Knockout Tested Rabbit Recombinant Monoclonal WTAP phospho S306 antibody. Carrier free. Suitable for WB, IP, Dot and reacts with Human, Synthetic peptide - Human samples.
Images
![Dot Blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (AB316276), expandable thumbnail](https://content.abcam.com/products/images/wtap-phospho-s306-antibody-epr27038-188-bsa-and-azide-free-ab316276--dot-blot-img426689.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (AB316276)")
![Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (AB316276), expandable thumbnail](https://content.abcam.com/products/images/wtap-phospho-s306-antibody-epr27038-188-bsa-and-azide-free-ab316276--western-blot-img426596.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (AB316276)")
![Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (AB316276), expandable thumbnail](https://content.abcam.com/products/images/wtap-phospho-s306-antibody-epr27038-188-bsa-and-azide-free-ab316276--western-blot-img426598.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (AB316276)")
![Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (AB316276), expandable thumbnail](https://content.abcam.com/products/images/wtap-phospho-s306-antibody-epr27038-188-bsa-and-azide-free-ab316276--western-blot-img426597.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (AB316276)")
![Immunoprecipitation - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (AB316276), expandable thumbnail](https://content.abcam.com/products/images/wtap-phospho-s306-antibody-epr27038-188-bsa-and-azide-free-ab316276--immunoprecipitation-img426670.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (AB316276)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | Dot | Flow Cyt (Intra) | ICC/IF | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human, Mouse, Rat | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).
Alternative names
KIAA0105, WTAP, Pre-mRNA-splicing regulator WTAP, Female-lethal(2)D homolog, WT1-associated protein, Wilms tumor 1-associating protein, hFL(2)D
Recommended products
Knockout Tested Rabbit Recombinant Monoclonal WTAP phospho S306 antibody. Carrier free. Suitable for WB, IP, Dot and reacts with Human, Synthetic peptide - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogens
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27038-188
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316276 is the carrirer-free version of Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WTAP also known as Wilms tumor 1-associated protein is a core component of the writer complex that catalyzes N6-methyladenosine (m6A) methylation on mRNA. With a molecular mass of approximately 39 kDa WTAP localizes almost ubiquitously in human tissues but shows higher expression in liver and kidney cells. This protein assists in the regulation of mRNA splicing and stability functioning as an adapter for RNA binding proteins.
- Biological function summary
This protein plays a critical role in post-transcriptional gene regulation by being part of the m6A methyltransferase complex which includes other important proteins like METTL3 and METTL14. WTAP does not bind RNA directly but helps its partners achieve target specificity during gene expression processes. The presence of WTAP within the complex stabilizes and coordinates the activity of the entire methyltransferase assembly.
- Pathways
The m6A methylation WTAP facilitates affects various pathways including mRNA splicing and degradation. This process intricately connects WTAP to the regulation of the JAK-STAT signaling pathway where its activity influences inflammatory responses and cell proliferation. Additionally WTAP has ties to the Wnt/β-catenin pathway collaborating with proteins like METTL3 to regulate gene expression involved in cell fate determination and embryogenesis.
- Associated diseases and disorders
Research shows WTAP has connections to various cancers such as hepatocellular carcinoma due to its role in mRNA processing and stability. This association often involves its interaction with other oncogenic proteins like METTL3 leading to aberrant gene expression patterns. WTAP also has relevance in acute myeloid leukemia where it influences mRNA maturation and impacts cell differentiation and proliferation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Dot Blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276), expandable thumbnail](https://content.abcam.com/products/images/wtap-phospho-s306-antibody-epr27038-188-bsa-and-azide-free-ab316276--dot-blot-img426689.jpg "Dot Blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276)")
Dot Blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276)
This data was developed using Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275, the same antibody clone in a different buffer formulation.
Dot blot analysis of WTAP (phospho S306) using Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275 at 1:1000 (0.525 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:1000 dilution.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for thedetection of proteins in the mid-femtogram range.
All lanes: Dot Blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275) at 1/1000 dilution
Lane 1: WTAP (phospho S306) peptide a
Lane 2: WTAP (phospho S306) peptide b
Lane 3: WTAP (phospho S305) peptide
Lane 4: WTAP non-phospho peptide
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/1000 dilution
Developed using the ECL technique.
