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AB250183

Anti-XAB2 antibody [EPR8971] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal XAB2 antibody. Carrier free. Suitable for IHC-P, ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

HCNP, KIAA1177, SYF1, PP3898, XAB2, Pre-mRNA-splicing factor SYF1, Protein HCNP, XPA-binding protein 2

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XAB2 antibody [EPR8971] - BSA and Azide free (AB250183)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XAB2 antibody [EPR8971] - BSA and Azide free (AB250183)

This data was developed using ab180181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human breast tissue labeling XAB2 with ab180181 at 1/50.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Western blot - Anti-XAB2 antibody [EPR8971] - BSA and Azide free (AB250183)
  • WB

Supplier Data

Western blot - Anti-XAB2 antibody [EPR8971] - BSA and Azide free (AB250183)

This data was developed using ab180181, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-XAB2 antibody [EPR8971] (<a href='/en-us/products/primary-antibodies/xab2-antibody-epr8971-ab180181'>ab180181</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 100 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-XAB2 antibody [EPR8971] - BSA and Azide free (AB250183)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-XAB2 antibody [EPR8971] - BSA and Azide free (AB250183)

This data was developed using ab180181, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells labeling XAB2 with ab180181 at 1/50.

Western blot - Anti-XAB2 antibody [EPR8971] - BSA and Azide free (AB250183)
  • WB

Supplier Data

Western blot - Anti-XAB2 antibody [EPR8971] - BSA and Azide free (AB250183)

This data was developed using ab180181, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human uterus tissue labeling XAB2 with ab180181 at 1/50.

All lanes:

Western blot - Anti-XAB2 antibody [EPR8971] (<a href='/en-us/products/primary-antibodies/xab2-antibody-epr8971-ab180181'>ab180181</a>)

Predicted band size: 100 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8971

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Antigen retrieval is recommended.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab250183 is the carrier-free version of ab180181.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The XAB2 protein also known as XPA-binding protein 2 is a multi-functional protein involved in several cellular processes. It has a molecular mass of approximately 98 kDa. XAB2 locates in both the nucleus and the cytoplasm of cells indicating its involvement in processes across different cellular compartments. The expression of XAB2 is observed in various tissues suggesting its role in several biological functions.
Biological function summary

XAB2 participates in several critical processes including transcription splicing and DNA repair. It is a component of the transcription-coupled repair (TCR) machinery and plays a role in the pre-mRNA splicing process. XAB2 is part of a multiprotein complex that includes other important proteins such as the transcription factor IIH (TFIIH) and the excision repair cross-complementation group 2 (ERCC2). This complex ensures accuracy and efficiency in both gene expression and DNA repair processes.

Pathways

XAB2 plays a significant role in the nucleotide excision repair (NER) and RNA splicing pathways. It interacts with proteins like ERCC3 and ERCC2 which are also important for NER. These pathways are essential for maintaining genomic stability by repairing DNA lesions and ensuring proper mRNA processing. XAB2's involvement in these pathways highlights its importance in safeguarding genomic integrity and accurate gene expression.

Alterations in XAB2 function relate to conditions such as cancer and Cockayne syndrome. Dysfunction in NER pathways where XAB2 plays a role can lead to genomic instability contributing to the development of various cancers. In Cockayne syndrome mutations in proteins related to the NER pathway like ERCC6 highlight the connection between DNA repair deficiencies and neurodegenerative disorders. Understanding XAB2's role offers insights into these complex diseases and could help in developing therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in pre-mRNA splicing as component of the spliceosome (PubMed : 11991638, PubMed : 28076346, PubMed : 28502770). Involved in transcription-coupled repair (TCR), transcription and pre-mRNA splicing (PubMed : 10944529, PubMed : 17981804).
See full target information XAB2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental and therapeutic medicine 23:157 PubMed35069838

2022

APPL1 ameliorates myocardial ischemia-reperfusion injury by regulating the AMPK signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yunguang Cen,Wei Liao,Taihao Wang,Daimin Zhang
View all publications
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Product promise

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