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AB300667

Anti-xCT antibody [3A12]

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(3 Publications)

Rat Recombinant Monoclonal XCT antibody. Suitable for WB, ICC/IF, IP and reacts with Human samples. Cited in 3 publications.

View Alternative Names

Cystine/glutamate transporter, Amino acid transport system xc-, Calcium channel blocker resistance protein CCBR1, Solute carrier family 7 member 11, xCT, SLC7A11

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-xCT antibody [3A12] (AB300667)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-xCT antibody [3A12] (AB300667)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized A549 (human lung carcinoma epithelial cell) cells labelling xCT with ab300667 at 1/50 (21.18 ug/ml) dilution, followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing membraneous and cytoplasmic staining in A549 cell line.Low expression : colo 205 (PMID : 31000598). is observed. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 10 ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at 1/1000 2 ug/ml dilution.

Immunoprecipitation - Anti-xCT antibody [3A12] (AB300667)
  • IP

Supplier Data

Immunoprecipitation - Anti-xCT antibody [3A12] (AB300667)

xCT was immunoprecipitated from 0.35 mg HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug with ab300667 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300667 at 1/1000 dilution. Goat Anti-Rat IgG (H+L), HRP) (ab205720) was used at 1/10000 dilution. Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate 10 ug Lane 2 : ab300667 IP in HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate Lane 3 : Rat IgG2a, kappa monoclonal- Isotype Control (ab18450) instead of ab300667 in HepG2 whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 3 seconds

Western blot - Anti-xCT antibody [3A12] (AB300667)
  • WB

Supplier Data

Western blot - Anti-xCT antibody [3A12] (AB300667)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Low expression : SW480 and colo 205 (PMID : 31000598). Samples are non-boiled as boiling may cause protein aggregation. The expression profile observed is consistent with what has been described in the literature ( PMID : 32188872). Exposure time : 26 seconds

All lanes:

Western blot - Anti-xCT antibody [3A12] (ab300667) at 1/1000 dilution

Lane 1:

A549 (human lung carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

SW480 (human colorectal adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 5:

COLO 205 (human colon; derived from metastatic site: ascites epithelial), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rat IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rat-igg-h-l-hrp-ab205720'>ab205720</a>) at 1/10000 dilution

Observed band size: 55 kDa,35 kDa

false

Exposure time: 26s

  • Carrier free

    Anti-xCT antibody [3A12] (BSA and Azide free)

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

3A12

Isotype

IgG2a

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Heterodimer with SLC3A2, that functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane (PubMed : 11133847, PubMed : 11417227, PubMed : 14722095, PubMed : 15151999, PubMed : 34880232, PubMed : 35245456, PubMed : 35352032). Provides L-cystine for the maintenance of the redox balance between extracellular L-cystine and L-cysteine and for the maintenance of the intracellular levels of glutathione that is essential for cells protection from oxidative stress (By similarity). The transport is sodium-independent, electroneutral with a stoichiometry of 1 : 1, and is drove by the high intracellular concentration of L-glutamate and the intracellular reduction of L-cystine (PubMed : 11133847, PubMed : 11417227). In addition, mediates the import of L-kynurenine leading to anti-ferroptotic signaling propagation required to maintain L-cystine and glutathione homeostasis (PubMed : 35245456). Moreover, mediates N-acetyl-L-cysteine uptake into the placenta leading to subsequently down-regulation of pathways associated with oxidative stress, inflammation and apoptosis (PubMed : 34120018). In vitro can also transport L-aspartate (PubMed : 11417227). May participate in astrocyte and meningeal cell proliferation during development and can provide neuroprotection by promoting glutathione synthesis and delivery from non-neuronal cells such as astrocytes and meningeal cells to immature neurons (By similarity). Controls the production of pheomelanin pigment directly (By similarity).
See full target information Cystine/glutamate transporter
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Redox biology 75:103270 PubMed39047638

2024

LACTB suppresses liver cancer progression through regulation of ferroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kaixuan Zeng,Na Huang,Nanbin Liu,Xi Deng,Yanhua Mu,Xurui Zhang,Jian Zhang,Chongyu Zhang,Yong Li,Zongfang Li

BMC pulmonary medicine 23:360 PubMed37749553

2023

Metformin induces ferroptosis through the Nrf2/HO-1 signaling in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Chengmin Deng,Lin Xiong,Yang Chen,Kaifeng Wu,Jie Wu

Drug design, development and therapy 17:2495-2511 PubMed37637264

2023

Galangin Attenuates Myocardial Ischemic Reperfusion-Induced Ferroptosis by Targeting Nrf2/Gpx4 Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tao Yang,Haiqiong Liu,Chaobo Yang,Huaqiang Mo,Xianbao Wang,Xudong Song,Luping Jiang,Ping Deng,Ran Chen,Pengcui Wu,Aihua Chen,Jing Yan
View all publications
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Product promise

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