AB151220

Anti-Wnt9b antibody

Name: Anti-Wnt9b antibody (ab151220) | Abcam
Brand: Abcam Limited
SKU: AB151220

(1 Review)

(3 Publications)

Rabbit Polyclonal WNT9B antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human WNT9B.

View Alternative Names

WNT14B, WNT15, UNQ6973/PRO21956, WNT9B, Protein Wnt-9b, Protein Wnt-14b, Protein Wnt-15

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt9b antibody (AB151220)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt9b antibody (AB151220)

Immunohistochemistry of formalin-fixed, paraffin-embedded Human fetal kidney tissue staining Wnt9b using ab151220 at 10 ug/ml.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human WNT9B. The exact immunogen used to generate this antibody is proprietary information.

O14905

Specificity

BLAST analysis of the peptide immunogen showed no homology with other human proteins.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "10 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Bat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Elephant": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Horse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rabbit": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.1% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wnt9b also known as Wingless-related integration site 9b is a protein important for cell signaling. It is part of the Wnt protein family with a mass of approximately 40 kDa. Scientists detect Wnt9b expression in several tissues including the kidney liver and certain cell types in the developing limb. The Wnt proteins play an important role in developmental processes through signaling pathways.

Biological function summary

Wnt9b impacts critical cellular events by mediating signaling processes. It interacts with other proteins forming complexes that influence gene expression and cell fate determination. Wnt9b significantly contributes to embryonic development particularly in organogenesis such as kidney and limb formation. Researchers have noted its role in maintaining the balance between proliferation and differentiation in those tissues.

Pathways

Current research has placed Wnt9b within the canonical Wnt/β-catenin signaling pathway and the non-canonical Wnt signaling pathway. These pathways involve important proteins like β-catenin in the canonical pathway which affects transcriptional regulation. Wnt9b also interacts with Frizzled receptors modulating downstream signaling effects. These pathways are essential in regulating cellular functions such as stem cell pluripotency and cellular differentiation.

Scientists have linked Wnt9b to developmental disorders and cancer. In kidney development mutations or altered expression can lead to renal dysplasia or agenesis disorders connected with other pathways and factors such as BMP4. In cancer biology aberrant Wnt9b signaling correlates with tumorigenesis particularly in hepatocellular carcinoma where it may interact with proteins like AXIN2 leading to altered cell cycle progression and proliferation. Understanding Wnt9b's involvement in these conditions offers potential for therapeutic targets.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Ligand for members of the frizzled family of seven transmembrane receptors (Probable). Functions in the canonical Wnt/beta-catenin signaling pathway. Required for normal embryonic kidney development, and for normal development of the urogenital tract, including uterus and part of the oviduct and the upper vagina in females, and epididymis and vas deferens in males. Activates a signaling cascade in the metanephric mesenchyme that induces tubulogenesis. Acts upstream of WNT4 in the signaling pathways that mediate development of kidney tubules and the Muellerian ducts. Plays a role in cranofacial development and is required for normal fusion of the palate during embryonic development (By similarity).

See full target information WNT9B

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

British journal of cancer 130:1249-1260 PubMed38361045

2024

AHRR and SFRP2 in primary versus recurrent high-grade serous ovarian carcinoma and their prognostic implication.

Applications

Unspecified application

Species

Unspecified reactive species

Nanna Monjé,Mihnea P Dragomir,Bruno V Sinn,Inga Hoffmann,Anuar Makhmut,Tincy Simon,Catarina A Kunze,Jana Ihlow,Wolfgang D Schmitt,Jonathan Pohl,Iris Piwonski,Sofya Marchenko,Carlotta Keunecke,Teodor G Calina,Francesca Tiso,Hagen Kulbe,Caroline Kreuzinger,Dan Cacsire Castillo-Tong,Jalid Sehouli,Elena I Braicu,Carsten Denkert,Silvia Darb-Esfahani,Kirsten Kübler,David Capper,Fabian Coscia,Markus Morkel,David Horst,Christine Sers,Eliane T Taube

Dentistry journal 11: PubMed37366674

2023

Characterization of SHH, SOX3, WNT3A and WNT9B Proteins in Human Non-Syndromic Cleft Lip and Palate Tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Mārtiņš Vaivads,Ilze Akota,Māra Pilmane

Journal of the American Society of Nephrology : JA 28:2322-2336 PubMed28336721

2017

Tubule-Derived Wnts Are Required for Fibroblast Activation and Kidney Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Zhou,Haiyan Fu,Lu Zhang,Ke Zhang,Yali Min,Liangxiang Xiao,Lin Lin,Sheldon I Bastacky,Youhua Liu

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Wnt9b antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt9b antibody (AB151220)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise