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AB203589

Anti-Wnt8a antibody

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(1 Publication)

Rabbit Polyclonal WNT8A antibody. Suitable for IHC-P and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human WNT8A aa 200-250 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

WNT8D, WNT8A, Protein Wnt-8a, Protein Wnt-8d

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt8a antibody (AB203589)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt8a antibody (AB203589)

Immunohistochemical analysis of formalin-fixed, paraffin embedded rat brain tissue labeling Wnt8a with ab203589 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human WNT8A aa 200-250 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9H1J5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt8a also known as wingless-type MMTV integration site family member 8A is a member of the Wnt protein family. This protein has a mass of approximately 37 kDa. It is commonly expressed in embryonic tissues particularly in regions such as the somites and neural tube. Mechanically Wnt8a functions as a signaling molecule that is important for the transduction of signals through its interaction with Frizzled family receptors on the cell surface. This interaction initiates complex signaling cascades that lead to changes in gene expression.
Biological function summary

The Wnt8a protein influences cell fate decisions and patterning during embryonic development. As an important player in the Wnt signaling pathway it participates in the regulation of cellular differentiation and proliferation. Wnt8a often operates in complexes with other Wnt proteins and co-receptors such as LRP5/6 which modulate the stability and localization of β-catenin. The stable accumulation of β-catenin in the cytoplasm leads to its translocation into the nucleus where it influences the transcription of target genes.

Pathways

Wnt8a plays an integral role in the canonical Wnt/β-catenin signaling pathway. It is pivotal in the regulation of developmental processes and is associated with the establishment of the embryonic body axis. Through this pathway Wnt8a is related to proteins such as β-catenin and TCF/LEF transcription factors which are significant for gene regulatory networks during embryogenesis. Additionally Wnt8a interacts with the non-canonical Wnt/planar cell polarity (PCP) pathway which affects cellular movements and tissue architecture.

Aberrant Wnt8a signaling is implicated in congenital malformations and certain cancers. Dysregulation of this protein contributes to disorders such as neural tube defects which are linked to faulty cell patterning and migration. Moreover overactive Wnt8a signaling has connections to the development of colorectal cancer where it influences the abnormal proliferation of cells. During these pathological conditions Wnt8a interacts with other Wnt pathway components like Dvl and APC leading to disturbances in cellular homeostasis and uncontrolled cell growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Plays a role in embryonic patterning.
See full target information WNT8A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:109018 PubMed38357665

2024

and models define a molecular signature reference for human embryonic notochordal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Julie Warin,Nicolas Vedrenne,Vivian Tam,Mengxia Zhu,Danqing Yin,Xinyi Lin,Bluwen Guidoux-D'halluin,Antoine Humeau,Luce Roseiro,Lily Paillat,Claire Chédeville,Caroline Chariau,Frank Riemers,Markus Templin,Jérôme Guicheux,Marianna A Tryfonidou,Joshua W K Ho,Laurent David,Danny Chan,Anne Camus
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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