AB103527

Anti-Wwp2 antibody

Name: Anti-Wwp2 antibody (ab103527) | Abcam
Brand: Abcam Limited
SKU: AB103527
Price: 430 USD
Availability: InStock

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(18 Publications)

Rabbit Polyclonal WWP2 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 18 publications. Immunogen corresponding to Synthetic Peptide within Human WWP2 aa 200-250.

View Alternative Names

NEDD4-like E3 ubiquitin-protein ligase WWP2, Atrophin-1-interacting protein 2, HECT-type E3 ubiquitin transferase WWP2, WW domain-containing protein 2, AIP2, WWP2

3 Images

Supplier Data

Immunoprecipitation - Anti-Wwp2 antibody (AB103527)

ab103527 at 1 used at 6 μg/reaction immunoprecipitating Wwp2 in HEK293T cell lysate.

Lane 1 : ab103527 + HEK293T cell lysate

Lane 2 : Rabbit anti-WWP2 Antibody instead of ab103527 in HEK293T cell lysate

Lane 3 : Control IgG instead of ab32562 in HEK293T cell lysate.

For blotting ab103527 was used at 1 μg/mL.

Detection : Chemiluminescence

Exposure time : 30 seconds

All lanes:

Immunoprecipitation - Anti-Wwp2 antibody (ab103527)

Predicted band size: 99 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-Wwp2 antibody (AB103527)

All lanes:

Western blot - Anti-Wwp2 antibody (ab103527) at 0.1 µg/mL

Lane 1:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 99 kDa

false

Exposure time: 30s

Supplier Data

Western blot - Anti-Wwp2 antibody (AB103527)

Cells were prepared using NETN lysis buffer.

Detection : Chemiluminescence

All lanes:

Western blot - Anti-Wwp2 antibody (ab103527) at 0.1 µg/mL

Lane 1:

293T cell lysate at 50 µg

Lane 2:

293T cell lysate at 15 µg

Lane 3:

293T cell lysate at 5 µg

Lane 4:

HeLa cell lysate at 50 µg

Lane 5:

Jurkat cell lysate at 50 µg

Predicted band size: 99 kDa

false

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human WWP2 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

O00308

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2.00000-5.00000 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000.00000 - 1/10000.00000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000.00000 - 1/10000.00000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Wwp2 also known as WWP2 or ubiquitin protein ligase E3 component N-terminal homolog 2 is a member of the NEDD4-like E3 ubiquitin ligases. It has a molecular weight of approximately 104 kDa. This protein facilitates the attachment of ubiquitin molecules to substrate proteins tagging them for proteasomal degradation or for regulation of their activity localization or interactions. Wwp2 is expressed in various tissues with higher expression in lung heart muscle and kidney indicating a broad functional range.

Biological function summary

Wwp2 plays a critical role in cellular processes like growth division and differentiation by regulating the stability of key signaling proteins. It functions as part of protein complexes that include WW domain-containing proteins which enables it to interact with multiple substrates. Through its ubiquitination activity Wwp2 influences pathways involved in the maintenance of cellular homeostasis and response to stimuli.

Pathways

Wwp2 has significant involvement in the TGF-beta signaling pathway and the Wnt signaling pathway. In the TGF-beta pathway Wwp2 mediates the degradation of SMAD2 and SMAD7 which are central to the regulation of this signaling cascade. Wwp2 also interacts with proteins like SMURF2 and RNF11 within these pathways promoting fine-tuned control of signal transduction ultimately influencing development and differentiation processes.

Wwp2 is linked to conditions such as cancer and cardiac hypertrophy. Dysregulation of Wwp2 can lead to aberrations in the TGF-beta pathway contributing to oncogenic processes and proliferation of tumor cells. Additionally Wwp2's role in the Wnt pathway associates it with cardiac hypertrophy where altered signaling may disturb cardiac muscle cell growth and function. Noteworthy Wwp2's interaction with proteins like SMAD7 in these contexts offers further insight into its pathological relevance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; in embryonic stem cells (ESCs) the ubiquitination is proposed to regulate POU5F1 protein level. Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.

See full target information WWP2

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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular diabetology 22:107 PubMed37149668

2023

Down-regulation of WWP2 aggravates Type 2 diabetes mellitus-induced vascular endothelial injury through modulating ubiquitination and degradation of DDX3X.

