Anti-XBP1 antibody is a rabbit polyclonal antibody that is used to detect XBP1 in ICC, ICC/IF, IHC (PFA fixed), IHC-Fr, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Recognizes both isoforms (non-spliced and spliced) of XBP-1
- Cited in over 160 publications
- Trusted since 2006
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Publications
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- Nature cell biology 25:1804-18202023COPI vesicle formation and N-myristoylation are targetable vulnerabilities of senescent cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDomhnall McHugh,Bin Sun,Carmen Gutierrez-Muñoz,Fernanda Hernández-González,Massimiliano Mellone,Romain Guiho,Imanol Duran,Joaquim Pombo,Federico Pietrocola,Jodie Birch,Wouter W Kallemeijn,Sanjay Khadayate,Gopuraja Dharmalingam,Santiago Vernia,Edward W Tate,Juan Pedro Martínez-Barbera,Dominic J Withers,Gareth J Thomas,Manuel Serrano,Jesús GilPubMed 38012402
- Heliyon 9:e198062023GRP78 inhibitor HA15 increases the effect of Bortezomib on eradicating multiple myeloma cells through triggering endoplasmic reticulum stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYirong Chen,Yuchen Tao,Kexin Hu,Jiahui LuPubMed 37809599
- Open medicine (Warsaw, Poland) 18:202307772023miR-199a-5p inhibits aortic valve calcification by targeting ATF6 and GRP78 in valve interstitial cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHeng Chu,XingLi Fan,Zhe Zhang,Lin HanPubMed 37693833
- PloS one 18:e02896272023Association of cyclooxygenase-2 expression with endoplasmic reticulum stress and autophagy in triple-negative breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaechan Lee,SungWook Jung,Gyungyub Gong,Bora Lim,Hee Jin LeePubMed 37540709
- Aging and disease 14:1458-14712023PARP16-Mediated Stabilization of Amyloid Precursor Protein mRNA Exacerbates Alzheimer's Disease Pathogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinghuan Wang,Qianwen Cheng,Yuyu Zhang,Chen Hong,Jiayao Liu,Xinhua Liu,Jun ChangPubMed 37163422
- Cellular and molecular life sciences : CMLS 80:1612023Poly (ADP-ribose) polymerases 16 triggers pathological cardiac hypertrophy via activating IRE1α-sXBP1-GATA4 pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaibi Su,Jie Xu,Zhenghua Su,Chenxi Xiao,Jinghuan Wang,Wen Zhong,Chen Meng,Di Yang,Yizhun ZhuPubMed 37219631
- Human genome variation 10:132023A novel variant in NBAS identified from an infant with fever-triggered recurrent acute liver failure disrupts the function of the gene.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuhua Ji,Mingming Yang,JunJun Jia,Qi Wu,Ruochen Cong,Hengxiang Cui,Baofeng Zhu,Xin ChuPubMed 37055399
- International journal of molecular sciences 24:2023MSX1 Regulates Goat Endometrial Function by Altering the Plasma Membrane Transformation of Endometrial Epithelium Cells during Early Pregnancy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBeibei Zhang,Zongjie Wang,Kangkang Gao,Rao Fu,Huatao Chen,Pengfei Lin,Aihua Wang,Yaping JinPubMed 36835532
- CNS neuroscience & therapeutics 29:1049-10662023Human-induced pluripotent stem cell-derived cerebral organoid of leukoencephalopathy with vanishing white matter.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiong Deng,Jie Zhang,Kai Gao,Ling Zhou,Yuwu Jiang,Jingmin Wang,Ye WuPubMed 36650674
- Clinical and translational medicine 13:e11662023XBP1-elicited environment by chemotherapy potentiates repopulation of tongue cancer cells by enhancing miR-22/lncRNA/KAT6B-dependent NF-κB signalling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoting Jia,Ge Wang,Lihong Wu,Hao Pan,Li Ling,Jianlei Zhang,Qingquan Wen,Jie Cui,Zhimin He,Bin Qi,Shuxu Zhang,Liyun Luo,Guopei ZhengPubMed 36639835
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human XBP1. The exact immunogen used to generate this antibody is proprietary information. Database link P17861
Consider this alternative
Reactivity data
Select an application| WB | IHC (PFA fixed) | IHC-P | ICC | ICC/IF | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Expected | Tested | Expected |
| Mouse | Tested | Tested | Tested | Predicted | Expected | Tested |
| Rat | Tested | Expected | Tested | Predicted | Expected | Expected |
