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AB227611

Anti-Wnt8a antibody

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(1 Publication)

Rabbit Polyclonal WNT8A antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human WNT8A.

View Alternative Names

WNT8D, WNT8A, Protein Wnt-8a, Protein Wnt-8d

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt8a antibody (AB227611)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt8a antibody (AB227611)

Paraffin-embedded human colon carcinoma tissue stained for Wnt8a with ab227611 at 1/250 dilution in immunohistochemical analysis.

Western blot - Anti-Wnt8a antibody (AB227611)
  • WB

Supplier Data

Western blot - Anti-Wnt8a antibody (AB227611)

10% SDS-PAGE

All lanes:

Western blot - Anti-Wnt8a antibody (ab227611) at 1/5000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

Human Wnt8a-transfected HEK-293T whole cell lysate at 30 µg

Predicted band size: 39 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human WNT8A. The exact immunogen used to generate this antibody is proprietary information.

Q9H1J5

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Primary Antibodies

AB109222

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Western blot - Anti-Wnt2/IRP antibody [EPR3101(2)] (AB109222)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt8a also known as wingless-type MMTV integration site family member 8A is a member of the Wnt protein family. This protein has a mass of approximately 37 kDa. It is commonly expressed in embryonic tissues particularly in regions such as the somites and neural tube. Mechanically Wnt8a functions as a signaling molecule that is important for the transduction of signals through its interaction with Frizzled family receptors on the cell surface. This interaction initiates complex signaling cascades that lead to changes in gene expression.
Biological function summary

The Wnt8a protein influences cell fate decisions and patterning during embryonic development. As an important player in the Wnt signaling pathway it participates in the regulation of cellular differentiation and proliferation. Wnt8a often operates in complexes with other Wnt proteins and co-receptors such as LRP5/6 which modulate the stability and localization of β-catenin. The stable accumulation of β-catenin in the cytoplasm leads to its translocation into the nucleus where it influences the transcription of target genes.

Pathways

Wnt8a plays an integral role in the canonical Wnt/β-catenin signaling pathway. It is pivotal in the regulation of developmental processes and is associated with the establishment of the embryonic body axis. Through this pathway Wnt8a is related to proteins such as β-catenin and TCF/LEF transcription factors which are significant for gene regulatory networks during embryogenesis. Additionally Wnt8a interacts with the non-canonical Wnt/planar cell polarity (PCP) pathway which affects cellular movements and tissue architecture.

Aberrant Wnt8a signaling is implicated in congenital malformations and certain cancers. Dysregulation of this protein contributes to disorders such as neural tube defects which are linked to faulty cell patterning and migration. Moreover overactive Wnt8a signaling has connections to the development of colorectal cancer where it influences the abnormal proliferation of cells. During these pathological conditions Wnt8a interacts with other Wnt pathway components like Dvl and APC leading to disturbances in cellular homeostasis and uncontrolled cell growth.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Plays a role in embryonic patterning.
See full target information WNT8A
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

IUBMB life 71:1916-1928 PubMed31317664

2019

MicroRNA-155 inhibition up-regulates LEPR to inhibit osteoclast activation and bone resorption via activation of AMPK in alendronate-treated osteoporotic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongxuan Mao,Yihai Zhu,Weimin Hao,Chao Chu,Hongmin Su
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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