Rabbit Polyclonal WNT8B antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Protein Wnt-8b.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human Protein Wnt-8b. The exact immunogen used to generate this antibody is proprietary information. Database link Q93098
Reactivity data
Select an application| IHC-P | |
|---|---|
| Human | Tested |
| Bat | Predicted |
| Common marmoset | Predicted |
| Dog | Predicted |
| Gorilla | Predicted |
| Horse | Predicted |
| Monkey | Predicted |
| Rabbit | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10.00000-20.00000 µg/mL | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rabbit, Dog, Monkey, Gorilla, Common marmoset, Bat, Horse | Dilution info - | Notes - |
Target data
Function
Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
Alternative names
Protein Wnt-8b, WNT8B
Recommended products
Rabbit Polyclonal WNT8B antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human Protein Wnt-8b.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.1% Sodium azide
Constituents: 99% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human Protein Wnt-8b. The exact immunogen used to generate this antibody is proprietary information. Database link Q93098
- Purification technique
- Affinity purification Immunogen
- Specificity
BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Wnt8b known as Wingless-type MMTV integration site family member 8B is a secreted protein involved in the Wnt signaling pathway. Its molecular weight is approximately 37 kDa. It is expressed in several tissues including the developing central nervous system and somites during embryogenesis. Wnt8b belongs to the family of Wnt proteins which play a role in cell communication and gene expression regulation.
- Biological function summary
Wnt8b has a significant role in the regulation of cell fate and patterning during development. It acts as a morphogen influencing the gradient-mediated processes that define tissue differentiation. As part of the canonical Wnt signaling pathway Wnt8b binds to the Frizzled receptors and co-receptors like LRP5/6 leading to stabilization of the beta-catenin complex in the cytoplasm. This beta-catenin complex translocates to the nucleus where it affects the transcription of target genes.
- Pathways
Wnt8b is involved in two major signaling pathways: the canonical Wnt/beta-catenin pathway and the planar cell polarity pathway. In the Wnt/beta-catenin pathway Wnt8b interacts with proteins like Dishevelled affecting downstream effectors such as beta-catenin. In the planar cell polarity pathway Wnt8b influences cellular orientation and movement through interactions with proteins such as Rho and Rac contributing to tissue polarity and motility.
- Associated diseases and disorders
Wnt8b is associated with conditions like cancer and neurodevelopmental disorders. Aberrant Wnt8b expression can lead to tumor progression and metastasis with connections to oncogenes such as MYC and CTNNB1. In neurodevelopmental disorders disruptions in Wnt8b signaling can impact brain development and function relating to proteins that mediate synaptic communication and plasticity. Understanding these connections helps to elucidate the role of Wnt8b in pathological states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt8b antibody (ab150606)
Immunohistochemical analysis of formalin-fixed paraffin embedded Human brain cortex (following heat-induced antigen retrieval) labelling Wnt8b using ab150606 at 20 μg/ml .
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