Rabbit Recombinant Monoclonal XCL1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Images
![Sandwich ELISA - Anti-XCL1 antibody [EPR22634-115] - BSA and Azide free (Capture) (AB270320), expandable thumbnail](https://content.abcam.com/products/images/xcl1-antibody-epr22634-115-bsa-and-azide-free-capture-ab270320--sandwich-elisa-img46959.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-XCL1 antibody [EPR22634-115] - BSA and Azide free (Capture) (AB270320)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Mouse | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Chemotactic activity for lymphocytes but not for monocytes or neutrophils. In thymus, mediates medullary accumulation of thymic dendritic cells and contributes to regulatoy T cell development, playing a role in self-tolerance establishment.
Alternative names
Lptn, Ltn, Scyc1, Xcl1, Lymphotactin, C motif chemokine 1, Cytokine SCM-1, Lymphotaxin, Small-inducible cytokine C1
Recommended products
Rabbit Recombinant Monoclonal XCL1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22634-115
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab270320 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse XCL1. The recommended pair for sandwich ELISA is:
Capture: ab270320, Mouse XCL1 Capture Antibody (unconjugated)
Detector: Anti-XCL1 antibody [EPR22634-114] - BSA and Azide free (Detector) ab270321, Mouse XCL1 Detector Antibody (unconjugated)
The reference range value is 18.75 - 1200 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
XCL1 also known as lymphotactin is a chemokine with a molecular mass of approximately 10 kDa. This protein plays a role in the immune system directing the movement of cells such as T lymphocytes to sites of inflammation or infection. XCL1 is mainly expressed in activated T cells natural killer cells and some other immune cells. Its expression gets upregulated in response to certain stimuli which helps to recruit additional immune effector cells to areas where they are needed.
- Biological function summary
XCL1 participates in chemotaxis guiding the migration of specific cells to enhance the immune response. Although XCL1 does not form part of a larger protein complex it interacts with its specific receptor XCR1 on target cells. This interaction triggers intracellular signaling pathways contributing to the controlled movement and positioning of immune cells in response to infection or inflammatory signals.
- Pathways
XCL1 is significantly involved in the chemokine signaling pathway and plays a part in immune response regulation. This pathway shared with other chemokines like CCL2 and CCL5 orchestrates the precise movement and activity of immune cells. XCL1’s interaction with XCR1 is central to initiating downstream signaling processes which influence immune responses and inflammation.
- Associated diseases and disorders
XCL1 associates with autoimmune diseases and certain viral infections. Its dysregulation appears in conditions such as rheumatoid arthritis where the abnormal control of immune cells contributes to inflammation and tissue damage. In viral infections like HIV XCL1 helps modulate immune cell responses against the virus. XCR1 the receptor of XCL1 also plays a role in these diseases being a critical component in mediating the effects of lymphotactin in varied pathological contexts.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-XCL1 antibody [EPR22634-115] - BSA and Azide free (Capture) (ab270320), expandable thumbnail](https://content.abcam.com/products/images/xcl1-antibody-epr22634-115-bsa-and-azide-free-capture-ab270320--sandwich-elisa-img46959.jpg "Sandwich ELISA - Anti-XCL1 antibody [EPR22634-115] - BSA and Azide free (Capture) (ab270320)")
Sandwich ELISA - Anti-XCL1 antibody [EPR22634-115] - BSA and Azide free (Capture) (ab270320)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Mouse XCL1 ELISA Kit (Lymphotactin) ab269540).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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