Rabbit Recombinant Monoclonal WTAP antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Images
![Western blot - Anti-WTAP antibody [EPR18744] - BSA and Azide free (AB232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--western-blot-img141073.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WTAP antibody [EPR18744] - BSA and Azide free (AB232392)")
![Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody [EPR18744] - BSA and Azide free (AB232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--immunocytochemistry-immunofluorescence-img57730.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody [EPR18744] - BSA and Azide free (AB232392)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (AB232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127284.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (AB232392)")
![Flow Cytometry (Intracellular) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (AB232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--flow-cytometry-intracellular-img51184.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (AB232392)")
![Immunoprecipitation - Anti-WTAP antibody [EPR18744] - BSA and Azide free (AB232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--immunoprecipitation-img53769.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-WTAP antibody [EPR18744] - BSA and Azide free (AB232392)")
Publications
Filter by
- Cancer chemotherapy and pharmacology 92:71-812023Tumor-associated macrophages promote cisplatin resistance in ovarian cancer cells by enhancing WTAP-mediated N6-methyladenosine RNA methylation via the CXCL16/CXCR6 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLan Hong,Xiuzhen Wang,Lang Zheng,Shengtan Wang,Genhai ZhuPubMed 37272931
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
1 product for Alternative Version
Target data
Function
Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).
Alternative names
KIAA0105, WTAP, Pre-mRNA-splicing regulator WTAP, Female-lethal(2)D homolog, WT1-associated protein, Wilms tumor 1-associating protein, hFL(2)D
Recommended products
Rabbit Recombinant Monoclonal WTAP antibody. Carrier free. Suitable for IP, WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18744
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232392 is the carrier-free version of Anti-WTAP antibody [EPR18744] ab195380.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WTAP also known as Wilms tumor 1-associated protein is a core component of the writer complex that catalyzes N6-methyladenosine (m6A) methylation on mRNA. With a molecular mass of approximately 39 kDa WTAP localizes almost ubiquitously in human tissues but shows higher expression in liver and kidney cells. This protein assists in the regulation of mRNA splicing and stability functioning as an adapter for RNA binding proteins.
- Biological function summary
This protein plays a critical role in post-transcriptional gene regulation by being part of the m6A methyltransferase complex which includes other important proteins like METTL3 and METTL14. WTAP does not bind RNA directly but helps its partners achieve target specificity during gene expression processes. The presence of WTAP within the complex stabilizes and coordinates the activity of the entire methyltransferase assembly.
- Pathways
The m6A methylation WTAP facilitates affects various pathways including mRNA splicing and degradation. This process intricately connects WTAP to the regulation of the JAK-STAT signaling pathway where its activity influences inflammatory responses and cell proliferation. Additionally WTAP has ties to the Wnt/β-catenin pathway collaborating with proteins like METTL3 to regulate gene expression involved in cell fate determination and embryogenesis.
- Associated diseases and disorders
Research shows WTAP has connections to various cancers such as hepatocellular carcinoma due to its role in mRNA processing and stability. This association often involves its interaction with other oncogenic proteins like METTL3 leading to aberrant gene expression patterns. WTAP also has relevance in acute myeloid leukemia where it influences mRNA maturation and impacts cell differentiation and proliferation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--western-blot-img141073.jpg "Western blot - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)")
Western blot - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)
Lane 1: Wild-type HAP1 whole cell lysate (20 μg)
Lane 2: WTAP knockout HAP1 whole cell lysate (20 μg)
Lane 3: MOLT-4 whole cell lysate (20 μg)
Lane 4: K562 whole cell lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - Anti-WTAP antibody [EPR18744] ab195380 observed at 50 kDa. Red - loading control, ab18058, observed at 130 kDa.
Anti-WTAP antibody [EPR18744] ab195380 was shown to specifically react with WTAP in wild-type HAP1 cells as signal was lost in WTAP knockout cells. Wild-type and WTAP knockout samples were subjected to SDS-PAGE. Anti-WTAP antibody [EPR18744] ab195380 and ab18058 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-WTAP antibody [EPR18744] ab195380).
All lanes: Western blot - Anti-WTAP antibody [EPR18744] (Anti-WTAP antibody [EPR18744] ab195380)
Predicted band size: 44 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--immunocytochemistry-immunofluorescence-img57730.jpg "Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)")
Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling WTAP with Anti-WTAP antibody [EPR18744] ab195380 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on Jurkat cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-WTAP antibody [EPR18744] ab195380 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-WTAP antibody [EPR18744] ab195380).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127284.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling WTAP with Anti-WTAP antibody [EPR18744] ab195380 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on human endometrium is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-WTAP antibody [EPR18744] ab195380).
![Flow Cytometry (Intracellular) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--flow-cytometry-intracellular-img51184.jpg "Flow Cytometry (Intracellular) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)")
Flow Cytometry (Intracellular) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cell line from peripheral blood) cells labeling WTAP with Anti-WTAP antibody [EPR18744] ab195380 at 1/600 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat Anti-Rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-WTAP antibody [EPR18744] ab195380).
![Immunoprecipitation - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--immunoprecipitation-img53769.jpg "Immunoprecipitation - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)")
Immunoprecipitation - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)
WTAP was immunoprecipitated from 0.35 mg of Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate with Anti-WTAP antibody [EPR18744] ab195380 at 1/40 dilution. Western blot was performed from the immunoprecipitate using Anti-WTAP antibody [EPR18744] ab195380 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Jurkat whole cell lysate 10μg (Input).
Lane 2: Anti-WTAP antibody [EPR18744] ab195380 IP in Jurkat whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-WTAP antibody [EPR18744] ab195380 in Jurkat whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-WTAP antibody [EPR18744] ab195380).
All lanes: Immunoprecipitation - Anti-WTAP antibody [EPR18744] (Anti-WTAP antibody [EPR18744] ab195380)
Predicted band size: 44 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--immunocytochemistry-immunofluorescence-img68784.jpg "Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)")
Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling WTAP with Anti-WTAP antibody [EPR18744] ab195380 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line. The nuclear counter stain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution (red).
The negative controls are as follows:
-ve control 1: Anti-WTAP antibody [EPR18744] ab195380 at 1/500 dilution, followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) secondary antibody at 1/1000 dilution.
-ve control 2: Anti-alpha Tubulin mouse MAb (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) at 1/1000 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-WTAP antibody [EPR18744] ab195380).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111952.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labeling WTAP with Anti-WTAP antibody [EPR18744] ab195380 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution. Nuclear staining on human liver cancer is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-WTAP antibody [EPR18744] ab195380).
![Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--immunocytochemistry-immunofluorescence-img68784.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392), expandable thumbnail](https://content.abcam.com/products/images/wtap-antibody-epr18744-bsa-and-azide-free-ab232392--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111952.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WTAP antibody [EPR18744] - BSA and Azide free (ab232392)")
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