AB227607

Anti-Wnt7b antibody

Name: Anti-Wnt7b antibody (ab227607) | Abcam
Brand: Abcam Limited
SKU: AB227607
Price: 220 USD
Availability: InStock

Be the first to review this product! Submit a review

(5 Publications)

Rabbit Polyclonal WNT7B antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WNT7B.

View Alternative Names

Protein Wnt-7b, WNT7B

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-Wnt7b antibody (AB227607)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Wnt7b antibody (AB227607)

Immunofluorescence analysis of Mock and transfected 293T cells fixed in 4% paraformaldehyde at RT for 15 min labelling Wnt7b protein using ab227607 at a 1/500 dilution (Green). DAPI was used for nuclear staining (Blue).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human WNT7B. The exact immunogen used to generate this antibody is proprietary information.

P56706

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt7b also known as Wingless-type MMTV integration site family member 7B is a secreted glycoprotein involved in cell signaling. It has a molecular mass of around 39 kDa. Wnt7b is expressed in various tissues including the brain lung and reproductive organs. This protein serves an important role in mediating intercellular communication by binding to receptors on the cell surface which activates downstream signaling cascades.

Biological function summary

Wnt7b regulates many processes such as cell proliferation differentiation and migration. Part of the Wnt signaling complex it acts as a ligand that binds to Frizzled and LRP5/6 receptor complexes. This initiates a signaling cascade that influences gene expression thereby impacting cellular fate decisions. Wnt7b has significant roles during embryonic development and tissue homeostasis in adults.

Pathways

Several major pathways rely on Wnt7b for proper function including the canonical Wnt/β-catenin pathway and the non-canonical planar cell polarity pathway. Within these pathways Wnt7b interacts with several proteins such as β-catenin and Dishevelled to modulate cellular responses. This regulatory influence is key for maintaining normal physiological processes where the balance of pathway activation dictates the outcome of cellular events.

Many studies link Wnt7b to conditions like lung fibrosis and cancer. In lung fibrosis Wnt7b signaling contributes to fibroblast activation and extracellular matrix production processes commonly exacerbated in this disease. Meanwhile in some cancers aberrations in the Wnt/β-catenin pathway involving Wnt7b can lead to uncontrolled cell growth and tumor development with overexpression resulting in enhanced proliferation. Therefore Wnt7b and closely associated proteins like β-catenin become potential targets for therapeutic intervention in these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Ligand for members of the frizzled family of seven transmembrane receptors that functions in the canonical Wnt/beta-catenin signaling pathway (PubMed : 30026314). Required for normal fusion of the chorion and the allantois during placenta development (By similarity). Required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation (PubMed : 30026314).

See full target information WNT7B

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Investigative ophthalmology & visual science 66:19 PubMed40338179

2025

Regulatory Effects of the Wnt7b/β-Catenin/MMP-2 Signaling Pathway on Scleral Stiffness in Guinea Pigs With Form-Deprivation Myopia.

Applications

Unspecified application

Species

Unspecified reactive species

Binyu Wen,Hangyu Li,Hui Tao,Hong Ren,Bosheng Ma,Mengdi Shi,Shen Chen,Jiaqi Du,Ziyi Cai,Jing Zhang,Dongshi Guan,Zhihong Deng

Chinese medical journal 137:1973-1975 PubMed38763768

2024

An E2F7/TSC1-based model for predicting the efficacy of sirolimus after liver transplantation for hepatocellular carcinoma beyond the Milan criteria.

Applications

Unspecified application

Species

Unspecified reactive species

Lincheng Zhang,Wei Zhou,Shusen Zheng,Sunbin Ling,Xiao Xu

Stem cell research & therapy 14:214 PubMed37605285

2023

APOE deficiency impacts neural differentiation and cholesterol biosynthesis in human iPSC-derived cerebral organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Zhao,Tadafumi C Ikezu,Wenyan Lu,Jesse R Macyczko,Yonghe Li,Laura J Lewis-Tuffin,Yuka A Martens,Yingxue Ren,Yiyang Zhu,Yan W Asmann,Nilüfer Ertekin-Taner,Takahisa Kanekiyo,Guojun Bu

Developmental neuroscience 44:131-141 PubMed34929706

2021

Circ-Ctnnb1 Regulates Neuronal Injury in Spinal Cord Injury through the Wnt/β-Catenin Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jialong Qi,Tao Wang,Zhidong Zhang,Zongsheng Yin,Yiming Liu,Li Ma,Shaobao Pei,Zhou Dong,Guosong Han

Life sciences :117178 PubMed31862452

2019

WITHDRAWN: Overexpression of GLI2 induces odontogenic differentiation in human dental pulp stem cells through activation of the Wnt/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Haiying Zheng,Chao Tian,Xin Zeng,Tao Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt7b antibody

Immunocytochemistry/ Immunofluorescence - Anti-Wnt7b antibody (AB227607)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise