Rabbit Recombinant Monoclonal WSX-1 antibody. Carrier free. Suitable for WB and reacts with Mouse samples. Cited in 1 publication.
Images
![Western blot - Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (AB273155), expandable thumbnail](https://content.abcam.com/products/images/wsx-1-antibody-epr20863-3-bsa-and-azide-free-ab273155--western-blot-img15727.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (AB273155)")
Publications
Filter by
- Blood 136:183-1982020Aging-induced IL27Ra signaling impairs hematopoietic stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHanqing He,Panglian Xu,Xiaofei Zhang,Min Liao,Qiongye Dong,Tingting Cong,Baixue Tang,Xiuxiu Yang,Maoqing Ye,Yingjun Chang,Weihua Liu,Xiaowo Wang,Zhenyu Ju,Jianwei WangPubMed 32305041
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | Flow Cyt | |
|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Receptor for IL27. Requires IL6ST/GP130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.
Alternative names
Tccr, Wsx1, Il27ra, Interleukin-27 receptor subunit alpha, IL-27 receptor subunit alpha, IL-27R subunit alpha, IL-27R-alpha, IL-27RA, Type I T-cell cytokine receptor, WSX-1, TCCR
Recommended products
Rabbit Recombinant Monoclonal WSX-1 antibody. Carrier free. Suitable for WB and reacts with Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20863-3
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab273155 is the carrier-free version of Anti-WSX-1 antibody [EPR20863-3] ab220359.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The WSX-1 protein also known as IL-27Rα or TCCR is a subunit of the interleukin-27 receptor. It weighs approximately 74 kDa and is mainly expressed on various immune cells including T cells B cells and monocytes. WSX-1 engages in the immune signaling by interacting specifically with its ligand IL-27 to drive immunological responses. This receptor belongs to the type I cytokine receptor family and is structurally characterized by certain conserved motifs important for its signaling functions.
- Biological function summary
WSX-1 plays a role in regulating immune cell activity and differentiation. It acts as a part of a receptor complex with gp130 to form the IL-27 receptor which transmits signals that modulate the activity of T helper cells. This signaling influences the production of pro-inflammatory and anti-inflammatory cytokines impacting immune system balance. The function of WSX-1 remains critical for controlling inflammatory responses and ensuring immune tolerance.
- Pathways
WSX-1 contributes to the JAK/STAT signaling pathway and influences the differentiation of Th1 cells. The connection to JAK/STAT signaling involves various proteins such as STAT1 and STAT3 which transduce IL-27-induced signals leading to gene expression changes in immune cells. Through its action in these pathways WSX-1 regulates immune responses and can influence inflammation dynamics in various biological contexts.
- Associated diseases and disorders
WSX-1 has associations with autoimmune diseases and infectious conditions. Alterations in its signaling pathways can lead to dysregulated immune responses contributing to diseases like multiple sclerosis and inflammatory bowel disease. The protein’s interaction with IL-27 is central in these conditions as it influences the pro-inflammatory and regulatory functions of the immune system. Furthermore studies suggest a link between WSX-1 activity and the modulation of responses against infections linking it to potential therapeutic targets for managing related disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155), expandable thumbnail](https://content.abcam.com/products/images/wsx-1-antibody-epr20863-3-bsa-and-azide-free-ab273155--western-blot-img15727.jpg "Western blot - Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)")
Western blot - Anti-WSX-1 antibody [EPR20863-3] - BSA and Azide free (ab273155)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Data from collaborator.
Exposure time: 1 minute.
WSX-1 = IL27Ra
These data were developed using the same antibody clone in a different buffer formulation containing PBS, glycyerol, BSA and sodium azide (Anti-WSX-1 antibody [EPR20863-3] ab220359).
All lanes: Western blot - Anti-WSX-1 antibody [EPR20863-3] (Anti-WSX-1 antibody [EPR20863-3] ab220359) at 1/1000 dilution
Lane 1: Wild-type mouse spleen (1) tissue lysate at 20 µg
Lane 2: Wild-type mouse spleen (2) tissue lysate at 20 µg
Lane 3: Wild-type mouse spleen (3) tissue lysate at 20 µg
Lane 4: IL27Ra knockout mouse spleen (1) tissue lysate at 20 µg
Lane 5: IL27Ra knockout mouse spleen (2) tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size: 69 kDa
Observed band size: 110-120 kDa
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