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AB308168

Anti-Wnt8b antibody [HL1549]

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Rabbit Recombinant Monoclonal WNT8B antibody. Carrier free. Suitable for WB and reacts with Transfected cell line, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Protein Wnt-8b.

View Alternative Names

Protein Wnt-8b, WNT8B

2 Images
Western blot - Anti-Wnt8b antibody [HL1549] (AB308168)
  • WB

Supplier Data

Western blot - Anti-Wnt8b antibody [HL1549] (AB308168)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Wnt8b antibody [HL1549] (ab308168) diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Wnt8b antibody [HL1549] (ab308168) at 1/5000 dilution

Lane 1:

HEK 293T cell lysate at 30 µg

Lane 2:

Wnt1 transfected HEK 293T cell lysate at 30 µg

Lane 3:

Wnt2 transfected HEK 293T cell lysate at 30 µg

Lane 4:

Wnt2b transfected HEK 293T cell lysate at 30 µg

Lane 5:

Wnt3 transfected HEK 293T cell lysate at 30 µg

Lane 6:

Wnt5a transfected HEK 293T cell lysate at 30 µg

Lane 7:

Wnt7a transfected HEK 293T cell lysate at 30 µg

Lane 8:

Wnt7b transfected HEK 293T cell lysate at 30 µg

Lane 9:

Wnt8a transfected HEK 293T cell lysate at 30 µg

Lane 10:

Wnt8b transfected HEK 293T cell lysate at 30 µg

Lane 11:

Wnt9a transfected HEK 293T cell lysate at 30 µg

Lane 12:

Wnt9b transfected HEK 293T cell lysate at 30 µg

Lane 13:

Wnt10a transfected HEK 293T cell lysate at 30 µg

Lane 14:

Wnt10b transfected HEK 293T cell lysate at 30 µg

Lane 15:

Wnt16 transfected HEK 293T cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Wnt8b antibody [HL1549] (AB308168)
  • WB

Supplier Data

Western blot - Anti-Wnt8b antibody [HL1549] (AB308168)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-Wnt8b antibody [HL1549] (ab308168) at 1/10000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

V5 tagged WNT8B transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Observed band size: 39 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1549

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human Protein Wnt-8b. The exact immunogen used to generate this antibody is proprietary information.

Q93098

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Transfected cell line": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt8b known as Wingless-type MMTV integration site family member 8B is a secreted protein involved in the Wnt signaling pathway. Its molecular weight is approximately 37 kDa. It is expressed in several tissues including the developing central nervous system and somites during embryogenesis. Wnt8b belongs to the family of Wnt proteins which play a role in cell communication and gene expression regulation.
Biological function summary

Wnt8b has a significant role in the regulation of cell fate and patterning during development. It acts as a morphogen influencing the gradient-mediated processes that define tissue differentiation. As part of the canonical Wnt signaling pathway Wnt8b binds to the Frizzled receptors and co-receptors like LRP5/6 leading to stabilization of the beta-catenin complex in the cytoplasm. This beta-catenin complex translocates to the nucleus where it affects the transcription of target genes.

Pathways

Wnt8b is involved in two major signaling pathways: the canonical Wnt/beta-catenin pathway and the planar cell polarity pathway. In the Wnt/beta-catenin pathway Wnt8b interacts with proteins like Dishevelled affecting downstream effectors such as beta-catenin. In the planar cell polarity pathway Wnt8b influences cellular orientation and movement through interactions with proteins such as Rho and Rac contributing to tissue polarity and motility.

Wnt8b is associated with conditions like cancer and neurodevelopmental disorders. Aberrant Wnt8b expression can lead to tumor progression and metastasis with connections to oncogenes such as MYC and CTNNB1. In neurodevelopmental disorders disruptions in Wnt8b signaling can impact brain development and function relating to proteins that mediate synaptic communication and plasticity. Understanding these connections helps to elucidate the role of Wnt8b in pathological states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
See full target information Protein Wnt-8b
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Product promise

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For full details, please see our Terms & Conditions

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