Anti-Wnt7a antibody

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody (AB100792)

ab100792 staining Wnt7a in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab100792 at 1µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue). Also suitable in cells fixed with 4% paraformaldehyde (10 min). Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

• WB

Unknown

Western blot - Anti-Wnt7a antibody (AB100792)

Exposure : 4 minutes

Blocking buffer : 2% BSA

Gel type : MOPS

Observed band size : 41 kDa

Additional bands : 50 kDa, 27kDa

All lanes:

Western blot - Anti-Wnt7a antibody (ab100792) at 1 µg/mL

Lane 1:

Human Kidney lysate at 10 µg

Lane 2:

Mouse Kidney lysate at 10 µg

Lane 3:

HepG2 whole cell lysate at 10 µg

Lane 4:

COLO 205 whole cell lysate at 10 µg

Lane 5:

Human Colon tumour tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

Predicted band size: 39 kDa

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• WB

Unknown

Western blot - Anti-Wnt7a antibody (AB100792)

All lanes:

Western blot - Anti-Wnt7a antibody (ab100792) at 1 µg/mL

All lanes:

Human kidney tissue lysate - total protein (<a href='/en-us/products/unavailable/human-kidney-tissue-lysate-total-protein-ab30203'>ab30203</a>) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 39 kDa

Observed band size: 15 kDa,28 kDa,41 kDa

true

Exposure time: 16min

• WB

CiteAb

Western blot - Anti-Wnt7a antibody (AB100792)

Wnt7a western blot using anti-Wnt7a antibody ab100792. Publication image and figure legend from Ma, A. Y., Xie, S. W., et al., 2017, Int J Mol Sci, PubMed 28640224.

ab100792 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab100792 please see the product overview.

The effects of NOMAC on the protein levels of SUFU and Wnt7a in cultured cells (RL95-2 and KLE), as assessed by Western blot. The relative amounts of SUFU and Wnt7a, collected from NOMAC-treated RL95-2 and KLE cells, were determined by Western blot for the indicated times. GAPDH was used as a loading control. Cells were cultured with DMSO (control) or graded concentrations of NOMAC (4, 20, and 100 μmol/L) for 6, 24, and 48 h. All of the results came from three independent experiments. The data were expressed as mean ± SD. (A) The protein expression of SUFU and Wnt7a in response to NOMAC treatment in RL95-2 cells; (B) NOMAC did not alter the expression levels of SUFU and Wnt7a as assessed by Western blot analysis in KLE Cells. * p < 0.05, ** p < 0.01 relative to the control group.

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