AB231316

Anti-Xanthine Oxidase antibody

Name: Anti-Xanthine Oxidase antibody (ab231316) | Abcam
Brand: Abcam Limited
SKU: AB231316
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal Xanthine Oxidase antibody. Suitable for WB and reacts with Mouse, Cow samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Cow XDH aa 1-250.

View Alternative Names

Xanthine dehydrogenase/oxidase, Xdh

3 Images

Supplier Data

Western blot - Anti-Xanthine Oxidase antibody (AB231316)

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Western blot - Anti-Xanthine Oxidase antibody (ab231316) at 1 µg/mL

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Mouse liver lysate

Secondary

All lanes:

HRP-Linked Guinea pig Anti-Rabbit antibody at 1/1000 dilution

Predicted band size: 146 kDa

false

Supplier Data

Western blot - Anti-Xanthine Oxidase antibody (AB231316)

All lanes:

Western blot - Anti-Xanthine Oxidase antibody (ab231316) at 2 µg/mL

All lanes:

Cow liver lysate

Predicted band size: 146 kDa

false

Supplier Data

Western blot - Anti-Xanthine Oxidase antibody (AB231316)

All lanes:

Western blot - Anti-Xanthine Oxidase antibody (ab231316) at 2 µg/mL

All lanes:

Recombinant cow Xanthine Oxidase protein

Predicted band size: 146 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Cow

Applications

applications

Immunogen

Recombinant Fragment Protein within Cow XDH aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

P80457

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 mg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-2 mg/mL", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab231316 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography.

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 55.77% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Xanthine oxidase also known as XO is an enzyme often referred to as xanthine dehydrogenase/oxidase. It has an important role in purine metabolism converting hypoxanthine to xanthine and then xanthine to uric acid. This enzyme is an aldehyde oxidase and belongs to the family of molybdenum-containing enzymes. Xanthine oxidase has a molecular weight of approximately 290 kDa. It is expressed in various tissues predominantly in the liver and small intestine with lower levels in kidney and lung tissues.

Biological function summary

Xanthine oxidase has a significant role in the production of reactive oxygen species such as superoxide during the process of purine catabolism. In its dehydrogenase form XO can interconvert into an oxidase form engaging more readily in the electron transfer that leads to reactive oxygen species generation. Xanthine oxidase is not a part of any larger complex but functions as a dimer with two identical subunits that facilitate its metabolic roles.

Pathways

The enzyme is pivotal in the purine degradation pathway which is essential for the breakdown and eventual excretion of purine compounds as uric acid. This pathway connects it to enzymes like hypoxanthine-guanine phosphoribosyltransferase (HGPRT) which performs salvage reactions to recycle purines. Additionally xanthine oxidase's role in reactive oxygen species production links it to oxidative stress pathways impacting proteins such as superoxide dismutase which works to detoxify reactive intermediates.

Xanthine oxidase is closely implicated in conditions like gout and hyperuricemia due to its role in uric acid production. Elevated activity of XO can lead to excessive uric acid accumulation contributing to these disorders. Furthermore XO is associated with cardiovascular diseases as the generated reactive oxygen species can foster endothelial dysfunction. The enzyme’s activity is intertwined with systems involving proteins like endothelial nitric oxide synthase where oxidative stress affects nitric oxide availability and vascular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species.

See full target information Xdh

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

European journal of nutrition 62:1845-1857 PubMed36853380

2023

Long-term dietary nitrate supplementation slows the progression of established atherosclerosis in ApoE mice fed a high fat diet.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Liu,Kevin D Croft,Trevor A Mori,Tracey A Gaspari,Barbara K Kemp-Harper,Natalie C Ward

Cell metabolism 34:1548-1560.e6 PubMed36041455

2022

PIDDosome-SCAP crosstalk controls high-fructose-diet-dependent transition from simple steatosis to steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Ju Youn Kim,Lily Q Wang,Valentina C Sladky,Tae Gyu Oh,Junlai Liu,Kaitlyn Trinh,Felix Eichin,Michael Downes,Mojgan Hosseini,Etienne D Jacotot,Ronald M Evans,Andreas Villunger,Michael Karin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Xanthine Oxidase antibody

Western blot - Anti-Xanthine Oxidase antibody (AB231316)

Western blot - Anti-Xanthine Oxidase antibody (AB231316)

Western blot - Anti-Xanthine Oxidase antibody (AB231316)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise