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Goat Polyclonal WNT9B antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human Protein Wnt-9b aa 150-200.

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Images

Western blot - Anti-Wnt9b antibody (AB106928), expandable thumbnail
  • Western blot - Anti-Wnt9b antibody (AB106928), expandable thumbnail

Key facts

Isotype

IgG

Host species

Goat

Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human Protein Wnt-9b aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link O14905

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested
Mouse
Tested
Rat
Predicted

Tested
Tested

Species

Mouse

Dilution info

0.30000-1.00000 µg/mL

Notes

1 hour primary incubation is recommended for this product.

Species

Human

Dilution info

0.30000-1.00000 µg/mL

Notes

1 hour primary incubation is recommended for this product.

Predicted
Predicted

Species

Rat

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal embryonic kidney development, and for normal development of the urogenital tract, including uterus and part of the oviduct and the upper vagina in females, and epididymis and vas deferens in males. Activates a signaling cascade in the metanephric mesenchyme that induces tubulogenesis. Acts upstream of WNT4 in the signaling pathways that mediate development of kidney tubules and the Muellerian ducts. Plays a role in cranofacial development and is required for normal fusion of the palate during embryonic development (By similarity).

Alternative names

Recommended products

Goat Polyclonal WNT9B antibody. Suitable for WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human Protein Wnt-9b aa 150-200.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human Protein Wnt-9b aa 150-200. The exact immunogen used to generate this antibody is proprietary information. Database link O14905
Purification technique

Affinity purification Immunogen

Concentration
Loading...
Purification notes

ab106928 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Wnt9b also known as Wingless-related integration site 9b is a protein important for cell signaling. It is part of the Wnt protein family with a mass of approximately 40 kDa. Scientists detect Wnt9b expression in several tissues including the kidney liver and certain cell types in the developing limb. The Wnt proteins play an important role in developmental processes through signaling pathways.

Biological function summary

Wnt9b impacts critical cellular events by mediating signaling processes. It interacts with other proteins forming complexes that influence gene expression and cell fate determination. Wnt9b significantly contributes to embryonic development particularly in organogenesis such as kidney and limb formation. Researchers have noted its role in maintaining the balance between proliferation and differentiation in those tissues.

Pathways

Current research has placed Wnt9b within the canonical Wnt/β-catenin signaling pathway and the non-canonical Wnt signaling pathway. These pathways involve important proteins like β-catenin in the canonical pathway which affects transcriptional regulation. Wnt9b also interacts with Frizzled receptors modulating downstream signaling effects. These pathways are essential in regulating cellular functions such as stem cell pluripotency and cellular differentiation.

Associated diseases and disorders

Scientists have linked Wnt9b to developmental disorders and cancer. In kidney development mutations or altered expression can lead to renal dysplasia or agenesis disorders connected with other pathways and factors such as BMP4. In cancer biology aberrant Wnt9b signaling correlates with tumorigenesis particularly in hepatocellular carcinoma where it may interact with proteins like AXIN2 leading to altered cell cycle progression and proliferation. Understanding Wnt9b's involvement in these conditions offers potential for therapeutic targets.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Western blot - Anti-Wnt9b antibody (ab106928), expandable thumbnail

    Western blot - Anti-Wnt9b antibody (ab106928)

    All lanes: Western blot - Anti-Wnt9b antibody (ab106928) at 1 µg/mL

    Lane 1: mouse kidney tissue lysate at 35 µg

    Lane 2: mouse brain tissue lysate at 35 µg

    Predicted band size: 39 kDa

    Observed band size: 38 kDa

  • Western blot - Anti-Wnt9b antibody (ab106928), expandable thumbnail

    Western blot - Anti-Wnt9b antibody (ab106928)

    Primary incubation was 1 hour.

    All lanes: Western blot - Anti-Wnt9b antibody (ab106928) at 0.3 µg/mL

    All lanes: Human Brain Cerebellum lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 39 kDa

    Observed band size: 38 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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