AB307463

Anti-XAF1 antibody [EPR26960-76] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal XAF1 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

BIRC4BP, XIAPAF1, XAF1, XIAP-associated factor 1, BIRC4-binding protein

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Sandwich ELISA - Anti-XAF1 antibody [EPR26960-76] - BSA and Azide free (Detector) (AB307463)

sELISA

Supplier Data

Sandwich ELISA - Anti-XAF1 antibody [EPR26960-76] - BSA and Azide free (Detector) (AB307463)

Sandwich ELISA of ab307461 with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26960-76

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The XAF1 protein also known as XIAP associated factor 1 plays a role in apoptosis regulation. It weighs approximately 34 kDa and exists in multiple isoforms. The protein is broadly expressed in various tissues including the liver pancreas and heart. XAF1 interacts directly with XIAP (X-linked inhibitor of apoptosis) counteracting its anti-apoptotic effects. XAF1's activity centers on promoting apoptosis by binding to XIAP and inhibiting its function.

Biological function summary

XAF1 functions in promoting cell death through apoptosis. It forms part of the apoptosome a large protein complex important for the intrinsic pathway of apoptosis. XAF1 exerts its influence by destabilizing XIAP's interaction with caspases releasing them to activate. These interactions trigger a cascade that leads to programmed cell death vital for maintaining cellular balance and response to stress.

Pathways

XAF1 plays significant role in the apoptosis and cell survival pathways. Within the intrinsic pathway XAF1 associates with Bax and caspase-3 directly impacting mitochondrial membrane permeability and caspase activation. These pathways ensure proper cell turnover and reaction to cellular damage often involving Bcl-2 family proteins and Smac/DIABLO protein for regulation as well.

XAF1's role in cancer and neurological disorders is noteworthy. In several cancer types such as colon and gastric cancers decreased XAF1 expression associates with increased cancer cell survival due to impaired apoptosis. Meanwhile abnormal regulation of XAF1 links to neurodegenerative diseases involving proteins like XIAP and caspase-9 where inhibited apoptosis can lead to accumulation of damaged cells contributing to disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondria, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells.

See full target information XAF1

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-XAF1 antibody [EPR26960-76] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-XAF1 antibody [EPR26960-76] - BSA and Azide free (Detector) (AB307463)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise