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AB227213

Anti-WWP1 antibody

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(3 Publications)

Rabbit Polyclonal WWP1 antibody. Suitable for IHC-P and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human WWP1.

View Alternative Names

NEDD4-like E3 ubiquitin-protein ligase WWP1, Atrophin-1-interacting protein 5, HECT-type E3 ubiquitin transferase WWP1, TGIF-interacting ubiquitin ligase 1, WW domain-containing protein 1, AIP5, Tiul1, WWP1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWP1 antibody (AB227213)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWP1 antibody (AB227213)

Paraffin-embedded mouse stomach tissue stained for WWP1 using ab227213 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWP1 antibody (AB227213)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-WWP1 antibody (AB227213)

Paraffin-embedded mouse liver tissue stained for WWP1 using ab227213 at 1/500 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human WWP1. The exact immunogen used to generate this antibody is proprietary information.

Q9H0M0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

WWP1 also known as NEDD4-like E3 ubiquitin-protein ligase operates as an E3 ubiquitin ligase. This protein adds ubiquitin to target substrates marking them for degradation by the proteasome. WWP1 has a molecular mass of approximately 99 kDa. It is expressed in various tissues including the brain heart and skeletal muscles contributing to various cellular processes through its enzymatic activity.
Biological function summary

This protein controls multiple cellular functions by targeting specific proteins for ubiquitination. WWP1 remains an important regulator in cell growth differentiation and immune response. It often acts within protein complexes and associates with multiple substrates and partners. These interactions allow it to control the stability and activity of proteins impacting cellular homeostasis.

Pathways

The ubiquitin-proteasome pathway features WWP1 prominently especially modulating cell cycle regulation and apoptosis. WWP1 also interacts within the TGF-beta signaling pathway impacting cell growth and differentiation. Through these pathways it connects with proteins such as SMAD2/3 and PTEN influencing critical cellular events and signaling processes.

Abnormal WWP1 activity links to cancer and neurodegenerative diseases. In cancer particularly breast and prostate cancer WWP1 can influence tumor growth due to its interactions with proteins like p53 and PTEN. For neurodegenerative diseases impaired WWP1 function affects protein aggregation and degradation contributing to diseases like Alzheimer's where it interacts with amyloid precursor protein (APP).
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Ubiquitinates ERBB4 isoforms JM-A CYT-1 and JM-B CYT-1, KLF2, KLF5 and TP63 and promotes their proteasomal degradation. Ubiquitinates RNF11 without targeting it for degradation. Ubiquitinates and promotes degradation of TGFBR1; the ubiquitination is enhanced by SMAD7. Ubiquitinates SMAD6 and SMAD7. Ubiquitinates and promotes degradation of SMAD2 in response to TGF-beta signaling, which requires interaction with TGIF. Activates the Hippo signaling pathway in response to cell contact inhibition and recruitment to the Crumbs complex at the cell membrane (PubMed : 34404733). Monoubiquitinates AMOTL2 which facilitates its interaction with and activation of LATS2 (PubMed : 34404733). LATS2 then phosphorylates YAP1, excluding it from the nucleus and therefore ultimately represses YAP1-driven transcription of target genes (PubMed : 34404733).
See full target information WWP1
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:29950 PubMed39622957

2024

WWP1 targeting PTEN for polyubiquitination to promote bone metastasis of luminal breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Jiang,Zhenxi Li,Wei Xu,Jianru Xiao

American journal of physiology. Heart and circulatory physiology 319:H765-H774 PubMed32822210

2020

The ubiquitin ligase WWP1 contributes to shifts in matrix proteolytic profiles and a myocardial aging phenotype with diastolic heart.

Applications

Unspecified application

Species

Unspecified reactive species

Lydia E Matesic,Lisa A Freeburg,Laura B Snyder,Lauren-Ashley Duncan,Amber Moore,Paige E Perreault,Kia N Zellars,Edie C Goldsmith,Francis G Spinale

Neuroimmunomodulation 26:139-152 PubMed31340205

2019

CTBP1 Confers Protection for Hippocampal and Cortical Neurons in Rat Models of Alzheimer's Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Hu,Yafeng Li,Huifen Yu,Yanhui Hu
View all publications
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