Applications

Unspecified application

Species

Unspecified reactive species

Shilong You,Jiaqi Xu,Zeyu Yin,Boquan Wu,Pengbo Wang,Mingjun Hao,Cheng Cheng,Mengke Liu,Yuanhui Zhao,Pengyu Jia,Hongkun Jiang,Da Li,Liu Cao,Xingang Zhang,Ying Zhang,Yingxian Sun,Naijin Zhang

Cell death discovery 8:421 PubMed36257929

2022

The role of E3 ubiquitin ligase WWP2 and the regulation of PARP1 by ubiquitinated degradation in acute lymphoblastic leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Xinxin Lu,Xinyue Huang,Haiqi Xu,Saien Lu,Shilong You,Jiaqi Xu,Qianru Zhan,Chao Dong,Ning Zhang,Ying Zhang,Liu Cao,Xingang Zhang,Naijin Zhang,Lijun Zhang

Acta biochimica et biophysica Sinica 54:1057-1067 PubMed35983977

2022

WWP2 ameliorates oxidative stress and inflammation in atherosclerotic mice through regulation of PDCD4/HO-1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xingye Wang,Lu Ma,Songlin Zhang,Qiang Song,Xumei He,Jun Wang

Cell biology international 46:1682-1692 PubMed35880837

2022

WWP2 overexpression inhibits the antitumor effects of doxorubicin in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yong Qin,Chao-Jun Wang,Hai-Lin Ye,Guan-Xiong Ye,Shi Wang,De-Biao Pan,Jun Wang,He-Juan Shen,Sheng-Qian Xu

The Journal of biological chemistry 298:101679 PubMed35124008

2022

FNDC5/irisin facilitates muscle-adipose-bone connectivity through ubiquitination-dependent activation of runt-related transcriptional factors RUNX1/2.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyu He,Yue Hua,Qian Li,Wei Zhu,Yu Pan,Yilin Yang,Xinyang Li,Mengxiao Wu,Jiyong Wang,Xiaoqing Gan

Experimental and therapeutic medicine 22:1254 PubMed34603522

2021

Etomidate inhibits cell proliferation and induces apoptosis in A549 non-small cell lung cancer cells via downregulating WWP2.

Applications

Unspecified application

Species

Unspecified reactive species

Deqiang Li,Junlong Zhang,Lijun Yin,Zhen Jin,Xuejun Chen,Xiangxue Meng

PLoS pathogens 17:e1009771 PubMed34314469

2021

CD97 stabilises the immunological synapse between dendritic cells and T cells and is targeted for degradation by the Salmonella effector SteD.

Applications

Unspecified application

Species

Unspecified reactive species

Ondrej Cerny,Camilla Godlee,Romina Tocci,Nancy E Cross,Haoran Shi,James C Williamson,Eric Alix,Paul J Lehner,David W Holden

FEBS letters 595:169-182 PubMed33107021

2020

ABRO1 stabilizes the deubiquitinase BRCC3 through inhibiting its degradation mediated by the E3 ubiquitin ligase WWP2.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Zhang,Shou-Song Tao,Ting Wang,Jie Zhang,Xian Liu,Ya-Ting Li,Hui Chen,Yi-Qun Zhan,Miao Yu,Chang-Hui Ge,Chang-Yan Li,Guang-Ming Ren,Xiao-Ming Yang,Rong-Hua Yin

Cell host & microbe 28:54-68.e7 PubMed32526160

2020

The Tumour Suppressor TMEM127 Is a Nedd4-Family E3 Ligase Adaptor Required by Salmonella SteD to Ubiquitinate and Degrade MHC Class II Molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Eric Alix,Camilla Godlee,Ondrej Cerny,Samkeliso Blundell,Romina Tocci,Sophie Matthews,Mei Liu,Jonathan N Pruneda,Kirby N Swatek,David Komander,Tabitha Sleap,David W Holden

Cell death and differentiation 27:3177-3195 PubMed32483383

2020

Ubiquitin ligase CHIP regulates OTUD3 stability and suppresses tumour metastasis in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Pengfei Zhang,Chaonan Li,Hongchang Li,Lin Yuan,Hongmiao Dai,Zhiqiang Peng,Zhikang Deng,Zhijie Chang,Chun-Ping Cui,Lingqiang Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

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