Anti-XBP1 antibody

• IHC (PFA fixed)

Supplier Data

Immunohistochemistry (PFA fixed) - Anti-XBP1 antibody (AB37152)

Immunohistochemistry of human liver tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).

• IHC-P

PubMed

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (AB37152)

ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.

Image from Ren Yunqing et.al., PLoS Genet. 2009 June; 5(6): e1000523 (Fig 6C).

• IHC-P

AbReview22892****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (AB37152)

ab37152 staining XBP1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate-EDTA antigen retrieval buffer and microwave. Heating was performed in the microwave 3 minutes on high until boiling, 6 minutes on 20% power. Samples were then blocked for 15 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 12 hours at 4°C. An undiluted HRP-conjugated rat polyclonal was used as the secondary antibody.

Image courtesy of an anonymous Abreview.

• IHC (PFA fixed)

Supplier Data

Immunohistochemistry (PFA fixed) - Anti-XBP1 antibody (AB37152)

Immunohistochemistry of human pancreas tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody (AB37152)

Immunofluorescent analysis of methanol-fixed HeLa cells labeling XBP1 with ab37152 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (AB37152)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using ab37152 at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary antibody. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (AB37152)

Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using ab37152 at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary antibody. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (AB37152)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using ab37152 at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary antibody. Counter stained with Hematoxylin.

• WB

Supplier Data

Western blot - Anti-XBP1 antibody (AB37152)

Lane 1:

Western blot - Anti-XBP1 antibody (ab37152) at 0.5 µg/mL

Lane 2:

Western blot - Anti-XBP1 antibody (ab37152) at 1 µg/mL

Lane 3:

Western blot - Anti-XBP1 antibody (ab37152) at 2 µg/mL

All lanes:

Human XBP-1 recombinant protein at 100 ng

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

false

• WB

Supplier Data

Western blot - Anti-XBP1 antibody (AB37152)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-XBP1 antibody (ab37152) at 4 µg/mL

Lane 1:

WT 293T Cells at 10 µg/mL

Lane 2:

XBP-1 knockout 293T Cells at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 29 kDa

false

• WB

Supplier Data

Western blot - Anti-XBP1 antibody (AB37152)

1h incubation at RT in 5% NFDM/TBST.

Lane 1:

Western blot - Anti-XBP1 antibody (ab37152) at 0.25 µg/mL

Lane 2:

Western blot - Anti-XBP1 antibody (ab37152) at 0.5 µg/mL

Lane 3:

Western blot - Anti-XBP1 antibody (ab37152) at 1 µg/mL

All lanes:

A549 cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 29 kDa

false

• WB

Supplier Data

Western blot - Anti-XBP1 antibody (AB37152)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-XBP1 antibody (ab37152) at 4 µg/mL

Lane 1:

Wild Type HeLa cells at 10 µg

Lane 2:

XBP-1 knockout HeLa cells at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 29 kDa

false

• WB

Supplier Data

Western blot - Anti-XBP1 antibody (AB37152)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-XBP1 antibody (ab37152) at 1 µg/mL

Lane 1:

Rat Liver Tissue Lysate at 15 µg

Lane 2:

Rat Liver Tissue Lysate at 15 µg with Blocking peptide

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 29 kDa

false

• WB

Supplier Data

Western blot - Anti-XBP1 antibody (AB37152)

1h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-XBP1 antibody (ab37152) at 4 µg/mL

All lanes:

Mouse Pancreas Tissue lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 29 kDa

false

• WB

Supplier Data

Western blot - Anti-XBP1 antibody (AB37152)

Lane 1:

Western blot - Anti-XBP1 antibody (ab37152) at 1 µg/mL

Lane 2:

Western blot - Anti-XBP1 antibody (ab37152) at 2 µg/mL

All lanes:

HepG2 cell lysate at 15 µg

Secondary

All lanes:

Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 29 kDa

false