AB154144

Anti-Wnt6 antibody [EPR9244]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal WNT6 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 4 publications.

View Alternative Names

Protein Wnt-6, WNT6

1 Images

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Western blot - Anti-Wnt6 antibody [EPR9244] (AB154144)

All lanes:

Western blot - Anti-Wnt6 antibody [EPR9244] (ab154144) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

SH-SY5Y cell lysate at 10 µg

Lane 4:

C6 cell lysate at 10 µg

Lane 5:

RAW 264.7 cell lysate at 10 µg

Lane 6:

PC12 cell lysate at 10 µg

Lane 7:

NIH 3T3 cell lysate at 10 µg

Lane 8:

Neuro 2a cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 40 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9244

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt6 also known simply as Wnt-6 is a member of the Wnt protein family characterized by its role in signaling pathways that regulate cell fate and patterning. It weighs approximately 50 kDa and is often found expressed in various tissues including the epidermis central nervous system and developing limbs. Wnt6 interacts with frizzled receptors on the cell surface to transmit signals that influence key cellular processes. Though the precise expression can vary it is generally consistent in tissues involved in development and repair.

Biological function summary

Wnt6 plays a significant role in embryonic development and tissue regeneration. By participating in the Wnt signaling pathways it helps in the control of cell division migration and differentiation. Wnt6 does not function alone but is part of a larger complex that includes other Wnt family members and receptors which collaborate to ensure proper signaling and cellular responses. Its activity is important during critical periods of development influencing the formation of various organs and tissues.

Pathways

Wnt6 is an important player in the canonical Wnt/β-catenin signaling pathway. This pathway is instrumental in regulating gene expression that determines cell behavior. Additionally Wnt6 is part of the planar cell polarity pathway interacting with other Wnt proteins like Wnt3a and signaling molecules such as Dishevelled and β-catenin. These pathways collectively regulate important cellular processes and maintain proper tissue architecture and functionality through coordinated signaling.

Wnt6 has been linked to various cancer types and developmental abnormalities. Its dysregulation can contribute to carcinogenesis particularly in breast cancer where altered Wnt6 expression impacts cellular proliferation and survival. Additionally its role in limb development suggests that mutations or misregulation might lead to limb deformities or skeletal disorders. Aberrant Wnt6 activity often correlates with changes in associated proteins such as β-catenin and frizzled receptors which together can offer potential targets for therapeutic intervention.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Together with CAV1 may promote chemoresistance of gastric cancer cells to DNA-damaging anthracycline drugs through the activation of the canonical Wnt receptor signaling pathway.

See full target information WNT6

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22776 PubMed36688817

2023

Loss of aquaporin 5 contributes to the corneal epithelial pathogenesis via Wnt/β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yihui Wang,Guohu Di,Kaier Zhang,Ying Bai,Xin Cao,Hui Zhao,Dianqiang Wang,Peng Chen

Veterinary sciences 8: PubMed34941843

2021

Roles of WNT6 in Sheep Endometrial Epithelial Cell Cycle Progression and Uterine Glands Organogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoxiao Gao,Xiaolei Yao,Xiaodan Li,Yaxu Liang,Zifei Liu,Zhibo Wang,Kang Li,Yingqi Li,Guomin Zhang,Feng Wang

Theranostics 8:4805-4823 PubMed30279739

2018

is a novel oncogenic prognostic biomarker in human glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Céline S Gonçalves,Joana Vieira de Castro,Marta Pojo,Eduarda P Martins,Sandro Queirós,Emmanuel Chautard,Ricardo Taipa,Manuel Melo Pires,Afonso A Pinto,Fernando Pardal,Carlos Custódia,Cláudia C Faria,Carlos Clara,Rui M Reis,Nuno Sousa,Bruno M Costa

Experimental and therapeutic medicine 14:5339-5344 PubMed29285061

2017

Wnt6 influences the viability of mouse embryonic palatal mesenchymal cells via the β-catenin pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Jiang,Lin Pan,Xiaoling Chen,Zhiqun Chen,Dongwei Xu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt6 antibody [EPR9244]

Western blot - Anti-Wnt6 antibody [EPR9244] (AB154144)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise