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AB217844

Anti-Wnt7a antibody

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(3 Publications)

Rabbit Polyclonal WNT7A antibody. Suitable for IHC-P, WB and reacts with Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human WNT7A aa 200-300 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Protein Wnt-7a, WNT7A

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (AB217844)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt7a antibody (AB217844)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human kidney tissue, labeling Wnt7a using ab217844 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Western blot - Anti-Wnt7a antibody (AB217844)
  • WB

Supplier Data

Western blot - Anti-Wnt7a antibody (AB217844)

All lanes:

Western blot - Anti-Wnt7a antibody (ab217844) at 1/200 dilution

Lane 1:

rat brain lysates

Lane 2:

human colon carcinoma lysates

Secondary

All lanes:

goat anti-rabbit IgG antibody (H+L) HRP-conjugated at 1/3000 dilution

Predicted band size: 39 kDa

Observed band size: 41 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human WNT7A aa 200-300 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

O00755

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt7a also known as Wingless-type MMTV integration site family member 7A is a protein involved in various cellular processes. It has a molecular mass of approximately 39 kDa and is expressed in several tissues including the brain lungs and kidneys. Mechanically Wnt7a acts as a signaling molecule that binds to frizzled receptors on cell surfaces initiating downstream signaling cascades that result in the transcriptional regulation of target genes.
Biological function summary

This protein plays a critical role in the regulation of developmental processes including cell proliferation differentiation and migration. Wnt7a is part of the Wnt signaling pathway which is a complex network of proteins known for its involvement in embryonic development and regulation of cell fate decisions. The pathway contributes to the proper formation of tissues and organs and maintains cellular homeostasis.

Pathways

Wnt7a is significantly involved in the canonical Wnt/β-catenin signaling pathway as well as the planar cell polarity pathway. In the canonical pathway Wnt7a influences the stabilization and translocation of β-catenin to the nucleus promoting transcription of target genes. It also interacts with other Wnt proteins like Wnt3a and different frizzled receptors playing roles in various cell signaling events and modulating cellular communication.

Wnt7a is linked to limb malformation disorders such as Fuhrmann syndrome and Al-Awadi/Raas-Rothschild/Schinzel phocomelia syndrome. These conditions arise from mutations that affect the function of Wnt7a disrupting normal limb development. Additionally aberrant Wnt7a signaling has implications in cancer particularly in the modulation of tumor microenvironment and metastasis where altered interactions with proteins like β-catenin can contribute to oncogenic processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (By similarity). Plays an important role in embryonic development, including dorsal versus ventral patterning during limb development, skeleton development and urogenital tract development (PubMed : 16826533). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed : 30026314). Required for normal, sexually dimorphic development of the Mullerian ducts, and for normal fertility in both sexes (By similarity). Required for normal neural stem cell proliferation in the hippocampus dentate gyrus (By similarity). Required for normal progress through the cell cycle in neural progenitor cells, for self-renewal of neural stem cells, and for normal neuronal differentiation and maturation (By similarity). Promotes formation of synapses via its interaction with FZD5 (By similarity).
See full target information WNT7A
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e22837 PubMed36934389

2023

ZNF32 prevents the activation of cancer-associated fibroblasts through negative regulation of TGFB1 transcription in breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Li,Hang Yuan,Gang Zhao,Jie Zhang,Siqi Li,Di Gong,Tianyu Feng,Qiming Kou,Qijing Wang,Guanru Wang,Shan Li,Kai Li,Ping Lin

Scientific reports 8:15792 PubMed30361522

2018

High expression of WNT7A predicts poor prognosis and promote tumor metastasis in pancreatic ductal adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dong-Jin Wu,Yong-Sheng Jiang,Rui-Zhe He,Ling-Ye Tao,Min-Wei Yang,Xue-Liang Fu,Jiang-Yu Yang,Kun Zhu

International journal of clinical and experimental 11:4926-4933 PubMed31949568

2018

Inhibition of WNT7A-β-catenin signaling pathway sensitizes oral squamous cell carcinoma to cisplatin.

Applications

Unspecified application

Species

Unspecified reactive species

Jiangang Tian,Xiaoguang Cui,Yuandong Feng,Liufang Gu
View all publications
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