AB137726

Anti-WWOX antibody

Name: Anti-WWOX antibody (ab137726) | Abcam
Brand: Abcam Limited
SKU: AB137726
Price: 535 USD
Availability: InStock

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(6 Publications)

Rabbit Polyclonal WWOX antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Mouse samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WWOX aa 1-200.

View Alternative Names

FOR, SDR41C1, WOX1, WWOX, WW domain-containing oxidoreductase, Fragile site FRA16D oxidoreductase, Short chain dehydrogenase/reductase family 41C member 1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-WWOX antibody (AB137726)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-WWOX antibody (AB137726)

Immunofluorescent analysis of paraformaldehyde-fixed A549 cells labelling WWOX with ab137726 at 1/200 dilution. Lower panel shows the image merged with DNA probe.

Supplier Data

Immunoprecipitation - Anti-WWOX antibody (AB137726)

Samples were separated by 10% SDS-PAGE. The immunoprecipitated WWOX protein was detected by ab137726 diluted at 1/500 dilution.

All lanes:

Immunoprecipitation - Anti-WWOX antibody (ab137726) at 4 µg

Lane 1:

293T whole cell lysates with control IgG

Lane 2:

293T whole cell lysates with ab137726

Secondary

All lanes:

EasyBlot anti-Rabbit IgG

Predicted band size: 47 kDa

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Unknown

Western blot - Anti-WWOX antibody (AB137726)

12% SDS PAGE

All lanes:

Western blot - Anti-WWOX antibody (ab137726) at 1/1000 dilution

All lanes:

Raji whole cell lysate at 30 µg

Predicted band size: 47 kDa

false

Unknown

Western blot - Anti-WWOX antibody (AB137726)

12% SDS PAGE

All lanes:

Western blot - Anti-WWOX antibody (ab137726) at 1/10000 dilution

All lanes:

Mouse brain whole cell lysate at 50 µg

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human WWOX aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q9NZC7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/20000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein WWOX also known as WW domain-containing oxidoreductase or FOR has a molecular mass of approximately 46 kDa. Mechanically WWOX functions by interacting with several other proteins through its WW domains impacting various cellular processes. It exhibits a wide expression pattern found in numerous tissues including the brain lung and testis. Additionally WWOX localizes mainly in the cytoplasm but also migrates to the nucleus under certain conditions indicating its diverse roles in cellular regulation.

Biological function summary

The protein plays a significant role in cellular processes like apoptosis tumor suppression and steroidogenesis. WWOX participates in various cellular complexes including ones that regulate transcription and signal transduction. Its interaction with the tumor suppressor p53 and other signaling molecules supports its influence on cell cycle regulation and apoptotic pathways. This interaction aids the cell in sensing stress and damage modulating responses to these conditions.

Pathways

WWOX is intimately linked with the Wnt signaling and apoptotic pathways. In the Wnt pathway WWOX regulates cell proliferation and differentiation by interacting with proteins such as Dvl-2 and beta-catenin. In apoptotic pathways WWOX serves a regulatory role by modulating the activity of proteins like JNK and p73 which are important for inducing programmed cell death. These pathways highlight the importance of WWOX in maintaining cellular homeostasis and responding to abnormal cellular activities.

Alterations in WWOX expression have strong ties to cancer and neurodegenerative diseases. Loss or mutation of WWOX associates with breast prostate and lung cancers due to its role in tumor suppression. The protein also connects to neurodegenerative disorders such as Alzheimer's disease with misregulation affecting neuronal survival. In cancer interactions with proteins like ERBB4 further influence tumorigenic progression while in neurodegeneration WWOX interaction with Tau protein highlights its role in neuron integrity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Putative oxidoreductase. Acts as a tumor suppressor and plays a role in apoptosis. Required for normal bone development (By similarity). May function synergistically with p53/TP53 to control genotoxic stress-induced cell death. Plays a role in TGFB1 signaling and TGFB1-mediated cell death. May also play a role in tumor necrosis factor (TNF)-mediated cell death. Inhibits Wnt signaling, probably by sequestering DVL2 in the cytoplasm.

See full target information WWOX

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:13989 PubMed38886371

2024

In vitro evolution and whole genome analysis to study chemotherapy drug resistance in haploid human cells.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Carlos Jado,Michelle Dow,Krypton Carolino,Adam Klie,Gregory J Fonseca,Trey Ideker,Hannah Carter,Elizabeth A Winzeler

Cancer biology & therapy 21:927-936 PubMed32931356

2020

LncRNA WWOX-AS1 sponges miR-20b-5p in hepatocellular carcinoma and represses its progression by upregulating WWOX.

Applications

Unspecified application

Species

Unspecified reactive species

Dafeng Xu,Xiangmei Liu,Jincai Wu,Yu Wang,Kailun Zhou,Wenmei Chen,Jiacheng Chen,Cheng Chen,Liang Chen

Cell death & disease 8:e2955 PubMed28749468

2017

WWOX sensitises ovarian cancer cells to paclitaxel via modulation of the ER stress response.

Applications

Species

Unspecified reactive species

Szymon Janczar,Jaya Nautiyal,Yi Xiao,Edward Curry,Mingjun Sun,Elisa Zanini,Adam Jw Paige,Hani Gabra

International journal of molecular medicine 38:1850-1856 PubMed27840941

2016

Deregulated WWOX is involved in a negative feedback loop with microRNA-214-3p in osteosarcoma.

Applications

Species

Unspecified reactive species

Kaituo Gao,Jijuan Yin,Jian Dong

Cancer gene therapy 23:1-6 PubMed26516139

2015

Adenovirus-delivered wwox inhibited lung cancer growth in vivo in a mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Y Zhou,F Shou,H Zhang,Q You

Cell death and differentiation 21:1805-14 PubMed25012504

2014

Tumor suppressor WWOX regulates glucose metabolism via HIF1α modulation.

Applications

Unspecified application

Species

Unspecified reactive species

M Abu-Remaileh,R I Aqeilan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-WWOX antibody

Immunocytochemistry/ Immunofluorescence - Anti-WWOX antibody (AB137726)

Immunoprecipitation - Anti-WWOX antibody (AB137726)

Western blot - Anti-WWOX antibody (AB137726)

Western blot - Anti-WWOX antibody (AB137726)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise