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Rabbit Polyclonal WNT7A antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate, Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WNT7A aa 1 to C-terminus.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (AB183653), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (AB183653), expandable thumbnail
  • Western blot - Anti-Wnt7a antibody (AB183653), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (AB183653), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human WNT7A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00755

Consider this alternative

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Expected
Mouse
Expected
Tested
Transfected cell lysate
Tested
Not recommended

Tested
Tested

Species

Transfected cell lysate

Dilution info

-

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100 - 1/1000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Not recommended
Not recommended

Species

Transfected cell lysate

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed:16826533). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed:30026314). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes (By similarity). Required for normal neural stem cell proliferation in the hippocampus dentate gyrus (By similarity). Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation (By similarity). Promotes formation of synapses via its interaction with FZD5 (By similarity).

Alternative names

Recommended products

Rabbit Polyclonal WNT7A antibody. Suitable for WB, IHC-P and reacts with Transfected cell lysate, Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WNT7A aa 1 to C-terminus.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human WNT7A aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O00755
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Wnt7a also known as Wingless-type MMTV integration site family member 7A is a protein involved in various cellular processes. It has a molecular mass of approximately 39 kDa and is expressed in several tissues including the brain lungs and kidneys. Mechanically Wnt7a acts as a signaling molecule that binds to frizzled receptors on cell surfaces initiating downstream signaling cascades that result in the transcriptional regulation of target genes.

Biological function summary

This protein plays a critical role in the regulation of developmental processes including cell proliferation differentiation and migration. Wnt7a is part of the Wnt signaling pathway which is a complex network of proteins known for its involvement in embryonic development and regulation of cell fate decisions. The pathway contributes to the proper formation of tissues and organs and maintains cellular homeostasis.

Pathways

Wnt7a is significantly involved in the canonical Wnt/β-catenin signaling pathway as well as the planar cell polarity pathway. In the canonical pathway Wnt7a influences the stabilization and translocation of β-catenin to the nucleus promoting transcription of target genes. It also interacts with other Wnt proteins like Wnt3a and different frizzled receptors playing roles in various cell signaling events and modulating cellular communication.

Associated diseases and disorders

Wnt7a is linked to limb malformation disorders such as Fuhrmann syndrome and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome. These conditions arise from mutations that affect the function of Wnt7a disrupting normal limb development. Additionally aberrant Wnt7a signaling has implications in cancer particularly in the modulation of tumor microenvironment and metastasis where altered interactions with proteins like β-catenin can contribute to oncogenic processes.

Product promise

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4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (ab183653), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (ab183653)

    Paraffin-embedded mouse pancreas tissue stained for secreted WNT7A protein using ab183653 at 1/500 in immunohistochemical analysis.

    Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (ab183653), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (ab183653)

    Paraffin-embedded mouse stomach tissue stained for secreted WNT7A protein using ab183653 at 1/500 in immunohistochemical analysis.

    Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

  • Western blot - Anti-Wnt7a antibody (ab183653), expandable thumbnail

    Western blot - Anti-Wnt7a antibody (ab183653)

    10% SDS-PAGE

    All lanes: Western blot - Anti-Wnt7a antibody (ab183653) at 1/5000 dilution

    Lane 1: Non-transfected (–) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

    Lane 2: Transfected (+) HEK-293T whole cell extracts at 30 µg

    Predicted band size: 39 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (ab183653), expandable thumbnail
    Image from Kijong and Kyueng-Whan et al., J Breast Cancer., 20(4):361-367. Fig 1.; doi:10.4048/jbc.2017.20.4.361. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (ab183653)

    Kijong Yi, Kyueng-Whan Min, et al used ab183653 at 1/50 dilution in immunohistochemical analysis.

    Representative photographs of Wnt7a expression in the cytoplasm of neoplastic cells (from breast cancer patients diagnosed with invasive ductal carcinoma).

    (A) Negative, (B) Positive for Wnt7a. Original magnification ×200.

    This study, along with the waiver of informed consent, was approved by the Institutional Review Board at Hanyang University Hospital (HYUH 2016-05-009).

    Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

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Product protocols

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