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AB317091

Anti-Wnt6 antibody [HL1568]

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Rabbit Recombinant Monoclonal WNT6 antibody. Suitable for WB, ICC/IF and reacts with Transfected cell lysate, Transfected cell line samples. Immunogen corresponding to Synthetic Peptide within Human WNT6 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Protein Wnt-6, WNT6

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-Wnt6 antibody [HL1568] (AB317091)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Wnt6 antibody [HL1568] (AB317091)

ab317091 detects Wnt6 protein by immunofluorescent analysis.
Sample : Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green : Wnt6 stained by ab317091 diluted at 1 : 500.
Blue : Fluoroshield with DAPI.

Western blot - Anti-Wnt6 antibody [HL1568] (AB317091)
  • WB

Supplier Data

Western blot - Anti-Wnt6 antibody [HL1568] (AB317091)

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with ab317091 diluted at 1 : 5000. An HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Wnt6 antibody [HL1568] (ab317091) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts

Lane 2:

Wnt 1 transfected (+) 293T whole cell extracts

Lane 3:

Wnt 2 transfected (+) 293T whole cell extracts

Lane 4:

Wnt 2b transfected (+) 293T whole cell extracts

Lane 5:

Wnt 3 transfected (+) 293T whole cell extracts

Lane 6:

Wnt 3a transfected (+) 293T whole cell extracts

Lane 7:

Wnt 4 transfected (+) 293T whole cell extracts

Lane 8:

Wnt 5a transfected (+) 293T whole cell extracts

Lane 9:

Wnt 6 transfected (+) 293T whole cell extracts

Lane 10:

Wnt 7a transfected (+) 293T whole cell extracts

Lane 11:

Wnt 7b transfected (+) 293T whole cell extracts

Lane 12:

Wnt 8a transfected (+) 293T whole cell extracts

Lane 13:

Wnt 8b transfected (+) 293T whole cell extracts

Lane 14:

Wnt 9a transfected (+) 293T whole cell extracts

Lane 15:

Wnt 9b transfected (+) 293T whole cell extracts

Lane 16:

Wnt 10a transfected (+) 293T whole cell extracts

Lane 17:

Wnt 10b transfected (+) 293T whole cell extracts

Lane 18:

Wnt 16 transfected (+) 293T whole cell extracts

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1568

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human WNT6 conjugated to Keyhole Limpet Haemocyanin.

Q9Y6F9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt6 also known simply as Wnt-6 is a member of the Wnt protein family characterized by its role in signaling pathways that regulate cell fate and patterning. It weighs approximately 50 kDa and is often found expressed in various tissues including the epidermis central nervous system and developing limbs. Wnt6 interacts with frizzled receptors on the cell surface to transmit signals that influence key cellular processes. Though the precise expression can vary it is generally consistent in tissues involved in development and repair.
Biological function summary

Wnt6 plays a significant role in embryonic development and tissue regeneration. By participating in the Wnt signaling pathways it helps in the control of cell division migration and differentiation. Wnt6 does not function alone but is part of a larger complex that includes other Wnt family members and receptors which collaborate to ensure proper signaling and cellular responses. Its activity is important during critical periods of development influencing the formation of various organs and tissues.

Pathways

Wnt6 is an important player in the canonical Wnt/β-catenin signaling pathway. This pathway is instrumental in regulating gene expression that determines cell behavior. Additionally Wnt6 is part of the planar cell polarity pathway interacting with other Wnt proteins like Wnt3a and signaling molecules such as Dishevelled and β-catenin. These pathways collectively regulate important cellular processes and maintain proper tissue architecture and functionality through coordinated signaling.

Wnt6 has been linked to various cancer types and developmental abnormalities. Its dysregulation can contribute to carcinogenesis particularly in breast cancer where altered Wnt6 expression impacts cellular proliferation and survival. Additionally its role in limb development suggests that mutations or misregulation might lead to limb deformities or skeletal disorders. Aberrant Wnt6 activity often correlates with changes in associated proteins such as β-catenin and frizzled receptors which together can offer potential targets for therapeutic intervention.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters. Together with CAV1 may promote chemoresistance of gastric cancer cells to DNA-damaging anthracycline drugs through the activation of the canonical Wnt receptor signaling pathway.
See full target information WNT6
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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