Knockout Tested Rabbit Recombinant Monoclonal WTAP phospho S341 antibody. Carrier free. Suitable for WB, IHC-P, IP, Dot and reacts with Human, Synthetic peptide samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
WB | IHC-P | IP | Dot | ICC/IF | Flow Cyt (Intra) | |
---|---|---|---|---|---|---|
Human | Tested | Tested | Tested | Expected | Not recommended | Not recommended |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
Synthetic peptide | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Synthetic peptide | Dilution info - | Notes - |
Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing (PubMed:29507755). Required for accumulation of METTL3 and METTL14 to nuclear speckle (PubMed:24316715, PubMed:24407421, PubMed:24981863). Acts as a mRNA splicing regulator (PubMed:12444081). Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532). Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes (PubMed:17095724).
KIAA0105, WTAP, Pre-mRNA-splicing regulator WTAP, Female-lethal(2)D homolog, WT1-associated protein, Wilms tumor 1-associating protein, hFL(2)D
Knockout Tested Rabbit Recombinant Monoclonal WTAP phospho S341 antibody. Carrier free. Suitable for WB, IHC-P, IP, Dot and reacts with Human, Synthetic peptide samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: 100% PBS
Liquid
Monoclonal
Yes
EPR27039-57
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
WTAP also known as Wilms tumor 1-associated protein is a core component of the writer complex that catalyzes N6-methyladenosine (m6A) methylation on mRNA. With a molecular mass of approximately 39 kDa WTAP localizes almost ubiquitously in human tissues but shows higher expression in liver and kidney cells. This protein assists in the regulation of mRNA splicing and stability functioning as an adapter for RNA binding proteins.
This protein plays a critical role in post-transcriptional gene regulation by being part of the m6A methyltransferase complex which includes other important proteins like METTL3 and METTL14. WTAP does not bind RNA directly but helps its partners achieve target specificity during gene expression processes. The presence of WTAP within the complex stabilizes and coordinates the activity of the entire methyltransferase assembly.
The m6A methylation WTAP facilitates affects various pathways including mRNA splicing and degradation. This process intricately connects WTAP to the regulation of the JAK-STAT signaling pathway where its activity influences inflammatory responses and cell proliferation. Additionally WTAP has ties to the Wnt/β-catenin pathway collaborating with proteins like METTL3 to regulate gene expression involved in cell fate determination and embryogenesis.
Research shows WTAP has connections to various cancers such as hepatocellular carcinoma due to its role in mRNA processing and stability. This association often involves its interaction with other oncogenic proteins like METTL3 leading to aberrant gene expression patterns. WTAP also has relevance in acute myeloid leukemia where it influences mRNA maturation and impacts cell differentiation and proliferation.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
This data was developed using Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-WTAP antibody (Anti-WTAP antibody [EPR18744] ab195380) as pan control staining at 1/1000 dilution.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-WTAP (phospho S341) antibody [EPR27039-57] (Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350) at 1/1000 dilution
Lane 1: Wild-type HAP1(human chronic myelogenous leukemia near-haploid cell line) whole cell lysate (untreated membrane) at 20 µg
Lane 2: Wild-type HAP1 whole cell lysate (Alkaline phosphatase treated membrane) at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Observed band size: 55 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-WTAP antibody (Anti-WTAP antibody [EPR18744] ab195380) as pan control staining at 1/1000 dilution.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
This data was developed using Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression of WTAP (phospho S341) is upregulated in response to PMA treatment (PMID:33217317).
In Western blot, anti-WTAP antibody (Anti-WTAP antibody [EPR18744] ab195380) as pan control staining at 1/1000 dilution.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
All lanes: Western blot - Anti-WTAP (phospho S341) antibody [EPR27039-57] (Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350) at 1/1000 dilution
Lane 1: Untreated Wild-type HAP1(human chronic myelogenous leukemia near-haploid cell line) whole cell lysate at 20 µg
Lane 2: HAP1 treated with /ml PMA for 30 minutes whole cell lysate at 20 µg
Lane 3: Untreated WTAP knockout HAP1 whole cell lysate at 20 µg
Lane 4: WTAP knockout HAP1 treated with /ml PMA for 30 minutes whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 55 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The expression of WTAP (phospho S341) is upregulated in response to PMA treatment (PMID:33217317).
In Western blot, anti-WTAP antibody (Anti-WTAP antibody [EPR18744] ab195380) as pan control staining at 1/1000 dilution.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Exposure time: 180 seconds
This data was developed using Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The samples were run on a Bis-Tris gel under reducing conditions.
Western blot: Anti-WTAP (phospho S341)antibody (Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody 6C5 (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.
The identity of the lower MW at approximately 35KD is unknown.
To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescentwestern blot (TBS-based) blocking solution before incubation with primaryantibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.
All lanes: Western blot - Anti-WTAP (phospho S341) antibody [EPR27039-57] (Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350) at 1/1000 dilution
All lanes: Jurkat (human T cell leukemia T lymphocyte) whole cell lysate at 20 µg
All lanes: Western blot - Goat Anti-Rat IgG H&L (Goat Anti-Rat IgG H&L ab97053) at 1/100000 dilution
Observed band size: 55 kDa
Blocking and diluting buffer and concentration: 5% NFDM/TBST
The samples were run on a Bis-Tris gel under reducing conditions.
Western blot: Anti-WTAP (phospho S341)antibody (Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody 6C5 (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.
The identity of the lower MW at approximately 35KD is unknown.
To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescentwestern blot (TBS-based) blocking solution before incubation with primaryantibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.
This data was developed using Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human endometrium tissue labeling WTAP (phospho S341) with Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350 at 1/500 (1.044 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human endometrium without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrumentIncubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human endometrial ca tissue labeling WTAP (phospho S341) with Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350 at 1/500 (1.044 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human endometrial carcinoma without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B).The section was incubated with Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
This data was developed using Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350, the same antibody clone in a different buffer formulation.
WTAP (phospho S341) was immunoprecipitated from 0.35 mg Parental HAP1 (wildtype control human chronic myelogenous leukemia near-haploid cell line) whole cell lysate with Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Parental HAP1 (wildtype control human chronic myelogenous leukemia near-haploid cell line) whole cell lysate 4 ug
Lane 2: abab309350 IP in Parental HAP1 (wildtype control human chronic myelogenous leukemia near-haploid cell line) whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350 in parental HAP1whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds
All lanes: Immunoprecipitation - Anti-WTAP (phospho S341) antibody [EPR27039-57] (Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350) at 1/30 dilution
All lanes: Parental HAP1 whole cell lysate at 4 µg
All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution
Observed band size: 55 kDa
Exposure time: 180s
WTAP (phospho S341) was immunoprecipitated from 0.35 mg Parental HAP1 (wildtype control human chronic myelogenous leukemia near-haploid cell line) whole cell lysate with Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Parental HAP1 (wildtype control human chronic myelogenous leukemia near-haploid cell line) whole cell lysate 4 ug
Lane 2: Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350 IP in Parental HAP1 (wildtype control human chronic myelogenous leukemia near-haploid cell line) whole cell lysate
Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350 in parental HAP1whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds
This data was developed using Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350, the same antibody clone in a different buffer formulation.
Dot blot analysis of WTAP (phospho S341) using Anti-WTAP (phospho S341) antibody [EPR27039-57] ab309350 at 1:1000 (0.522 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Exposure time: 180 seconds
Blocking and diluting buffer and concentration: 5% NFDM/TBST
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