Rabbit Recombinant Monoclonal Xanthine Oxidase antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
IP | Flow Cyt | WB | ICC/IF | |
---|---|---|---|---|
Human | Not recommended | Not recommended | Tested | Not recommended |
Mouse | Not recommended | Not recommended | Tested | Not recommended |
Rat | Not recommended | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
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Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat, Human | Dilution info - | Notes - |
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Key enzyme in purine degradation. Catalyzes the oxidation of hypoxanthine to xanthine. Catalyzes the oxidation of xanthine to uric acid. Contributes to the generation of reactive oxygen species. Has also low oxidase activity towards aldehydes (in vitro).
XDHA, XDH, XDHA, Xanthine dehydrogenase/oxidase
Rabbit Recombinant Monoclonal Xanthine Oxidase antibody. Carrier free. Suitable for WB and reacts with Mouse, Rat, Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR4606
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
ab248434 is the carrier-free version of Anti-Xanthine Oxidase antibody [EPR4606] ab133268.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
Xanthine oxidase also known as XO is an enzyme often referred to as xanthine dehydrogenase/oxidase. It has an important role in purine metabolism converting hypoxanthine to xanthine and then xanthine to uric acid. This enzyme is an aldehyde oxidase and belongs to the family of molybdenum-containing enzymes. Xanthine oxidase has a molecular weight of approximately 290 kDa. It is expressed in various tissues predominantly in the liver and small intestine with lower levels in kidney and lung tissues.
Xanthine oxidase has a significant role in the production of reactive oxygen species such as superoxide during the process of purine catabolism. In its dehydrogenase form XO can interconvert into an oxidase form engaging more readily in the electron transfer that leads to reactive oxygen species generation. Xanthine oxidase is not a part of any larger complex but functions as a dimer with two identical subunits that facilitate its metabolic roles.
The enzyme is pivotal in the purine degradation pathway which is essential for the breakdown and eventual excretion of purine compounds as uric acid. This pathway connects it to enzymes like hypoxanthine-guanine phosphoribosyltransferase (HGPRT) which performs salvage reactions to recycle purines. Additionally xanthine oxidase's role in reactive oxygen species production links it to oxidative stress pathways impacting proteins such as superoxide dismutase which works to detoxify reactive intermediates.
Xanthine oxidase is closely implicated in conditions like gout and hyperuricemia due to its role in uric acid production. Elevated activity of XO can lead to excessive uric acid accumulation contributing to these disorders. Furthermore XO is associated with cardiovascular diseases as the generated reactive oxygen species can foster endothelial dysfunction. The enzyme’s activity is intertwined with systems involving proteins like endothelial nitric oxide synthase where oxidative stress affects nitric oxide availability and vascular health.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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All lanes: Western blot - Anti-Xanthine Oxidase antibody [EPR4606] (Anti-Xanthine Oxidase antibody [EPR4606] ab133268) at 1/1000 dilution
Lane 1: Human milk lysate
Lane 2: Human liver lysate
Lane 3: Mouse heart lysate
Lane 4: Rat heart lysate
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 146 kDa
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