AB106928

Anti-Wnt9b antibody

Name: Anti-Wnt9b antibody (ab106928) | Abcam
Brand: Abcam Limited
SKU: AB106928

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(1 Publication)

Goat Polyclonal WNT9B antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human WNT9B aa 150-200.

View Alternative Names

WNT14B, WNT15, UNQ6973/PRO21956, WNT9B, Protein Wnt-9b, Protein Wnt-14b, Protein Wnt-15

2 Images

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Western blot - Anti-Wnt9b antibody (AB106928)

Primary incubation was 1 hour.

All lanes:

Western blot - Anti-Wnt9b antibody (ab106928) at 0.3 µg/mL

All lanes:

Human Brain Cerebellum lysate in RIPA buffer at 35 µg

Predicted band size: 39 kDa

Observed band size: 38 kDa

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Supplier Data

Western blot - Anti-Wnt9b antibody (AB106928)

All lanes:

Western blot - Anti-Wnt9b antibody (ab106928) at 1 µg/mL

Lane 1:

mouse kidney tissue lysate at 35 µg

Lane 2:

mouse brain tissue lysate at 35 µg

Predicted band size: 39 kDa

Observed band size: 38 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human WNT9B aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

O14905

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-1 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab106928 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt9b also known as Wingless-related integration site 9b is a protein important for cell signaling. It is part of the Wnt protein family with a mass of approximately 40 kDa. Scientists detect Wnt9b expression in several tissues including the kidney liver and certain cell types in the developing limb. The Wnt proteins play an important role in developmental processes through signaling pathways.

Biological function summary

Wnt9b impacts critical cellular events by mediating signaling processes. It interacts with other proteins forming complexes that influence gene expression and cell fate determination. Wnt9b significantly contributes to embryonic development particularly in organogenesis such as kidney and limb formation. Researchers have noted its role in maintaining the balance between proliferation and differentiation in those tissues.

Pathways

Current research has placed Wnt9b within the canonical Wnt/β-catenin signaling pathway and the non-canonical Wnt signaling pathway. These pathways involve important proteins like β-catenin in the canonical pathway which affects transcriptional regulation. Wnt9b also interacts with Frizzled receptors modulating downstream signaling effects. These pathways are essential in regulating cellular functions such as stem cell pluripotency and cellular differentiation.

Scientists have linked Wnt9b to developmental disorders and cancer. In kidney development mutations or altered expression can lead to renal dysplasia or agenesis disorders connected with other pathways and factors such as BMP4. In cancer biology aberrant Wnt9b signaling correlates with tumorigenesis particularly in hepatocellular carcinoma where it may interact with proteins like AXIN2 leading to altered cell cycle progression and proliferation. Understanding Wnt9b's involvement in these conditions offers potential for therapeutic targets.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal embryonic kidney development, and for normal development of the urogenital tract, including uterus and part of the oviduct and the upper vagina in females, and epididymis and vas deferens in males. Activates a signaling cascade in the metanephric mesenchyme that induces tubulogenesis. Acts upstream of WNT4 in the signaling pathways that mediate development of kidney tubules and the Muellerian ducts. Plays a role in cranofacial development and is required for normal fusion of the palate during embryonic development (By similarity).

See full target information WNT9B

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature medicine 22:163-74 PubMed26752519

2016

Mitochondrial iron chelation ameliorates cigarette smoke-induced bronchitis and emphysema in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Suzanne M Cloonan,Kimberly Glass,Maria E Laucho-Contreras,Abhiram R Bhashyam,Morgan Cervo,Maria A Pabón,Csaba Konrad,Francesca Polverino,Ilias I Siempos,Elizabeth Perez,Kenji Mizumura,Manik C Ghosh,Harikrishnan Parameswaran,Niamh C Williams,Kristen T Rooney,Zhi-Hua Chen,Monica P Goldklang,Guo-Cheng Yuan,Stephen C Moore,Dawn L Demeo,Tracey A Rouault,Jeanine M D'Armiento,Eric A Schon,Giovanni Manfredi,John Quackenbush,Ashfaq Mahmood,Edwin K Silverman,Caroline A Owen,Augustine M K Choi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt9b antibody

Western blot - Anti-Wnt9b antibody (AB106928)

Western blot - Anti-Wnt9b antibody (AB106928)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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