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AB150606

Anti-Wnt8b antibody

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(1 Publication)

Rabbit Polyclonal WNT8B antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Protein Wnt-8b.

View Alternative Names

Protein Wnt-8b, WNT8B

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt8b antibody (AB150606)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt8b antibody (AB150606)

Immunohistochemical analysis of formalin-fixed paraffin embedded Human brain cortex (following heat-induced antigen retrieval) labelling Wnt8b using ab150606 at 20 μg/ml .

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human Protein Wnt-8b. The exact immunogen used to generate this antibody is proprietary information.

Q93098

Specificity

BLAST analysis of the peptide immunogen showed no homology with other Human proteins.

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Complementary Products

Primary Antibodies

AB150602

Anti-Wnt5b antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5b antibody (AB150602)

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Primary Antibodies

AB106928

Anti-Wnt9b antibody

Western blot - Anti-Wnt9b antibody (AB106928)

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Proteins & Peptides

AB159814

Recombinant Human Wnt9a protein

SDS-PAGE - Recombinant Human Wnt9a protein (AB159814)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10-20 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt8b known as Wingless-type MMTV integration site family member 8B is a secreted protein involved in the Wnt signaling pathway. Its molecular weight is approximately 37 kDa. It is expressed in several tissues including the developing central nervous system and somites during embryogenesis. Wnt8b belongs to the family of Wnt proteins which play a role in cell communication and gene expression regulation.
Biological function summary

Wnt8b has a significant role in the regulation of cell fate and patterning during development. It acts as a morphogen influencing the gradient-mediated processes that define tissue differentiation. As part of the canonical Wnt signaling pathway Wnt8b binds to the Frizzled receptors and co-receptors like LRP5/6 leading to stabilization of the beta-catenin complex in the cytoplasm. This beta-catenin complex translocates to the nucleus where it affects the transcription of target genes.

Pathways

Wnt8b is involved in two major signaling pathways: the canonical Wnt/beta-catenin pathway and the planar cell polarity pathway. In the Wnt/beta-catenin pathway Wnt8b interacts with proteins like Dishevelled affecting downstream effectors such as beta-catenin. In the planar cell polarity pathway Wnt8b influences cellular orientation and movement through interactions with proteins such as Rho and Rac contributing to tissue polarity and motility.

Wnt8b is associated with conditions like cancer and neurodevelopmental disorders. Aberrant Wnt8b expression can lead to tumor progression and metastasis with connections to oncogenes such as MYC and CTNNB1. In neurodevelopmental disorders disruptions in Wnt8b signaling can impact brain development and function relating to proteins that mediate synaptic communication and plasticity. Understanding these connections helps to elucidate the role of Wnt8b in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
See full target information Protein Wnt-8b
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 293:16528-16545 PubMed30166344

2018

The NFκB subunit RELA is a master transcriptional regulator of the committed epithelial-mesenchymal transition in airway epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Tian,Steven G Widen,Jun Yang,Thomas G Wood,Andrzej Kudlicki,Yingxin Zhao,Allan R Brasier
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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