Rabbit Polyclonal WNT8A antibody. Suitable for IHC-P and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Human WNT8A aa 200-250 conjugated to Keyhole Limpet Haemocyanin.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human WNT8A aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H1J5
Reactivity data
Select an application| IHC-P | |
|---|---|
| Rat | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/100.00000 - 1/500.00000 | Notes When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors. Plays a role in embryonic patterning.
Alternative names
WNT8D, WNT8A, Protein Wnt-8a, Protein Wnt-8d
Recommended products
Rabbit Polyclonal WNT8A antibody. Suitable for IHC-P and reacts with Rat samples. Immunogen corresponding to Synthetic Peptide within Human WNT8A aa 200-250 conjugated to Keyhole Limpet Haemocyanin.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human WNT8A aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H1J5
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt8a also known as wingless-type MMTV integration site family member 8A is a member of the Wnt protein family. This protein has a mass of approximately 37 kDa. It is commonly expressed in embryonic tissues particularly in regions such as the somites and neural tube. Mechanically Wnt8a functions as a signaling molecule that is important for the transduction of signals through its interaction with Frizzled family receptors on the cell surface. This interaction initiates complex signaling cascades that lead to changes in gene expression.
- Biological function summary
The Wnt8a protein influences cell fate decisions and patterning during embryonic development. As an important player in the Wnt signaling pathway it participates in the regulation of cellular differentiation and proliferation. Wnt8a often operates in complexes with other Wnt proteins and co-receptors such as LRP5/6 which modulate the stability and localization of β-catenin. The stable accumulation of β-catenin in the cytoplasm leads to its translocation into the nucleus where it influences the transcription of target genes.
- Pathways
Wnt8a plays an integral role in the canonical Wnt/β-catenin signaling pathway. It is pivotal in the regulation of developmental processes and is associated with the establishment of the embryonic body axis. Through this pathway Wnt8a is related to proteins such as β-catenin and TCF/LEF transcription factors which are significant for gene regulatory networks during embryogenesis. Additionally Wnt8a interacts with the non-canonical Wnt/planar cell polarity (PCP) pathway which affects cellular movements and tissue architecture.
- Associated diseases and disorders
Aberrant Wnt8a signaling is implicated in congenital malformations and certain cancers. Dysregulation of this protein contributes to disorders such as neural tube defects which are linked to faulty cell patterning and migration. Moreover overactive Wnt8a signaling has connections to the development of colorectal cancer where it influences the abnormal proliferation of cells. During these pathological conditions Wnt8a interacts with other Wnt pathway components like Dvl and APC leading to disturbances in cellular homeostasis and uncontrolled cell growth.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt8a antibody (ab203589)
Immunohistochemical analysis of formalin-fixed, paraffin embedded rat brain tissue labeling Wnt8a with ab203589 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
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