| Cow | Predicted | Predicted | Predicted | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 0.5-2 µg/mL | Notes Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 29 kDa (isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910). |
Species Rat | Dilution info 0.5-2 µg/mL | Notes Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 29 kDa (isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910). |
Species Human | Dilution info 0.5-2 µg/mL | Notes Use at a concentration of 1 - 2 μg/ml. Predicted molecular weight: 29 kDa (isoform 1) and 40 kD (isoform 2). Can be blocked with XBP1 peptide (ab39910). |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC (PFA fixed)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 20 µg/mL | Notes - |
Species Human | Dilution info 20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 2-5 µg/mL | Notes - |
Species Mouse | Dilution info 2-5 µg/mL | Notes - |
Species Human | Dilution info 2-5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Cow, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 20 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
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Target data
Function
Functions as a transcription factor during endoplasmic reticulum (ER) stress by regulating the unfolded protein response (UPR). Required for cardiac myogenesis and hepatogenesis during embryonic development, and the development of secretory tissues such as exocrine pancreas and salivary gland (By similarity). Involved in terminal differentiation of B lymphocytes to plasma cells and production of immunoglobulins (PubMed:11460154). Modulates the cellular response to ER stress in a PIK3R-dependent manner (PubMed:20348923). Binds to the cis-acting X box present in the promoter regions of major histocompatibility complex class II genes (PubMed:8349596). Involved in VEGF-induced endothelial cell (EC) proliferation and retinal blood vessel formation during embryonic development but also for angiogenesis in adult tissues under ischemic conditions. Functions also as a major regulator of the UPR in obesity-induced insulin resistance and type 2 diabetes for the management of obesity and diabetes prevention (By similarity). Isoform 1. Plays a role in the unconventional cytoplasmic splicing processing of its own mRNA triggered by the endoplasmic reticulum (ER) transmembrane endoribonuclease ERN1: upon ER stress, the emerging XBP1 polypeptide chain, as part of a mRNA-ribosome-nascent chain (R-RNC) complex, cotranslationally recruits its own unprocessed mRNA through transient docking to the ER membrane and translational pausing, therefore facilitating efficient IRE1-mediated XBP1 mRNA isoform 2 production (PubMed:19394296, PubMed:21233347). In endothelial cells (EC), associated with KDR, promotes IRE1-mediated XBP1 mRNA isoform 2 productions in a vascular endothelial growth factor (VEGF)-dependent manner, leading to EC proliferation and angiogenesis (PubMed:23529610). Functions as a negative feed-back regulator of the potent transcription factor XBP1 isoform 2 protein levels through proteasome-mediated degradation, thus preventing the constitutive activation of the ER stress response signaling pathway (PubMed:16461360, PubMed:25239945). Inhibits the transactivation activity of XBP1 isoform 2 in myeloma cells (By similarity). Acts as a weak transcriptional factor (PubMed:8657566). Together with HDAC3, contributes to the activation of NFE2L2-mediated HMOX1 transcription factor gene expression in a PI(3)K/mTORC2/Akt-dependent signaling pathway leading to EC survival under disturbed flow/oxidative stress (PubMed:25190803). Binds to the ER stress response element (ERSE) upon ER stress (PubMed:11779464). Binds to the consensus 5'-GATGACGTG[TG]N(3)[AT]T-3' sequence related to cAMP responsive element (CRE)-like sequences (PubMed:8657566). Binds the Tax-responsive element (TRE) present in the long terminal repeat (LTR) of T-cell leukemia virus type 1 (HTLV-I) and to the TPA response elements (TRE) (PubMed:1903538, PubMed:2196176, PubMed:2321018, PubMed:8657566). Associates preferentially to the HDAC3 gene promoter region in a static flow-dependent manner (PubMed:25190803). Binds to the CDH5/VE-cadherin gene promoter region (PubMed:19416856). Isoform 2. Functions as a stress-inducible potent transcriptional activator during endoplasmic reticulum (ER) stress by inducing unfolded protein response (UPR) target genes via binding to the