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Rabbit Monoclonal WNT8B antibody. Carrier free. Suitable for WB and reacts with Transfected cell line, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein Wnt-8b.

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Images

Western blot - Anti-Wnt8b antibody [HL1549] (AB308168), expandable thumbnail
  • Western blot - Anti-Wnt8b antibody [HL1549] (AB308168), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Constituents: 100% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human Protein Wnt-8b. The exact immunogen used to generate this antibody is proprietary information. Database link Q93098

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Expected
Transfected cell line
Tested

Tested
Tested

Species

Transfected cell line

Dilution info

1/1000 - 1/10000

Notes

-

Expected
Expected

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Target data

Function

Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.

Alternative names

Protein Wnt-8b, WNT8B

Recommended products

Rabbit Monoclonal WNT8B antibody. Carrier free. Suitable for WB and reacts with Transfected cell line, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein Wnt-8b.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human Protein Wnt-8b. The exact immunogen used to generate this antibody is proprietary information. Database link Q93098
Carrier free

Yes

Clone number

HL1549

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Wnt8b known as Wingless-type MMTV integration site family member 8B is a secreted protein involved in the Wnt signaling pathway. Its molecular weight is approximately 37 kDa. It is expressed in several tissues including the developing central nervous system and somites during embryogenesis. Wnt8b belongs to the family of Wnt proteins which play a role in cell communication and gene expression regulation.

Biological function summary

Wnt8b has a significant role in the regulation of cell fate and patterning during development. It acts as a morphogen influencing the gradient-mediated processes that define tissue differentiation. As part of the canonical Wnt signaling pathway Wnt8b binds to the Frizzled receptors and co-receptors like LRP5/6 leading to stabilization of the beta-catenin complex in the cytoplasm. This beta-catenin complex translocates to the nucleus where it affects the transcription of target genes.

Pathways

Wnt8b is involved in two major signaling pathways: the canonical Wnt/beta-catenin pathway and the planar cell polarity pathway. In the Wnt/beta-catenin pathway Wnt8b interacts with proteins like Dishevelled affecting downstream effectors such as beta-catenin. In the planar cell polarity pathway Wnt8b influences cellular orientation and movement through interactions with proteins such as Rho and Rac contributing to tissue polarity and motility.

Associated diseases and disorders

Wnt8b is associated with conditions like cancer and neurodevelopmental disorders. Aberrant Wnt8b expression can lead to tumor progression and metastasis with connections to oncogenes such as MYC and CTNNB1. In neurodevelopmental disorders disruptions in Wnt8b signaling can impact brain development and function relating to proteins that mediate synaptic communication and plasticity. Understanding these connections helps to elucidate the role of Wnt8b in pathological states.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-Wnt8b antibody [HL1549] (ab308168), expandable thumbnail

    Western blot - Anti-Wnt8b antibody [HL1549] (ab308168)

    Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8b antibody [HL1549] (ab308168) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-Wnt8b antibody [HL1549] (ab308168) at 1/5000 dilution

    Lane 1: HEK 293T cell lysate at 30 µg

    Lane 2: Wnt1 transfected HEK 293T cell lysate at 30 µg

    Lane 3: Wnt2 transfected HEK 293T cell lysate at 30 µg

    Lane 4: Wnt2b transfected HEK 293T cell lysate at 30 µg

    Lane 5: Wnt3 transfected HEK 293T cell lysate at 30 µg

    Lane 6: Wnt5a transfected HEK 293T cell lysate at 30 µg

    Lane 7: Wnt7a transfected HEK 293T cell lysate at 30 µg

    Lane 8: Wnt7b transfected HEK 293T cell lysate at 30 µg

    Lane 9: Wnt8a transfected HEK 293T cell lysate at 30 µg

    Lane 10: Wnt8b transfected HEK 293T cell lysate at 30 µg

    Lane 11: Wnt9a transfected HEK 293T cell lysate at 30 µg

    Lane 12: Wnt9b transfected HEK 293T cell lysate at 30 µg

    Lane 13: Wnt10a transfected HEK 293T cell lysate at 30 µg

    Lane 14: Wnt10b transfected HEK 293T cell lysate at 30 µg

    Lane 15: Wnt16 transfected HEK 293T cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

  • Western blot - Anti-Wnt8b antibody [HL1549] (ab308168), expandable thumbnail

    Western blot - Anti-Wnt8b antibody [HL1549] (ab308168)

    Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10%SDS-PAGE, and the membrane was blotted with Wnt8b antibody [HL1549] (ab308168) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    Lane 1: Non-transfected (–) 293T cell lysates at 30 µg

    Lane 2: Transfected (–) 293T cell lysates at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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