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Knockout Tested Rabbit Recombinant Monoclonal WTAP phospho S306 antibody. Suitable for WB, IP, Dot and reacts with Human, Synthetic peptide - Human samples.

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Images

Dot Blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (AB316275), expandable thumbnail
  • Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (AB316275), expandable thumbnail
  • Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (AB316275), expandable thumbnail
  • Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (AB316275), expandable thumbnail
  • Immunoprecipitation - Anti-WTAP (phospho S306) antibody [EPR27038-188] (AB316275), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPDotFlow Cyt (Intra)ICC/IFIHC-P
Human
Tested
Tested
Expected
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Synthetic peptide - Human
Not recommended
Not recommended
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
1/1000
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Synthetic peptide - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Synthetic peptide - Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Synthetic peptide - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).

Alternative names

KIAA0105, WTAP, Pre-mRNA-splicing regulator WTAP, Female-lethal(2)D homolog, WT1-associated protein, Wilms tumor 1-associating protein, hFL(2)D

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal WTAP phospho S306 antibody. Suitable for WB, IP, Dot and reacts with Human, Synthetic peptide - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27038-188
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Dot Blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275), expandable thumbnail

    Dot Blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275)

    Dot blot analysis of WTAP (phospho S306) using ab316275 at 1:1000 (0.525 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:1000 dilution.
    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the

    detection of proteins in the mid-femtogram range.

    All lanes: Dot Blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275) at 1/1000 dilution

    Lane 1: WTAP (phospho S306) peptide a

    Lane 2: WTAP (phospho S306) peptide b

    Lane 3: WTAP (phospho S305) peptide

    Lane 4: WTAP non-phospho peptide

    Secondary

    All lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution

    Developed using the ECL technique.

    Exposure time: 180s

  • Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275), expandable thumbnail

    Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275)

    In Western blot, ab316275 was shown to bind specifically to WTAP. Target of interest was observed at 55 kDa in wild-type HAP1 cell lysates (lanes 1-2 ) with no signal observed at this size in WTAP knockout cell line lysates (lanes 3-4 ).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275) at 1/1000 dilution

    Lane 1: Untreated Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST

    Lane 2: Wild-type HAP1 treated with 200 ng/ml PMA for 30 minutes whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST

    Lane 3: Untreated WTAP knockout HAP1 whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST

    Lane 4: WTAP knockout HAP1 treated with 200 ng/ml PMA for 30 minutes whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST

    Lane 5: Untreated Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST

    Lane 6: Wild-type HAP1 treated with 200 ng/ml PMA for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST

    Lane 7: Untreated WTAP knockout HAP1 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST

    Lane 8: WTAP knockout HAP1 treated with 200 ng/ml PMA for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 55 kDa, 36 kDa

    Exposure time: 10s

  • Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275), expandable thumbnail

    Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275)

    In Western blot, Anti-WTAP antibody [EPR18744] - Loading Control (Anti-WTAP antibody [EPR18744] ab195380) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275) at 1/1000 dilution

    Lane 1: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST

    Lane 2: Jurkat whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 55 kDa

    Exposure time: 26s

  • Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275), expandable thumbnail

    Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275)

    In Western blot, Anti-WTAP antibody [EPR18744] - Loading Control (Anti-WTAP antibody [EPR18744] ab195380) staining at 1/1000 dilution.

    All lanes: Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275) at 1/1000 dilution

    Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST

    Lane 2: A375 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 55 kDa

    Exposure time: 26s

  • Immunoprecipitation - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275), expandable thumbnail

    Immunoprecipitation - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275)

    WTAP (phospho S306) was immunoprecipitated from 0.35 mg HAP1(human chronic myelogenous leukemia near-haploid cell) treated with 200 ng/ml PMA for 30 minutes whole cell lysate with ab316275 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab316275 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HAP1(human chronic myelogenous leukemia near-haploid cell) treated with 200 ng/ml PMA for 30 minutes whole cell lysate

    Lane 2: ab316275 IP in HAP1(human chronic myelogenous leukemia near-haploid cell) treated with 200 ng/ml PMA for 30 minutes whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab316275 in HAP1 treated with 200 ng/ml PMA for 30 minutes whole cell lysate

    All lanes: Immunoprecipitation - Anti-WTAP (phospho S306) antibody [EPR27038-188] (ab316275) at 1/30 dilution

    All lanes: HAP1(human chronic myelogenous leukemia near-haploid cell) treated with 200 ng/ml PMA for 30 minutes whole cell lysate with 5% NFDM/TBST

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366)

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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