JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB272629

Anti-WW domain binding protein 4 antibody

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Polyclonal WW domain binding protein 4 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human WBP4 aa 1-150.

View Alternative Names

FBP21, FNBP21, WBP4, WW domain-binding protein 4, WBP-4, Formin-binding protein 21, WW domain-containing-binding protein 4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-WW domain binding protein 4 antibody (AB272629)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-WW domain binding protein 4 antibody (AB272629)

PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) labeling WW domain binding protein 4 (green) using ab272629 at 2 ug/ml in ICC/IF analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WW domain binding protein 4 antibody (AB272629)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WW domain binding protein 4 antibody (AB272629)

Paraffin-embedded formalin-fixed human stomach tissue stained for WW domain binding protein 4 using ab272629 at 1/500 in immunohistochemical analysis.

Western blot - Anti-WW domain binding protein 4 antibody (AB272629)
  • WB

Unknown

Western blot - Anti-WW domain binding protein 4 antibody (AB272629)

All lanes:

Western blot - Anti-WW domain binding protein 4 antibody (ab272629) at 0.4 µg/mL

Lane 1:

NIH/3T3 (Mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II (Rat bladder carcinoma cell line) cell lysate

Predicted band size: 42 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human WBP4 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

O75554

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WW domain binding protein 4 (WBP4) also known by alternate names such as WWBP and Topoisomerase I-Interacting Protein (TOI1) is a protein with a mass of approximately 25 kDa. It participates in protein interactions through its WW domain facilitating the assembly of protein complexes involved in various cellular processes. WBP4 expression is relatively widespread observed in several tissues indicating its broad functional role across different cell types.
Biological function summary

WBP4 functions in pre-mRNA splicing events and participates as a component of the splicing machinery. Its interactions with other splicing factors modulate exon-intron recognition and influence the maturation of mRNA. Through these interactions WBP4 contributes to the regulation of gene expression at the transcriptional level influencing cellular functions by controlling the diversity of proteins generated from single genes.

Pathways

WBP4 has significant involvement in the spliceosome pathway where it plays a role in the regulation and assembly of splicing complexes. Additionally its interactions with proteins such as U2AF65 and SF3b1 highlight its importance within the splicing components that regulate RNA processing events. These relations assure proper RNA splicing and subsequent protein synthesis impacting numerous biological processes.

WBP4 associations include implications in cancer particularly in breast cancer where aberrant splicing events can contribute to disease progression. Its involvement with proteins like SF3b1 in the context of cancer highlights the importance of accurate splicing in preventing oncogenic transformations. Furthermore dysregulation of WBP4 has been observed in spinal muscular atrophy where splicing errors lead to motor neuron degeneration demonstrating the critical nature of splicing fidelity in maintaining health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in pre-mRNA splicing as a component of the spliceosome (PubMed : 19592703, PubMed : 28781166, PubMed : 9724750). May play a role in cross-intron bridging of U1 and U2 snRNPs in the mammalian A complex (PubMed : 9724750).
See full target information WBP4
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Histology and histopathology 40:2047-2058 PubMed40235277

2025

The mechanism of dexmedetomidine regulation of the HIF-1α/FUNDC1 axis in myocardial ischemia/reperfusion injury.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenfei Hu,Yidan Huang
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Proteins & Peptides

AB236179

Recombinant Human Wnt4 protein (Tagged)

SDS-PAGE - Recombinant Human Wnt4 protein (Tagged) (AB236179)

  • 0
  • 0
Proteins & Peptides

AB236190

Recombinant Human TRP14 protein (Tagged)

SDS-PAGE - Recombinant Human TRP14 protein (Tagged) (AB236190)

  • 0
  • 0
Proteins & Peptides

AB78710

Recombinant Human 14-3-3 eta/YWHAH protein

SDS-PAGE - Recombinant Human 14-3-3 eta/YWHAH protein (AB78710)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com