UPR element (UPRE). Up-regulates target genes encoding ER chaperones and ER-associated degradation (ERAD) components to enhance the capacity of productive folding and degradation mechanism, respectively, in order to maintain the homeostasis of the ER under ER stress (PubMed:11779464, PubMed:25239945). Plays a role in the production of immunoglobulins and interleukin-6 in the presence of stimuli required for plasma cell differentiation (By similarity). Induces phospholipid biosynthesis and ER expansion (PubMed:15466483). Contributes to the VEGF-induced endothelial cell (EC) growth and proliferation in a Akt/GSK-dependent and/or -independent signaling pathway, respectively, leading to beta-catenin nuclear translocation and E2F2 gene expression (PubMed:23529610). Promotes umbilical vein EC apoptosis and atherosclerotisis development in a caspase-dependent signaling pathway, and contributes to VEGF-induced EC proliferation and angiogenesis in adult tissues under ischemic conditions (PubMed:19416856, PubMed:23529610). Involved in the regulation of endostatin-induced autophagy in EC through BECN1 transcriptional activation (PubMed:23184933). Plays a role as an oncogene by promoting tumor progression: stimulates zinc finger protein SNAI1 transcription to induce epithelial-to-mesenchymal (EMT) transition, cell migration and invasion of breast cancer cells (PubMed:25280941). Involved in adipocyte differentiation by regulating lipogenic gene expression during lactation. Plays a role in the survival of both dopaminergic neurons of the substantia nigra pars compacta (SNpc), by maintaining protein homeostasis and of myeloma cells. Increases insulin sensitivity in the liver as a response to a high carbohydrate diet, resulting in improved glucose tolerance. Improves also glucose homeostasis in an ER stress- and/or insulin-independent manner through both binding and proteasome-induced degradation of the transcription factor FOXO1, hence resulting in suppression of gluconeogenic genes expression and in a reduction of blood glucose levels. Controls the induction of de novo fatty acid synthesis in hepatocytes by regulating the expression of a subset of lipogenic genes in an ER stress- and UPR-independent manner (By similarity). Associates preferentially to the HDAC3 gene promoter region in a disturbed flow-dependent manner (PubMed:25190803). Binds to the BECN1 gene promoter region (PubMed:23184933). Binds to the CDH5/VE-cadherin gene promoter region (PubMed:19416856). Binds to the ER stress response element (ERSE) upon ER stress (PubMed:11779464). Binds to the 5'-CCACG-3' motif in the PPARG promoter (By similarity).
Alternative names
TREB5, XBP2, XBP1, X-box-binding protein 1, XBP-1, Tax-responsive element-binding protein 5, TREB-5
Recommended products
Anti-XBP1 antibody is a rabbit polyclonal antibody that is used to detect XBP1 in ICC, ICC/IF, IHC (PFA fixed), IHC-Fr, IHC-P, Western blot. Suitable for Human, Mouse, Rat samples.
- Recognizes both isoforms (non-spliced and spliced) of XBP-1
- Cited in over 160 publications
- Trusted since 2006
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human XBP1. The exact immunogen used to generate this antibody is proprietary information. Database link P17861
- Purification technique
- Affinity purification Immunogen
- Specificity
XBP-1 antibody will recognize both isoforms (non-spliced and spliced) of XBP-1.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-XBP1 antibody (ab37152) is a rabbit polyclonal antibody and is validated for use in ICC, ICC/IF, IHC (PFA fixed), IHC-Fr, IHC-P, WB in human, mouse, rat samples.
Anti-XBP1 antibody (ab37152) specifically detects XBP1 (UniProt ID: P17861; Molecular weight: 29kDa) and is sold in 100 µg selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-XBP1 antibody (ab37152) has high sensitivity and specificity.
Anti-XBP1 antibody (ab37152) has been cited over 165 times in peer reviewed journals and is trusted by the scientific community.
Anti-XBP1 antibody (ab37152) has 28 independent reviews from customers.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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15 product images
Immunohistochemistry (PFA fixed) - Anti-XBP1 antibody (ab37152)
Immunohistochemistry of human liver tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).
