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AB155655

Anti-WTAP antibody

3

(1 Review)

|

(9 Publications)

Rabbit Polyclonal WTAP antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WTAP aa 1-250.

View Alternative Names

KIAA0105, WTAP, Pre-mRNA-splicing regulator WTAP, Female-lethal(2)D homolog, WT1-associated protein, Wilms tumor 1-associating protein, hFL(2)D

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody (AB155655)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody (AB155655)

Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling WTAP using ab155655 at a 1/500 dilution (green). The slide was costained with an alpha Tubulin antibody (red) at a 1/2000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody (AB155655)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody (AB155655)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human A549 Xenograft tissue, labeling WTAP using ab155655 at a 1/100 dilution.

Western blot - Anti-WTAP antibody (AB155655)
  • WB

Unknown

Western blot - Anti-WTAP antibody (AB155655)

10% SDS PAGE

All lanes:

Western blot - Anti-WTAP antibody (ab155655) at 1/1000 dilution

All lanes:

MOLT4 whole cell lysate at 30 µg

Predicted band size: 44 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human WTAP aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q15007

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p>Antigen retrieval is also possible using Tris-EDTA buffer (pH 8.0).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus tropicalis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WTAP also known as Wilms tumor 1-associated protein is a core component of the writer complex that catalyzes N6-methyladenosine (m6A) methylation on mRNA. With a molecular mass of approximately 39 kDa WTAP localizes almost ubiquitously in human tissues but shows higher expression in liver and kidney cells. This protein assists in the regulation of mRNA splicing and stability functioning as an adapter for RNA binding proteins.
Biological function summary

This protein plays a critical role in post-transcriptional gene regulation by being part of the m6A methyltransferase complex which includes other important proteins like METTL3 and METTL14. WTAP does not bind RNA directly but helps its partners achieve target specificity during gene expression processes. The presence of WTAP within the complex stabilizes and coordinates the activity of the entire methyltransferase assembly.

Pathways

The m6A methylation WTAP facilitates affects various pathways including mRNA splicing and degradation. This process intricately connects WTAP to the regulation of the JAK-STAT signaling pathway where its activity influences inflammatory responses and cell proliferation. Additionally WTAP has ties to the Wnt/β-catenin pathway collaborating with proteins like METTL3 to regulate gene expression involved in cell fate determination and embryogenesis.

Research shows WTAP has connections to various cancers such as hepatocellular carcinoma due to its role in mRNA processing and stability. This association often involves its interaction with other oncogenic proteins like METTL3 leading to aberrant gene expression patterns. WTAP also has relevance in acute myeloid leukemia where it influences mRNA maturation and impacts cell differentiation and proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed : 29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed : 24316715, PubMed : 24407421, PubMed : 24981863). Acts as a mRNA splicing regulator (PubMed : 12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed : 17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed : 17095724).
See full target information WTAP
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Journal of orthopaedic surgery and research 19:826 PubMed39639339

2024

WTAP mediates IL-1β-induced chondrocyte injury by enhancing CA12 mRNA stability depending on m6A modification.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Deng,Yizhou Xu,Zhengnan Li,Guangxuan Zeng

Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences 32:11-24 PubMed37926762

2023

Chidamide inhibits cell glycolysis in acute myeloid leukemia by decreasing N6-methyladenosine-related GNAS-AS1.

Applications

Unspecified application

Species

Unspecified reactive species

Changmei Hu,Xiao Fu,Shujun Li,Cong Chen,Xielan Zhao,Jie Peng

Microbiology spectrum :e0266622 PubMed36786625

2023

N-Methyladenosine RNA Modification in Host Cells Regulates Peste des Petits Ruminants Virus Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Owais Khan,Gunturu Narasimha Tanuj,Divyaprakash R Choravada,Kaushal Kishore Rajak,S Chandra Sekar,Madhu Cholenahalli Lingaraju,Sujoy K Dhara,Praveen K Gupta,Bishnu Prasad Mishra,Triveni Dutt,Ravi Kumar Gandham,Basavaraj Sajjanar

mBio 12:e0106721 PubMed34225491

2021

Methyltransferase-like 3 Modulates Severe Acute Respiratory Syndrome Coronavirus-2 RNA N6-Methyladenosine Modification and Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Xueyan Zhang,Haojie Hao,Li Ma,Yecheng Zhang,Xiao Hu,Zhen Chen,Di Liu,Jianhui Yuan,Zhangli Hu,Wuxiang Guan

Theranostics 10:8939-8956 PubMed32802173

2020

METTL14 aggravates endothelial inflammation and atherosclerosis by increasing FOXO1 N6-methyladeosine modifications.

Applications

Unspecified application

Species

Unspecified reactive species

Dongdong Jian,Ying Wang,Liguo Jian,Hao Tang,Lixin Rao,Ke Chen,Zhen Jia,Wanjun Zhang,Yiran Liu,Xu Chen,Xiwen Shen,Chuanyu Gao,Shuai Wang,Muwei Li

Cancer research 80:2790-2803 PubMed32169859

2020

A Novel Micropeptide Encoded by Y-Linked LINC00278 Links Cigarette Smoking and AR Signaling in Male Esophageal Squamous Cell Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Wu,Liyuan Zhang,Jieqiong Deng,Binbin Guo,Fang Li,Yirong Wang,Rui Wu,Shenghua Zhang,Jiachun Lu,Yifeng Zhou

Nucleic acids research 47:362-374 PubMed30364964

2018

N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication.

Applications

Unspecified application

Species

Unspecified reactive species

Haojie Hao,Sujuan Hao,Honghe Chen,Zhen Chen,Yanfang Zhang,Jun Wang,Hanzhong Wang,Bo Zhang,Jianming Qiu,Fei Deng,Wuxiang Guan

Cell communication and signaling : CCS 16:50 PubMed30143009

2018

Wilms' tumor 1-associating protein plays an aggressive role in diffuse large B-cell lymphoma and forms a complex with BCL6 via Hsp90.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Kuai,Xin Gong,Liya Ding,Fang Li,Lizhen Lei,Yuqi Gong,Qingmeng Liu,Huajiao Tan,Xinxia Zhang,Dongyu Liu,Guoping Ren,Hongyang Pan,Yaoyao Shi,Friederike Berberich-Siebelt,Zhengrong Mao,Ren Zhou

Oncology letters 13:2531-2538 PubMed28454430

2017

WT1-associated protein is a novel prognostic factor in pancreatic ductal adenocarcinoma.

Applications

IHC-P

Species

Human

Bing-Qi Li,Shuai Huang,Qian-Qian Shao,Jian Sun,Li Zhou,Lei You,Tai-Ping Zhang,Quan Liao,Jun-Chao Guo,Yu-Pei Zhao
View all publications
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Product promise

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