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AB104440

Anti-WWP1 antibody

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(5 Publications)

Rabbit Polyclonal WWP1 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to WWP1.

View Alternative Names

NEDD4-like E3 ubiquitin-protein ligase WWP1, Atrophin-1-interacting protein 5, HECT-type E3 ubiquitin transferase WWP1, TGIF-interacting ubiquitin ligase 1, WW domain-containing protein 1, AIP5, Tiul1, WWP1

2 Images
Immunoprecipitation - Anti-WWP1 antibody (AB104440)
  • IP

Unknown

Immunoprecipitation - Anti-WWP1 antibody (AB104440)

Detection of Human WWP1 by Western blot of immunoprecipitates prepared from HeLa whole cell lysates (at 1 mg for IP, 20% of IP loaded) using ab104440 at 3 µg/mg lysate for immunoprecipitation and at 1 µg/ml for subsequent blotting.

Detection by chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-WWP1 antibody (ab104440)

Predicted band size: 105 kDa

false

Western blot - Anti-WWP1 antibody (AB104440)
  • WB

Unknown

Western blot - Anti-WWP1 antibody (AB104440)

All lanes:

Western blot - Anti-WWP1 antibody (ab104440) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 105 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human WWP1 aa 200 to C-terminus. Database link Q9H0M0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab104440 is affinity purified using an epitope specific to WWP1 immobilized on solid support
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WWP1 also known as NEDD4-like E3 ubiquitin-protein ligase operates as an E3 ubiquitin ligase. This protein adds ubiquitin to target substrates marking them for degradation by the proteasome. WWP1 has a molecular mass of approximately 99 kDa. It is expressed in various tissues including the brain heart and skeletal muscles contributing to various cellular processes through its enzymatic activity.
Biological function summary

This protein controls multiple cellular functions by targeting specific proteins for ubiquitination. WWP1 remains an important regulator in cell growth differentiation and immune response. It often acts within protein complexes and associates with multiple substrates and partners. These interactions allow it to control the stability and activity of proteins impacting cellular homeostasis.

Pathways

The ubiquitin-proteasome pathway features WWP1 prominently especially modulating cell cycle regulation and apoptosis. WWP1 also interacts within the TGF-beta signaling pathway impacting cell growth and differentiation. Through these pathways it connects with proteins such as SMAD2/3 and PTEN influencing critical cellular events and signaling processes.

Abnormal WWP1 activity links to cancer and neurodegenerative diseases. In cancer particularly breast and prostate cancer WWP1 can influence tumor growth due to its interactions with proteins like p53 and PTEN. For neurodegenerative diseases impaired WWP1 function affects protein aggregation and degradation contributing to diseases like Alzheimer's where it interacts with amyloid precursor protein (APP).
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF. Activates the Hippo signaling pathway in response to cell contact inhibition and recruitment to the Crumbs complex at the cell membrane (PubMed : 34404733). Monoubiquitinates AMOTL2 which facilitates its interaction with and activation of LATS2 (PubMed : 34404733). LATS2 then phosphorylates YAP1, excluding it from the nucleus and therefore ultimately represses YAP1-driven transcription of target genes (PubMed : 34404733).
See full target information WWP1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:29950 PubMed39622957

2024

WWP1 targeting PTEN for polyubiquitination to promote bone metastasis of luminal breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Jiang,Zhenxi Li,Wei Xu,Jianru Xiao

Nature communications 15:9950 PubMed39550386

2024

GPRC5A promotes lung colonization of esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Hongyu Zhou,Licheng Tan,Baifeng Zhang,Dora Lai Wan Kwong,Ching Ngar Wong,Yu Zhang,Beibei Ru,Yingchen Lyu,Kin To Hugo Siu,Jie Luo,Yuma Yang,Qin Liu,Yixin Chen,Weiguang Zhang,Chaohui He,Peng Jiang,Yanru Qin,Beilei Liu,Xin-Yuan Guan

Cell death discovery 8:107 PubMed35264565

2022

WWP1 upregulation predicts poor prognosis and promotes tumor progression by regulating ubiquitination of NDFIP1 in intrahepatic cholangiocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yongjian Li,Qian Cheng,Jie Gao,Zhuomiaoyu Chen,Jingheng Guo,Zuyin Li,Lingyu Tian,Chao Zhang,Yuzi Li,Jiaxi Zheng,Zhao Li,Jiye Zhu

Experimental & molecular medicine 53:973-985 PubMed34035464

2021

miR-19b enhances osteogenic differentiation of mesenchymal stem cells and promotes fracture healing through the WWP1/Smurf2-mediated KLF5/β-catenin signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Huang,Yongqiang Xu,Siyin Feng,Pan He,Bing Sheng,Jiangdong Ni

The Journal of biological chemistry 292:5253-5261 PubMed28193839

2017

Metformin Promotes AMP-activated Protein Kinase-independent Suppression of ΔNp63α Protein Expression and Inhibits Cancer Cell Viability.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Yi,Deshi Chen,Juan Ao,Shengnan Sun,Min Wu,Xiaorong Li,Johann Bergholz,Yujun Zhang,Zhi-Xiong Xiao
View all publications
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Product promise

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