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Rabbit Recombinant Monoclonal XAF1 antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.

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Images

Western blot - Anti-XAF1 antibody [EPR26960-175] - BSA and Azide free (AB313880), expandable thumbnail
  • Immunoprecipitation - Anti-XAF1 antibody [EPR26960-175] - BSA and Azide free (AB313880), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PFlow Cyt (Intra)ICC/IF
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondria, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.

Alternative names

BIRC4BP, XIAPAF1, XAF1, XIAP-associated factor 1, BIRC4-binding protein

Recommended products

Rabbit Recombinant Monoclonal XAF1 antibody. Carrier free. Suitable for WB, IP and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26960-175
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The XAF1 protein also known as XIAP associated factor 1 plays a role in apoptosis regulation. It weighs approximately 34 kDa and exists in multiple isoforms. The protein is broadly expressed in various tissues including the liver pancreas and heart. XAF1 interacts directly with XIAP (X-linked inhibitor of apoptosis) counteracting its anti-apoptotic effects. XAF1's activity centers on promoting apoptosis by binding to XIAP and inhibiting its function.

Biological function summary

XAF1 functions in promoting cell death through apoptosis. It forms part of the apoptosome a large protein complex important for the intrinsic pathway of apoptosis. XAF1 exerts its influence by destabilizing XIAP's interaction with caspases releasing them to activate. These interactions trigger a cascade that leads to programmed cell death vital for maintaining cellular balance and response to stress.

Pathways

XAF1 plays significant role in the apoptosis and cell survival pathways. Within the intrinsic pathway XAF1 associates with Bax and caspase-3 directly impacting mitochondrial membrane permeability and caspase activation. These pathways ensure proper cell turnover and reaction to cellular damage often involving Bcl-2 family proteins and Smac/DIABLO protein for regulation as well.

Associated diseases and disorders

XAF1's role in cancer and neurological disorders is noteworthy. In several cancer types such as colon and gastric cancers decreased XAF1 expression associates with increased cancer cell survival due to impaired apoptosis. Meanwhile abnormal regulation of XAF1 links to neurodegenerative diseases involving proteins like XIAP and caspase-9 where inhibited apoptosis can lead to accumulation of damaged cells contributing to disease progression.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-XAF1 antibody [EPR26960-175] - BSA and Azide free (ab313880), expandable thumbnail

    Western blot - Anti-XAF1 antibody [EPR26960-175] - BSA and Azide free (ab313880)

    This data was developed using Anti-XAF1 antibody [EPR26960-175] ab313879, the same antibody clone in a different buffer formulation.
    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative control: K-562 (PMID:11175744).
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: Lanes 1-2: 59 seconds;
    Lanes 3-5: 6 seconds.

    All lanes: Western blot - Anti-XAF1 antibody [EPR26960-175] (Anti-XAF1 antibody [EPR26960-175] ab313879) at 1/1000 dilution

    Lane 1: Untreated HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: HeLa treated with /ml human IFN alpha for 24 hours whole cell lysate at 20 µg

    Lane 3: Untreated Jurkat (human t cell leukemia t lymphocyte from peripheral blood) whole cell lysate at 20 µg

    Lane 4: Jurkat treated with /ml human IFN alpha overnight whole cell lysate at 20 µg

    Lane 5: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 38, 32 kDa

  • Immunoprecipitation - Anti-XAF1 antibody [EPR26960-175] - BSA and Azide free (ab313880), expandable thumbnail

    Immunoprecipitation - Anti-XAF1 antibody [EPR26960-175] - BSA and Azide free (ab313880)

    This data was developed using Anti-XAF1 antibody [EPR26960-175] ab313879, the same antibody clone in a different buffer formulation.
    XAF1 was immunoprecipitated from 0.35 mg HeLa (human cervical adenocarcinoma epithelial cell) treated with 10 ng/ml human IFN alpha for 24 hours whole cell lysate with Anti-XAF1 antibody [EPR26960-175] ab313879 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-XAF1 antibody [EPR26960-175] ab313879 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) treated with 10 ng/ml human IFN alpha for 24 hours whole cell lysate
    Lane 2: Anti-XAF1 antibody [EPR26960-175] ab313879 IP in HeLa (human cervical adenocarcinoma epithelial cell) treated with 10 ng/ml human IFN alpha for 24 hours whole cell lysate
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-XAF1 antibody [EPR26960-175] ab313879 in HeLa treated with 10 ng/ml human IFN alpha for 24 hours whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 76 seconds

    All lanes: Immunoprecipitation - Anti-XAF1 antibody [EPR26960-175] (Anti-XAF1 antibody [EPR26960-175] ab313879) at 1/30 dilution

    All lanes: HeLa (human cervical adenocarcinoma epithelial cell) treated with 10 ng/ml human IFN alpha for 24 hours whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 76s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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