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Rabbit Recombinant Monoclonal Xanthine Oxidase antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 29 publications.


Images

Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (AB109235), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (AB109235), expandable thumbnail
  • Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (AB109235), expandable thumbnail
  • Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (AB109235), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (AB109235), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Tested
Tested
Mouse
Expected
Tested
Rat
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/100 - 1/250

Notes

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000 - 1/10000

Notes

-

Species

Rat

Dilution info

1/1000 - 1/10000

Notes

-

Species

Human

Dilution info

1/1000 - 1/10000

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).

Alternative names

Xanthine dehydrogenase/oxidase, XDH, XDHA

Recommended products

Rabbit Recombinant Monoclonal Xanthine Oxidase antibody. Suitable for IHC-P, WB and reacts with Human, Mouse, Rat samples. Cited in 29 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR4605

Purification technique

Affinity purification Protein A

Specificity

The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Xanthine oxidase also known as XO is an enzyme often referred to as xanthine dehydrogenase/oxidase. It has an important role in purine metabolism converting hypoxanthine to xanthine and then xanthine to uric acid. This enzyme is an aldehyde oxidase and belongs to the family of molybdenum-containing enzymes. Xanthine oxidase has a molecular weight of approximately 290 kDa. It is expressed in various tissues predominantly in the liver and small intestine with lower levels in kidney and lung tissues.

Biological function summary

Xanthine oxidase has a significant role in the production of reactive oxygen species such as superoxide during the process of purine catabolism. In its dehydrogenase form XO can interconvert into an oxidase form engaging more readily in the electron transfer that leads to reactive oxygen species generation. Xanthine oxidase is not a part of any larger complex but functions as a dimer with two identical subunits that facilitate its metabolic roles.

Pathways

The enzyme is pivotal in the purine degradation pathway which is essential for the breakdown and eventual excretion of purine compounds as uric acid. This pathway connects it to enzymes like hypoxanthine-guanine phosphoribosyltransferase (HGPRT) which performs salvage reactions to recycle purines. Additionally xanthine oxidase's role in reactive oxygen species production links it to oxidative stress pathways impacting proteins such as superoxide dismutase which works to detoxify reactive intermediates.

Associated diseases and disorders

Xanthine oxidase is closely implicated in conditions like gout and hyperuricemia due to its role in uric acid production. Elevated activity of XO can lead to excessive uric acid accumulation contributing to these disorders. Furthermore XO is associated with cardiovascular diseases as the generated reactive oxygen species can foster endothelial dysfunction. The enzyme’s activity is intertwined with systems involving proteins like endothelial nitric oxide synthase where oxidative stress affects nitric oxide availability and vascular health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235), expandable thumbnail

    Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)

    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

    Xanthine Oxidase is abundantly expressed in liver and low in heart, brain and lung as what is described in PMID: 10462034

    All lanes: Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/10000 dilution

    Lane 1: Mouse liver lysates at 20 µg

    Lane 2: Rat liver lysates at 20 µg

    Lane 3: Mouse heart lysates at 20 µg

    Lane 4: Mouse brain lysates at 20 µg

    Lane 5: Mouse lung lysates at 20 µg

    Lane 6: Mouse kidney lysates at 20 µg

    Lane 7: Mouse spleen lysates at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 146 kDa

    Observed band size: 146 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue sections labeling Xanthine Oxidase with purified ab109235 at 1/100 dilution (2.3 µg/mL). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235), expandable thumbnail

    Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)

    Blocking/Diluting Buffer and concentration: 5% NFDM/TBST

    All lanes: Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/5000 dilution

    All lanes: Human milk lysates at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 146 kDa

    Observed band size: 146 kDa

  • Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235), expandable thumbnail

    Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)

    Secondary antibody - anti-rabbit HRP (Goat Anti-Rabbit IgG H&L (HRP) ab6721)

    All lanes: Western blot - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235) at 1/1000 dilution

    Lane 1: Human fetal liver lysate at 10 µg

    Lane 2: 293T lysate at 10 µg

    Lane 3: Human milk lysate at 10 µg

    Predicted band size: 146 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Xanthine Oxidase antibody [EPR4605] (ab109235)

    Immunohistochemical analysis of Xanthine Oxidase expression in paraffin-embedded Human liver tissue using ab109235 (unpurified) at 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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