Image from Ren Yunqing et.al., PLoS Genet. 2009 June; 5(6): e1000523 (Fig 6C).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
ab37152 at 1/200 dilution staining XBP1 in human non-lesional skin tissue sections by Immunohistochemistry (Formalin/ PFA fixed paraformaldehyde tissue sections). A biotin labelled secondary was used at 1/100 dilution. Diaminobenzidine (DAB) was used for staining development in a ABC staining Kit and the sections were counterstained with haematoxylin.
Western blot - Anti-XBP1 antibody (ab37152)
Lane 1: Western blot - Anti-XBP1 antibody (ab37152) at 1 µg/mL
Lane 2: Western blot - Anti-XBP1 antibody (ab37152) at 2 µg/mL
All lanes: HepG2 cell lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa
Immunohistochemistry (PFA fixed) - Anti-XBP1 antibody (ab37152)
Immunohistochemistry of human pancreas tissue labeling XBP1 with Anti-XBP1 antibody (ab37152) at 20μg/ml. followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green). DAPI staining (Blue).
Image courtesy of an anonymous customer review.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
ab37152 staining XBP1 in human liver tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using Citrate-EDTA antigen retrieval buffer and microwave. Heating was performed in the microwave 3 minutes on high until boiling, 6 minutes on 20% power. Samples were then blocked for 15 minutes at 27°C followed by incubation with the primary antibody at a 1/100 dilution for 12 hours at 4°C. An undiluted HRP-conjugated rat polyclonal was used as the secondary antibody.
Western blot - Anti-XBP1 antibody (ab37152)
1h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-XBP1 antibody (ab37152) at 4 µg/mL
Lane 1: Wild Type HeLa cells at 10 µg
Lane 2: XBP-1 knockout HeLa cells at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue using ab37152 at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary antibody. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue using ab37152 at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary antibody. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XBP1 antibody (ab37152)
Immunohistochemical analysis of paraffin-embedded Rat spleen tissue using ab37152 at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 dilution was used as secondary antibody. Counter stained with Hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-XBP1 antibody (ab37152)
Immunofluorescent analysis of methanol-fixed HeLa cells labeling XBP1 with ab37152 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/1000 dilution (red) and DAPI staining (blue). Alpha tubulin was stained with anti-alpha tubulin antibody following by goat anti-mouse IgG secondary antibody (green). Images were captured with confocal microscopy.
Western blot - Anti-XBP1 antibody (ab37152)
1h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-XBP1 antibody (ab37152) at 4 µg/mL
Lane 1: WT 293T Cells at 10 µg/mL
Lane 2: XBP-1 knockout 293T Cells at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa
Western blot - Anti-XBP1 antibody (ab37152)
1h incubation at RT in 5% NFDM/TBST.
Lane 1: Western blot - Anti-XBP1 antibody (ab37152) at 0.25 µg/mL
Lane 2: Western blot - Anti-XBP1 antibody (ab37152) at 0.5 µg/mL
Lane 3: Western blot - Anti-XBP1 antibody (ab37152) at 1 µg/mL
All lanes: A549 cell lysate at 15 µg
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa
Western blot - Anti-XBP1 antibody (ab37152)
Lane 1: Western blot - Anti-XBP1 antibody (ab37152) at 0.5 µg/mL
Lane 2: Western blot - Anti-XBP1 antibody (ab37152) at 1 µg/mL
Lane 3: Western blot - Anti-XBP1 antibody (ab37152) at 2 µg/mL
All lanes: Human XBP-1 recombinant protein at 100 ng
SecondaryAll lanes: Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Western blot - Anti-XBP1 antibody (ab37152)
1h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-XBP1 antibody (ab37152) at 1 µg/mL
Lane 1: Rat Liver Tissue Lysate at 15 µg
Lane 2: Rat Liver Tissue Lysate at 15 µg with Blocking peptide
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa
Western blot - Anti-XBP1 antibody (ab37152)
1h incubation at RT in 5% NFDM/TBST.
All lanes: Western blot - Anti-XBP1 antibody (ab37152) at 4 µg/mL
All lanes: Mouse Pancreas Tissue lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 29 kDa
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