AB133268

Anti-Xanthine Oxidase antibody [EPR4606]

Name: Anti-Xanthine Oxidase antibody [EPR4606] (ab133268) | Abcam
Brand: Abcam Limited
SKU: AB133268
Price: 325 USD
Availability: InStock
Rating: 5 (2 reviews)

RabMAb
Recombinant
What is this?

(2 Reviews)

(12 Publications)

Rabbit Recombinant Monoclonal Xanthine Oxidase antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 12 publications.

View Alternative Names

XDHA, XDH, Xanthine dehydrogenase/oxidase

1 Images

Unknown

Western blot - Anti-Xanthine Oxidase antibody [EPR4606] (AB133268)

All lanes:

Western blot - Anti-Xanthine Oxidase antibody [EPR4606] (ab133268) at 1/1000 dilution

Lane 1:

Human milk lysate

Lane 2:

Human liver lysate

Lane 3:

Mouse heart lysate

Lane 4:

Rat heart lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 146 kDa

false

Carrier free

Anti-Xanthine Oxidase antibody [EPR4606] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4606

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "For unpurified use at 1/1000 - 1/10000.", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Xanthine oxidase also known as XO is an enzyme often referred to as xanthine dehydrogenase/oxidase. It has an important role in purine metabolism converting hypoxanthine to xanthine and then xanthine to uric acid. This enzyme is an aldehyde oxidase and belongs to the family of molybdenum-containing enzymes. Xanthine oxidase has a molecular weight of approximately 290 kDa. It is expressed in various tissues predominantly in the liver and small intestine with lower levels in kidney and lung tissues.

Biological function summary

Xanthine oxidase has a significant role in the production of reactive oxygen species such as superoxide during the process of purine catabolism. In its dehydrogenase form XO can interconvert into an oxidase form engaging more readily in the electron transfer that leads to reactive oxygen species generation. Xanthine oxidase is not a part of any larger complex but functions as a dimer with two identical subunits that facilitate its metabolic roles.

Pathways

The enzyme is pivotal in the purine degradation pathway which is essential for the breakdown and eventual excretion of purine compounds as uric acid. This pathway connects it to enzymes like hypoxanthine-guanine phosphoribosyltransferase (HGPRT) which performs salvage reactions to recycle purines. Additionally xanthine oxidase's role in reactive oxygen species production links it to oxidative stress pathways impacting proteins such as superoxide dismutase which works to detoxify reactive intermediates.

Xanthine oxidase is closely implicated in conditions like gout and hyperuricemia due to its role in uric acid production. Elevated activity of XO can lead to excessive uric acid accumulation contributing to these disorders. Furthermore XO is associated with cardiovascular diseases as the generated reactive oxygen species can foster endothelial dysfunction. The enzyme’s activity is intertwined with systems involving proteins like endothelial nitric oxide synthase where oxidative stress affects nitric oxide availability and vascular health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).

See full target information XDH

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 82:103592 PubMed40209616

2025

Accelerating inflammatory resolution in humans to improve endothelial function and vascular health: Targeting the non-canonical pathway for NO.

Applications

Unspecified application

Species

Unspecified reactive species

Clement Lau,Christopher P Primus,Asad Shabbir,Ismita Chhetri,Mutsumi Ono,Michael Masucci,Muhammad Aadil Bin Noorany Aubdool,Julie Amarin,Alexander Jp Hamers,Zara Khan,Nitin Ajit Kumar,Shanik A Montalvo Moreira,Gani Nuredini,Miski Osman,Charlotte Whitear,Tom Godec,Vikas Kapil,Gianmichele Massimo,Rayomand S Khambata,Krishnaraj S Rathod,Amrita Ahluwalia

Redox biology 67:102864 PubMed37713777

2023

Natural mutations of human XDH promote the nitrite (NO)-reductase capacity of xanthine oxidoreductase: A novel mechanism to promote redox health?

Applications

Unspecified application

Species

Unspecified reactive species

G Massimo,R S Khambata,T Chapman,K Birchall,C Raimondi,A Shabbir,Nicki Dyson,K S Rathod,C Borghi,A Ahluwalia

Nutrients 15: PubMed36771199

2023

A Randomized Trial on Resveratrol Supplement Affecting Lipid Profile and Other Metabolic Markers in Subjects with Dyslipidemia.

Applications

Unspecified application

Species

Unspecified reactive species

Yuqing Zhou,Yupeng Zeng,Zhijun Pan,Yufeng Jin,Qing Li,Juan Pang,Xin Wang,Yu Chen,Yan Yang,Wenhua Ling

Frontiers in cell and developmental biology 10:958566 PubMed36158190

2022

Perilipin-2 promotes lipid droplet-plasma membrane interactions that facilitate apocrine lipid secretion in secretory epithelial cells of the mouse mammary gland.

Applications

Unspecified application

Species

Unspecified reactive species

Jenifer Monks,David J Orlicky,Andrew E Libby,Monica Dzieciatkowska,Mark S Ladinsky,James L McManaman

Scientific reports 11:8183 PubMed33854158

2021

Nitric oxide attenuated transforming growth factor-β induced myofibroblast differentiation of human keratocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Joo-Hee Park,Martha Kim,Bora Yim,Choul Yong Park

Nitric oxide : biology and chemistry 109-110:42-49 PubMed33713800

2021

Control of rat muscle nitrate levels after perturbation of steady state dietary nitrate intake.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Won Park,Samantha M Thomas,Alan N Schechter,Barbora Piknova

Scientific reports 10:13166 PubMed32759980

2020

Potential roles of nitrate and nitrite in nitric oxide metabolism in the eye.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Won Park,Barbora Piknova,Audrey Jenkins,David Hellinga,Leonard M Parver,Alan N Schechter

The Journal of physiology 597:5565-5576 PubMed31350908

2019

Human skeletal muscle nitrate store: influence of dietary nitrate supplementation and exercise.

Applications

Unspecified application

Species

Unspecified reactive species

Lee J Wylie,Ji Won Park,Anni Vanhatalo,Stefan Kadach,Matthew I Black,Zdravko Stoyanov,Alan N Schechter,Andrew M Jones,Barbora Piknova

Immunity 51:258-271.e5 PubMed31350176

2019

The Histone Methyltransferase Setdb2 Modulates Macrophage Phenotype and Uric Acid Production in Diabetic Wound Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew S Kimball,Frank M Davis,Aaron denDekker,Amrita D Joshi,Matthew A Schaller,Jennifer Bermick,Xianying Xing,Charles F Burant,Andrea T Obi,Dylan Nysz,Scott Robinson,Ron Allen,Nicholas W Lukacs,Peter K Henke,Johann E Gudjonsson,Bethany B Moore,Steve L Kunkel,Katherine A Gallagher

Nitric oxide : biology and chemistry 91:1-14 PubMed31299340

2019

Inhaled nebulized nitrite and nitrate therapy in a canine model of hypoxia-induced pulmonary hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Cortés-Puch,Junfeng Sun,Alan N Schechter,Steven B Solomon,Ji Won Park,Jing Feng,Cameron Gilliard,Charles Natanson,Barbora Piknova

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Xanthine Oxidase antibody [EPR4606]

Western blot - Anti-Xanthine Oxidase antibody [EPR4606] (AB133268)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (12)

Product promise