Anti-Wnt7a antibody

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (AB183653)

Kijong Yi, Kyueng-Whan Min, et al used ab183653 at 1/50 dilution in immunohistochemical analysis.

Representative photographs of Wnt7a expression in the cytoplasm of neoplastic cells (from breast cancer patients diagnosed with invasive ductal carcinoma).

(A) Negative, (B) Positive for Wnt7a. Original magnification ×200.

This study, along with the waiver of informed consent, was approved by the Institutional Review Board at Hanyang University Hospital (HYUH 2016-05-009).

Reproduced under the Creative Commons license http : //creativecommons.org/licenses/by/4.0/.

Image from Kijong and Kyueng-Whan et al., J Breast Cancer., 20(4):361-367. Fig 1.; doi:10.4048/jbc.2017.20.4.361. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (AB183653)

Paraffin-embedded mouse stomach tissue stained for secreted WNT7A protein using ab183653 at 1/500 in immunohistochemical analysis.

Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (AB183653)

Paraffin-embedded mouse pancreas tissue stained for secreted WNT7A protein using ab183653 at 1/500 in immunohistochemical analysis.

Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• WB

Supplier Data

Western blot - Anti-Wnt7a antibody (AB183653)

10% SDS-PAGE

All lanes:

Western blot - Anti-Wnt7a antibody (ab183653) at 1/5000 dilution

Lane 1:

Non-transfected (–) HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extracts at 30 µg

Lane 2:

Transfected (+) HEK-293T whole cell extracts at 30 µg

Predicted band size: 39 kDa

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