Rabbit Polyclonal WNT7A antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 21 publications.
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- The Journal of reproduction and development 68:21-292021Exosomes derived from placental trophoblast cells regulate endometrial epithelial receptivity in dairy cows during pregnancy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Su,Qianru Li,Qiaochu Zhang,Zhiming Li,Xinxin Yao,Yong Guo,Longfei Xiao,Xiangguo Wang,Hemin NiPubMed 34690214
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 8:e21011812021Reduced Oligodendrocyte Precursor Cell Impairs Astrocytic Development in Early Life Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuxin Wang,Yixun Su,Guangdan Yu,Xiaorui Wang,Xiaoying Chen,Bin Yu,Yijun Cheng,Rui Li,Juan C Sáez,Chenju Yi,Lan Xiao,Jianqin NiuPubMed 34155833
- International journal of molecular medicine 47:2021Wnt7a promotes the osteogenic differentiation of human mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLeiluo Yang,Qing Li,Junhong Zhang,Pengcheng Li,Pingjiang An,Chunqing Wang,Pingsheng Hu,Xue Zou,Xiaowei Dou,Lixin ZhuPubMed 33846764
- International journal of molecular medicine 45:578-5882020lncRNA BCAR4 sponges miR‑370‑3p to promote bladder cancer progression via Wnt signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRongkui Zhang,Jiping Wang,Er'na Jia,Jialiang Zhang,Nan Liu,Changliang ChiPubMed 31894304
- Hormones and behavior 114:1045452019Dickkopf-1 blocks 17β-estradiol-enhanced object memory consolidation in ovariectomized female mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLisa R Taxier et. al.PubMed 31228421
- Oncology letters 17:3219-32262019miR-127 suppresses gastric cancer cell migration and invasion via targeting Wnt7a.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLinlin Wang et. al.PubMed 30867752
- Neuroscience 394:164-1762018Wnt7a in Mouse Insular Cortex Contributes to Anxiety-like Behavior During Protracted Abstinence from Morphine.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Ma et. al.PubMed 30367944
- Cell reports 25:339-349.e92018A RECK-WNT7 Receptor-Ligand Interaction Enables Isoform-Specific Regulation of Wnt Bioavailability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMario Vallon,Kanako Yuki,Thi D Nguyen,Junlei Chang,Jenny Yuan,Dirk Siepe,Yi Miao,Markus Essler,Makoto Noda,K Christopher Garcia,Calvin J KuoPubMed 30304675
- Cancer cell 33:874-889.e72018A Glial Signature and Wnt7 Signaling Regulate Glioma-Vascular Interactions and Tumor Microenvironment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmelie Griveau et. al.PubMed 29681511
- The Journal of biological chemistry 293:6693-67062018Wnt7a activates canonical Wnt signaling, promotes bladder cancer cell invasion, and is suppressed by miR-370-3p.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojing Huang et. al.PubMed 29549123
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Tested | Expected |
| Chicken | Predicted | Predicted |
| Chimpanzee | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes Abcam recommends using 5% BSA as the blocking agent. |
Species Mouse | Dilution info 1 µg/mL | Notes Abcam recommends using 5% BSA as the blocking agent. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
ICC/IF
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species Mouse | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Chimpanzee, Gorilla, Orangutan | Dilution info - | Notes - |
Associated Products
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Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed:16826533). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:30026314). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes (By similarity). Required for normal neural stem cell proliferation in the hippocampus dentate gyrus (By similarity). Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation (By similarity). Promotes formation of synapses via its interaction with FZD5 (By similarity).
Alternative names
Protein Wnt-7a, WNT7A
Recommended products
Rabbit Polyclonal WNT7A antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Cited in 21 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt7a also known as Wingless-type MMTV integration site family member 7A is a protein involved in various cellular processes. It has a molecular mass of approximately 39 kDa and is expressed in several tissues including the brain lungs and kidneys. Mechanically Wnt7a acts as a signaling molecule that binds to frizzled receptors on cell surfaces initiating downstream signaling cascades that result in the transcriptional regulation of target genes.
- Biological function summary
This protein plays a critical role in the regulation of developmental processes including cell proliferation differentiation and migration. Wnt7a is part of the Wnt signaling pathway which is a complex network of proteins known for its involvement in embryonic development and regulation of cell fate decisions. The pathway contributes to the proper formation of tissues and organs and maintains cellular homeostasis.
- Pathways
Wnt7a is significantly involved in the canonical Wnt/β-catenin signaling pathway as well as the planar cell polarity pathway. In the canonical pathway Wnt7a influences the stabilization and translocation of β-catenin to the nucleus promoting transcription of target genes. It also interacts with other Wnt proteins like Wnt3a and different frizzled receptors playing roles in various cell signaling events and modulating cellular communication.
- Associated diseases and disorders
Wnt7a is linked to limb malformation disorders such as Fuhrmann syndrome and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome. These conditions arise from mutations that affect the function of Wnt7a disrupting normal limb development. Additionally aberrant Wnt7a signaling has implications in cancer particularly in the modulation of tumor microenvironment and metastasis where altered interactions with proteins like β-catenin can contribute to oncogenic processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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4 product images
Western blot - Anti-Wnt7a antibody (ab100792)
Exposure: 4 minutes
Blocking buffer: 2% BSA
Gel type: MOPS
Observed band size: 41 kDa
Additional bands: 50 kDa, 27kDa
All lanes: Western blot - Anti-Wnt7a antibody (ab100792) at 1 µg/mL
Lane 1: Human Kidney lysate at 10 µg
Lane 2: Mouse Kidney lysate at 10 µg
Lane 3: HepG2 whole cell lysate at 10 µg
Lane 4: COLO 205 whole cell lysate at 10 µg
Lane 5: Human Colon tumour tissue lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Predicted band size: 39 kDa
Western blot - Anti-Wnt7a antibody (ab100792)
All lanes: Western blot - Anti-Wnt7a antibody (ab100792) at 1 µg/mL
All lanes: Human kidney tissue lysate - total protein (ab30203) at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 15 kDa, 28 kDa, 41 kDa
Exposure time: 16min
Immunocytochemistry/ Immunofluorescence - Anti-Wnt7a antibody (ab100792)
ab100792 staining Wnt7a in HepG2 cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab100792 at 1µg/ml and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
Also suitable in cells fixed with 4% paraformaldehyde (10 min).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-Wnt7a antibody (ab100792)
Wnt7a western blot using anti-Wnt7a antibody ab100792. Publication image and figure legend from Ma, A. Y., Xie, S. W., et al., 2017, Int J Mol Sci, PubMed 28640224.
ab100792 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab100792 please see the product overview.
The effects of NOMAC on the protein levels of SUFU and Wnt7a in cultured cells (RL95-2 and KLE), as assessed by Western blot. The relative amounts of SUFU and Wnt7a, collected from NOMAC-treated RL95-2 and KLE cells, were determined by Western blot for the indicated times. GAPDH was used as a loading control. Cells were cultured with DMSO (control) or graded concentrations of NOMAC (4, 20, and 100 μmol/L) for 6, 24, and 48 h. All of the results came from three independent experiments. The data were expressed as mean ± SD. (A) The protein expression of SUFU and Wnt7a in response to NOMAC treatment in RL95-2 cells; (B) NOMAC did not alter the expression levels of SUFU and Wnt7a as assessed by Western blot analysis in KLE Cells. * p < 0.05, ** p < 0.01 relative to the control group.
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