Rabbit Polyclonal XAB2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human XAB2 aa 800 to C-terminus.
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Publications
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- Nucleic acids research 49:9906-99252021XAB2 promotes Ku eviction from single-ended DNA double-strand breaks independently of the ATM kinase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAbhishek Bharadwaj Sharma,Hélène Erasimus,Lia Pinto,Marie-Christine Caron,Diyavarshini Gopaul,Thibaut Peterlini,Katrin Neumann,Petr V Nazarov,Sabrina Fritah,Barbara Klink,Christel C Herold-Mende,Simone P Niclou,Philippe Pasero,Patrick Calsou,Jean-Yves Masson,Sébastien Britton,Eric Van DyckPubMed 34500463
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human XAB2 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HCS7
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Chinese hamster | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Guinea pig | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rabbit | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Chinese hamster, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Rabbit, Guinea pig, Cow, Dog, Pig, Rhesus monkey, Chinese hamster, Orangutan | Dilution info - | Notes - |
Associated Products
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4 products for Alternative Product
Target data
Function
Involved in pre-mRNA splicing as component of the spliceosome (PubMed:11991638, PubMed:28076346, PubMed:28502770). Involved in transcription-coupled repair (TCR), transcription and pre-mRNA splicing (PubMed:10944529, PubMed:17981804).
Alternative names
HCNP, KIAA1177, SYF1, PP3898, XAB2, Pre-mRNA-splicing factor SYF1, Protein HCNP, XPA-binding protein 2
Recommended products
Rabbit Polyclonal XAB2 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human XAB2 aa 800 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human XAB2 aa 800 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HCS7
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The XAB2 protein also known as XPA-binding protein 2 is a multi-functional protein involved in several cellular processes. It has a molecular mass of approximately 98 kDa. XAB2 locates in both the nucleus and the cytoplasm of cells indicating its involvement in processes across different cellular compartments. The expression of XAB2 is observed in various tissues suggesting its role in several biological functions.
- Biological function summary
XAB2 participates in several critical processes including transcription splicing and DNA repair. It is a component of the transcription-coupled repair (TCR) machinery and plays a role in the pre-mRNA splicing process. XAB2 is part of a multiprotein complex that includes other important proteins such as the transcription factor IIH (TFIIH) and the excision repair cross-complementation group 2 (ERCC2). This complex ensures accuracy and efficiency in both gene expression and DNA repair processes.
- Pathways
XAB2 plays a significant role in the nucleotide excision repair (NER) and RNA splicing pathways. It interacts with proteins like ERCC3 and ERCC2 which are also important for NER. These pathways are essential for maintaining genomic stability by repairing DNA lesions and ensuring proper mRNA processing. XAB2's involvement in these pathways highlights its importance in safeguarding genomic integrity and accurate gene expression.
- Associated diseases and disorders
Alterations in XAB2 function relate to conditions such as cancer and Cockayne syndrome. Dysfunction in NER pathways where XAB2 plays a role can lead to genomic instability contributing to the development of various cancers. In Cockayne syndrome mutations in proteins related to the NER pathway like ERCC6 highlight the connection between DNA repair deficiencies and neurodegenerative disorders. Understanding XAB2's role offers insights into these complex diseases and could help in developing therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-XAB2 antibody (ab129487)
All lanes: Western blot - Anti-XAB2 antibody (ab129487) at 0.04 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HeLa whole cell lysate at 15 µg
Lane 3: 293T whole cell lysate at 50 µg
Lane 4: Jurkat whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 100 kDa
Exposure time: 30s
Immunoprecipitation - Anti-XAB2 antibody (ab129487)
Lane 1: ab129487 at 0.4 µg/ml staining XAB2 in HeLa whole cell lysate immunoprecipitated using ab129487 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: control IgG.
Detection: Chemiluminescence
Exposure time: 3 secondsAll lanes: Immunoprecipitation - Anti-XAB2 antibody (ab129487)
Predicted band size: 100 kDa
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