Exposure time: 180s
![Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276), expandable thumbnail](https://content.abcam.com/products/images/wtap-phospho-s306-antibody-epr27038-188-bsa-and-azide-free-ab316276--western-blot-img426596.jpg "Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276)")
Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276)
This data was developed using Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275, the same antibody clone in a different buffer formulation.
In Western blot, Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275 was shown to bind specifically to WTAP. Target of interest was observed at 55 kDa in wild-type HAP1 cell lysates (lanes 1-2 ) with no signal observed at this size in WTAP knockout cell line lysates (lanes 3-4 ).
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.
All lanes: Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275) at 1/1000 dilution
Lane 1: Untreated Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 2: Wild-type HAP1 treated with 200 ng/ml PMA for 30 minutes whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 3: Untreated WTAP knockout HAP1 whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 4: WTAP knockout HAP1 treated with 200 ng/ml PMA for 30 minutes whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 5: Untreated Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
Lane 6: Wild-type HAP1 treated with 200 ng/ml PMA for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
Lane 7: Untreated WTAP knockout HAP1 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
Lane 8: WTAP knockout HAP1 treated with 200 ng/ml PMA for 30 minutes whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 55 kDa, 36 kDa
Exposure time: 10s
![Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276), expandable thumbnail](https://content.abcam.com/products/images/wtap-phospho-s306-antibody-epr27038-188-bsa-and-azide-free-ab316276--western-blot-img426598.jpg "Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276)")
Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276)
This data was developed using Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275, the same antibody clone in a different buffer formulation.
In Western blot, Anti-WTAP antibody [EPR18744] - Loading Control (Anti-WTAP antibody [EPR18744] ab195380) staining at 1/1000 dilution.
All lanes: Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275) at 1/1000 dilution
Lane 1: Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 2: Jurkat whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 55 kDa
Exposure time: 26s
![Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276), expandable thumbnail](https://content.abcam.com/products/images/wtap-phospho-s306-antibody-epr27038-188-bsa-and-azide-free-ab316276--western-blot-img426597.jpg "Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276)")
Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276)
This data was developed using Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275, the same antibody clone in a different buffer formulation.
In Western blot, Anti-WTAP antibody [EPR18744] - Loading Control (Anti-WTAP antibody [EPR18744] ab195380) staining at 1/1000 dilution.
All lanes: Western blot - Anti-WTAP (phospho S306) antibody [EPR27038-188] (Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275) at 1/1000 dilution
Lane 1: A375 (human malignant melanoma epithelial cell) whole cell lysate (untreated membrane) at 20 µg with 5% NFDM/TBST
Lane 2: A375 whole cell lysate (alkaline phosphatase treated membrane) at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 55 kDa
Exposure time: 26s
![Immunoprecipitation - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276), expandable thumbnail](https://content.abcam.com/products/images/wtap-phospho-s306-antibody-epr27038-188-bsa-and-azide-free-ab316276--immunoprecipitation-img426670.jpg "Immunoprecipitation - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276)")
Immunoprecipitation - Anti-WTAP (phospho S306) antibody [EPR27038-188] - BSA and Azide free (ab316276)
This data was developed using Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275, the same antibody clone in a different buffer formulation.
WTAP (phospho S306) was immunoprecipitated from 0.35 mg HAP1(human chronic myelogenous leukemia near-haploid cell) treated with 200 ng/ml PMA for 30 minutes whole cell lysate with Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: HAP1(human chronic myelogenous leukemia near-haploid cell) treated with 200 ng/ml PMA for 30 minutes whole cell lysate
Lane 2: Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275 IP in HAP1(human chronic myelogenous leukemia near-haploid cell) treated with 200 ng/ml PMA for 30 minutes whole cell lysate
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275 in HAP1 treated with 200 ng/ml PMA for 30 minutes whole cell lysateAll lanes: Immunoprecipitation - Anti-WTAP (phospho S306) antibody [EPR27038-188] (Anti-WTAP (phospho S306) antibody [EPR27038-188] ab316275) at 1/30 dilution
All lanes: HAP1(human chronic myelogenous leukemia near-haploid cell) treated with 200 ng/ml PMA for 30 minutes whole cell lysate with 5% NFDM/TBST
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366)
Exposure time: 180s
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