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AB4731

Anti-WRNIP1/WHIP antibody

5

(1 Review)

|

(3 Publications)

Rabbit Polyclonal WRNIP1/WHIP antibody. Suitable for WB and reacts with Human samples. Cited in 3 publications.

View Alternative Names

WHIP, WRNIP1, ATPase WRNIP1, Werner helicase-interacting protein 1

1 Images
Western blot - Anti-WRNIP1/WHIP antibody (AB4731)
  • WB

Unknown

Western blot - Anti-WRNIP1/WHIP antibody (AB4731)

Western blot using ab4731 on HEK 293 lysate at 1/200.

~30μg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP protein. The main band could be blocked using the immunising peptide.

Western blot using ab4731 on HEK 293 lysate at 1/200.

~30μg of lysate was loaded per lane for 4-20% gradient SDS-PAGE. Comparison to a molecular weight marker (not shown) indicates a primary band of ~96.0 kDa is detected (indicated by an arrow). The identity of the minor band migrating at a slightly higher molecular weight is unknown, but may represent an alternate isoform of WRNIP1/WHIP or post translational modification of the WRNIP1/WHIP .

All lanes:

Western blot - Anti-WRNIP1/WHIP antibody (ab4731)

Predicted band size: 72 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Reactivity is expected against human, mouse, rat and monkey WHIP1 protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide immobilized to a solid phase.
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The WRNIP1/WHIP protein also known as Werner helicase interacting protein 1 (WRNIP1) plays a role in DNA repair processes. It has a molecular mass of approximately 72 kDa. WRNIP1 is expressed in multiple tissues with notable expression in the nucleus of proliferating cells. Mechanically the protein interacts with the Werner syndrome ATP-dependent helicase (WRN) and modulates its activity during the processing of stalled replication forks. Its interaction with DNA binding proteins is important to maintaining genomic stability.
Biological function summary

This protein aids cellular processes involved in DNA replication and repair. WRNIP1 forms part of a complex that includes proteins involved in the stabilization and restart of collapsed replication forks. The protein facilitates the stabilization and elongation of DNA strands playing a role in homologous recombination repair. The action of WRNIP1 extends to cell cycle regulation by ensuring repair precision before mitosis proceeds.

Pathways

WRNIP1 integrates into processes linked with the replication stress response and homologous recombination repair pathways. In these pathways it collaborates with proteins such as RAD51 an essential player in homologous recombination. Additionally WRNIP1 acts in cohesion with WRN influencing pathways that mitigate the risk of cancer by preventing excessive genomic alterations. These pathways are essential for the proper resolution of replication fork stalling situations.

WRNIP1 is closely associated with Werner syndrome and cancer predisposition. Its dysfunction can lead to defects in DNA repair mechanisms increasing susceptibility to such disorders. Along with WRN any alterations could potentially lead to faulty repair of DNA damage paving a way for oncogenesis. Researchers investigate WRNIP1 concerning its protective role against replication stress-induced genomic instability and its potential implications in drug resistance within cancer treatment strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a modulator of initiation or reinitiation events during DNA polymerase delta-mediated DNA synthesis. In the presence of ATP, stimulation of DNA polymerase delta-mediated DNA synthesis is decreased. Also plays a role in the innate immune defense against viruses. Stabilizes the RIGI dsRNA interaction and promotes RIGI 'Lys-63'-linked polyubiquitination. In turn, RIGI transmits the signal through mitochondrial MAVS.
See full target information WRNIP1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of the United States of America 119:e2117175119 PubMed36179048

2022

Novel biochemical, structural, and systems insights into inflammatory signaling revealed by contextual interaction proteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Rodolfo Ciuffa,Federico Uliana,Jonathan Mannion,Martin Mehnert,Tencho Tenev,Cathy Marulli,Ari Satanowski,Lena Maria Leone Keller,Pilar Natalia Rodilla Ramírez,Alessandro Ori,Matthias Gstaiger,Pascal Meier,Ruedi Aebersold

Oncogene 35:4009-19 PubMed26549024

2015

RAD18, WRNIP1 and ATMIN promote ATM signalling in response to replication stress.

Applications

Unspecified application

Species

Unspecified reactive species

N Kanu,T Zhang,R A Burrell,A Chakraborty,J Cronshaw,C DaCosta,E Grönroos,H N Pemberton,E Anderton,L Gonzalez,S Sabbioneda,H D Ulrich,C Swanton,A Behrens

Cancer science 98:1902-13 PubMed17888034

2007

Fibroblast growth factor receptor 1 oncogene partner as a novel prognostic biomarker and therapeutic target for lung cancer.

Applications

ICC/IF

Species

Human

Yuria Mano,Koji Takahashi,Nobuhisa Ishikawa,Atsushi Takano,Wataru Yasui,Kouki Inai,Hitoshi Nishimura,Eiju Tsuchiya,Yusuke Nakamura,Yataro Daigo
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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