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AB317222

Anti-Wnt9a antibody [HL2422]

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Rabbit Monoclonal WNT9A antibody. Suitable for WB and reacts with Transfected cell lysate, Human samples. Immunogen corresponding to Synthetic Peptide within Human WNT9A.

View Alternative Names

WNT14, WNT9A, Protein Wnt-9a, Protein Wnt-14

3 Images
Western blot - Anti-Wnt9a antibody [HL2422] (AB317222)
  • WB

Supplier Data

Western blot - Anti-Wnt9a antibody [HL2422] (AB317222)

Various whole cell extracts (50 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317222 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

All lanes:

Western blot - Anti-Wnt9a antibody [HL2422] (ab317222) at 1/1000 dilution

Lane 1:

PANC-1 whole cell extracts at 50 µg

Lane 2:

Jurkat whole cell extracts at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

Western blot - Anti-Wnt9a antibody [HL2422] (AB317222)
  • WB

Supplier Data

Western blot - Anti-Wnt9a antibody [HL2422] (AB317222)

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE and the membrane was blotted with ab317222 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Wnt9a antibody [HL2422] (ab317222) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts

Lane 2:

Wnt1 transfected (+) 293T whole cell extracts

Lane 3:

Wnt2 transfected (+) 293T whole cell extracts

Lane 4:

Wnt2b transfected (+) 293T whole cell extracts

Lane 5:

Wnt3 transfected (+) 293T whole cell extracts

Lane 6:

Wnt3a transfected (+) 293T whole cell extracts

Lane 7:

Wnt4 transfected (+) 293T whole cell extracts

Lane 8:

Wnt5a transfected (+) 293T whole cell extracts

Lane 9:

Wnt6 transfected (+) 293T whole cell extracts

Lane 10:

Wnt7a transfected (+) 293T whole cell extracts

Lane 11:

Wnt7b transfected (+) 293T whole cell extracts

Lane 12:

Wnt8a transfected (+) 293T whole cell extracts

Lane 13:

Wnt8b transfected (+) 293T whole cell extracts

Lane 14:

Wnt9a transfected (+) 293T whole cell extracts

Lane 15:

Wnt9b transfected (+) 293T whole cell extracts

Lane 16:

Wnt10a transfected (+) 293T whole cell extracts

Lane 17:

Wnt10b transfected (+) 293T whole cell extracts

Lane 18:

Wnt16 transfected (+) 293T whole cell extracts

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Western blot - Anti-Wnt9a antibody [HL2422] (AB317222)
  • WB

Supplier Data

Western blot - Anti-Wnt9a antibody [HL2422] (AB317222)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE and the membrane was blotted with ab317222 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-Wnt9a antibody [HL2422] (ab317222) at 1/5000 dilution

Lane 1:

Non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

V5-tagged Wnt9a transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2422

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human WNT9A.

O14904

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt9a also known as Wingless-related integration site 9A is a secreted glycoprotein with a molecular mass of approximately 39 kDa. This protein forms part of the Wnt family which comprises signaling molecules involved in various developmental processes. Wnt9a is expressed in several tissues including the brain kidneys and skeletal tissues during embryonic development but it can also be found in adult tissues. Its expression pattern indicates a role in spatial and temporal regulation during cell growth and differentiation.
Biological function summary

Wnt9a functions in the regulation of cell proliferation migration and fate determination. It operates as part of larger protein complexes that include other Wnt family members and co-receptors such as Frizzled receptors. Wnt9a plays a significant role in osteoblast differentiation and skeletal development. It also contributes to the patterning of mesenchymal cells into chondrocytes during skeletal morphogenesis. These biological activities highlight its essential function in both developmental stages and tissue maintenance.

Pathways

Wnt9a acts through the canonical Wnt/β-catenin signaling pathway and is also involved in the non-canonical Wnt signaling pathway. Within these pathways it interacts with LRP5/6 receptors and related proteins like Dishevelled (Dvl) and Axin. The canonical pathway promotes gene transcription activation whereas the non-canonical pathway influences cell movements and organization. These interactions highlight Wnt9a’s role in the broader signaling cascades important for embryogenesis and homeostasis.

Wnt9a is associated with skeletal malformations such as Robinow syndrome and bone density disorders. Wnt9a mutations can disrupt normal skeletal development leading to abnormal growth and bone formation. In this context interactions with other proteins like BMP4 and Sox9 become disrupted contributing to the pathogenesis of these conditions. Studies continue to explore the therapeutic potential of targeting Wnt9a pathways for treating these skeletal disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ligand for members of the frizzled family of seven transmembrane receptors. Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal timing of IHH expression during embryonic bone development, normal chondrocyte maturation and for normal bone mineralization during embryonic bone development. Plays a redundant role in maintaining joint integrity.
See full target information WNT9A
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Product promise

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For full details, please see our Terms & Conditions

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