Rabbit Recombinant Monoclonal XAF1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
| sELISA | |
|---|---|
| Human | Expected |
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondria, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.
BIRC4BP, XIAPAF1, XAF1, XIAP-associated factor 1, BIRC4-binding protein
Rabbit Recombinant Monoclonal XAF1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
pH: 7.2 - 7.4
Constituents: 100% PBS
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The XAF1 protein also known as XIAP associated factor 1 plays a role in apoptosis regulation. It weighs approximately 34 kDa and exists in multiple isoforms. The protein is broadly expressed in various tissues including the liver pancreas and heart. XAF1 interacts directly with XIAP (X-linked inhibitor of apoptosis) counteracting its anti-apoptotic effects. XAF1's activity centers on promoting apoptosis by binding to XIAP and inhibiting its function.
XAF1 functions in promoting cell death through apoptosis. It forms part of the apoptosome a large protein complex important for the intrinsic pathway of apoptosis. XAF1 exerts its influence by destabilizing XIAP's interaction with caspases releasing them to activate. These interactions trigger a cascade that leads to programmed cell death vital for maintaining cellular balance and response to stress.
XAF1 plays significant role in the apoptosis and cell survival pathways. Within the intrinsic pathway XAF1 associates with Bax and caspase-3 directly impacting mitochondrial membrane permeability and caspase activation. These pathways ensure proper cell turnover and reaction to cellular damage often involving Bcl-2 family proteins and Smac/DIABLO protein for regulation as well.
XAF1's role in cancer and neurological disorders is noteworthy. In several cancer types such as colon and gastric cancers decreased XAF1 expression associates with increased cancer cell survival due to impaired apoptosis. Meanwhile abnormal regulation of XAF1 links to neurodegenerative diseases involving proteins like XIAP and caspase-9 where inhibited apoptosis can lead to accumulation of damaged cells contributing to disease progression.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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