rupp,Christoph Lange,Stefan Niemann,Jochen Behrends,Torsten Goldmann,Holger Heine,Ulrich E Schaible,Christoph Hölscher,Dominik Schwudke,Norbert ReilingPubMed 34255743
- Molecular oncology 14:1224-12412020A novel molecular link between HOXA9 and WNT6 in glioblastoma identifies a subgroup of patients with particular poor prognosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCéline S Gonçalves et. al.PubMed 31923345
- Journal of Cancer 10:2619-26272019High WNT6 expression indicates unfavorable survival outcome for patients with colorectal liver metastasis after liver resection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianhong Peng et. al.PubMed 31258769
- Theranostics 8:4805-48232018is a novel oncogenic prognostic biomarker in human glioblastoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCéline S Gonçalves et. al.PubMed 30279739
- Oncology letters 16:1163-11722018Wnt6 contributes tumorigenesis and development of colon cancer via its effects on cell proliferation, apoptosis, cell-cycle and migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiao-Li Zheng et. al.PubMed 29963191
- PloS one 12:e01875572017The role of H19, a long non-coding RNA, in mouse liver postnatal maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChad Pope et. al.PubMed 29099871
- International journal of clinical and experimental 8:11421-72015Increased WNT6 expression in tumor cells predicts unfavorable survival in esophageal squamous cell carcinoma patients.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLin Zhang et. al.PubMed 26617869
- PloS one 9:e921592014Porcupine is not required for the production of the majority of Wnts from primary human astrocytes and CD8+ T cells.Applications:WBReactive species:Unspecified reactive speciesMaureen H Richards et. al.PubMed 24647048
- Oncogene 32:375-872012WNT6 is a novel target gene of caveolin-1 promoting chemoresistance to epirubicin in human gastric cancer cells.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
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Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Together with CAV1 may promote chemoresistance of gastric cancer cells to DNA-damaging anthracycline drugs through the activation of the canonical Wnt receptor signaling pathway.
Alternative names
Protein Wnt-6, WNT6
Recommended products
Rabbit Polyclonal WNT6 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 11 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt6 also known simply as Wnt-6 is a member of the Wnt protein family characterized by its role in signaling pathways that regulate cell fate and patterning. It weighs approximately 50 kDa and is often found expressed in various tissues including the epidermis central nervous system and developing limbs. Wnt6 interacts with frizzled receptors on the cell surface to transmit signals that influence key cellular processes. Though the precise expression can vary it is generally consistent in tissues involved in development and repair.
- Biological function summary
Wnt6 plays a significant role in embryonic development and tissue regeneration. By participating in the Wnt signaling pathways it helps in the control of cell division migration and differentiation. Wnt6 does not function alone but is part of a larger complex that includes other Wnt family members and receptors which collaborate to ensure proper signaling and cellular responses. Its activity is important during critical periods of development influencing the formation of various organs and tissues.
- Pathways
Wnt6 is an important player in the canonical Wnt/β-catenin signaling pathway. This pathway is instrumental in regulating gene expression that determines cell behavior. Additionally Wnt6 is part of the planar cell polarity pathway interacting with other Wnt proteins like Wnt3a and signaling molecules such as Dishevelled and β-catenin. These pathways collectively regulate important cellular processes and maintain proper tissue architecture and functionality through coordinated signaling.
- Associated diseases and disorders
Wnt6 has been linked to various cancer types and developmental abnormalities. Its dysregulation can contribute to carcinogenesis particularly in breast cancer where altered Wnt6 expression impacts cellular proliferation and survival. Additionally its role in limb development suggests that mutations or misregulation might lead to limb deformities or skeletal disorders. Aberrant Wnt6 activity often correlates with changes in associated proteins such as β-catenin and frizzled receptors which together can offer potential targets for therapeutic intervention.
Product promise
Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Wnt6 antibody (ab50030)
Wnt6 has a predicted molecular weight of 40 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). Abcam currently sell two antibodies to this target, ab49946 which detects a band at 78 kDa and ab50030 which detects a band at 55 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
All lanes: Western blot - Anti-Wnt6 antibody (ab50030) at 1 µg/mL
Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 2: Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3: A431 (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 4: HEK293 Human embryonic kidney cell line Whole Cell Lysate at 10 µg
Lane 5: HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Lane 6: MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg
Lane 7: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg
Lane 8: U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 40 kDa
Observed band size: 55 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Wnt6 antibody (ab50030)
ICC/IF image of ab50030 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab50030, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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